Growth and survival of uninjured and sublethally heat-injured &lt;i&gt;Escherichia coli&lt;/i&gt; O157:H7 on beef extract medium as influenced by package atmosphere and storage temperature

Aims: We discuss several factors that are critical for heat inactivation experiments and which should be taken into account for future research. Methods and Results: On the basis of examples from the literature we discuss critical factors in¯uencing the calculated heat inactivation of Mycobacterium avium subsp. paratuberculosis (MAP). Furthermore, using a modelling approach, we show that tailing of the inactivation curve of MAP is caused by the presence of cell clumps and not by a more heat-resistant cell fraction. Conclusions: The experimental conditions of the MAP heat inactivation studies of different research groups vary signi®cantly and lead to considerable differences in results and conclusions. Therefore, a more consensual approach should be employed in future studies. In addition, our model on clumping of MAP can be used to predict the decimal reduction of MAP during heat treatment and to study the effect of clumping on other lethal effects. Signi®cance and Impact of the Study: We discuss several factors that should be carefully considered in heat resistance experiments. This is essential for a thorough interpretation of results from experiments and should be given proper attention in future experiments and publications on this topic.

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Heat inactivation data for Mycobacterium avium subsp. paratuberculosis: implications for interpretation

Klijn

Herrewegh

Jong

2001

J Appl Microbiol

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Inactivation of Foodborne Pathogens Using A One Atmosphere Uniform Glow Discharge Plasma

Kayes

Critzer

Kelly-Wintenberg

et al. 2007

Foodborne Pathogens and Disease

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This study was conducted to determine the efficacy of a one atmosphere uniform glow discharge plasma (OAUGDP) for inactivation of foodborne pathogens and to evaluate the influence of growth temperature, pH, and culture age on their inactivation. Escherichia coli O157:H7, Listeria monocytogenes, Staphylococcus aureus, Bacillus cereus, Salmonella Enteritidis, Vibrio parahaemolyticus, Yersinia enterocolitica, and Shigella flexneri were evaluated. Three-strain mixtures of each bacterium were inoculated (6-7 log CFU/cm(2)) onto microscope slides containing nonselective agar media adjusted to pH 5 or 7. Samples were exposed to plasma for 0-240 sec immediately, or after incubation for 24 h at 10 degrees C or 35 degrees C. After exposure, the agar was removed from the slides and pummeled in 0.1% peptone water with a stomacher, serially diluted, surface plated onto nonselective media, and incubated at 35 degrees C. Exposure time, pH, incubation temperature, and culture age affected survival of all pathogens exposed to plasma (P < 0.05). The greatest reduction of pathogens generally occurred during the initial exposure time of 30 or 90 sec. Pathogens incubated for 24 h before exposure were more resistant than those exposed immediately after inoculation. Incubation at 35 degrees C before exposure resulted in greater resistance to plasma inactivation than incubation at 10 degrees C. No appreciable differences between gram-positive and gram-negative pathogens were observed, although the spore-forming B. cereus was more resistant to plasma than non-spore-formers. These findings support the potential for plasma treatment of foods or surfaces for pathogen reduction. Increased sensitivity of pathogens to plasma at reduced pH and temperature is encouraging, since these conditions are applicable to many foods during processing, handling, and storage.

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Growth and survival of uninjured and sublethally heat-injured <i>Escherichia coli</i> O157:H7 on beef extract medium as influenced by package atmosphere and storage temperature

Cited by 2 publications

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Heat inactivation data for Mycobacterium avium subsp. paratuberculosis: implications for interpretation

Heat inactivation data for Mycobacterium avium subsp. paratuberculosis: implications for interpretation

Inactivation of Foodborne Pathogens Using A One Atmosphere Uniform Glow Discharge Plasma

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