Growth and Replication of Infectious Bursal Disease Virus in the DF-1 Cell Line and Chicken Embryo Fibroblasts

Abstract

Infectious bursal disease virus (IBDV) causes a highly contagious disease in young chicks and leads to significant economic losses in the poultry industry. To determine a suitable cell line for IBDV infection, replication, and growth kinetics of the virus, DF-1 cells and chicken embryo fibroblasts (CEF) were used. The population doubling per day (Pd/D) was found to be higher in DF-1 as compared to CEF cells. A suitable time of infection (TOI) was established for increased production of virus and greater infectivity titers. The DF-1 and CEF cells were found to be susceptible to infection by producing marked cytopathic effects (CPEs), and the growth curves of IBDV in DF-1 and CEF cells were evaluated by infectivity assay using tissue culture infectious dose (TCID50). The cytopathic effects of the virus in DF-1 and CEF cells were found to be similar, but higher viral titers were detected in the DF-1 cells as compared to CEF. Thus the DF-1 cell line had a higher growth potential and infectivity, which will be of advantage in vaccine production.

Induction of hairy roots by Agrobacterium rhizogenes -mediated transformation of spine gourd ( Momordica dioica Roxb. ex. willd) for the assessment of phenolic compounds and biological activities

“…IBD virus (IBDV) was adapted in chicken embryo fibroblast (CEF) cultures. The DF1 cells and maintenance, IBDV propagation and harvesting procedure were described previously by Rekha et al (2014).…”

Propagation and Molecular Characterization of Bioreactor Adapted Very Virulent Infectious Bursal Disease Virus Isolates of Malaysia

“…Several cell lines are in use in the development of cell culture-based vaccines among which there are Vero [ 12 , 13 ], CHO [ 12 , 14 ], RK-13 (Rinaldi et al ., 1972), and BGM-70 cells [ 12 , 14 , 15 ]. Several cell lines were utilized for producing IBD vaccines in a cell culture-based system [ 16 , 17 ]. The cell line BGM-70 derived from baby grivet monkey was reported to support the growth and rapidly attenuate classical, variant, and very virulent IBDV strains and with high viral titer [ 6 , 15 , 18 ].…”

Histopathological and immunohistochemical diagnosis of infectious bursal disease in poultry birds

“…[ 16 ] showed that the proper time for administration of the vaccine is 18 days post hatch with the management conditions in place at the farm. As this disease has been focused in recent reviews regarding its causative agent [ 17 ] and the vaccination [ 18 , 19 ], the present study is, therefore, mainly inclined toward the timely and reliable diagnosis of this threatening disease using gross, histopathology (HP), immunohistochemistry (IHC), and immunofluorescent methods.…”