Growth and protein profile changes in Lepidium sativum L. plantlets exposed to cadmium

Gianazza, Elisabetta; Wait, Robin; Sozzi, Andrea; Regondi, Simona; Saco, D.; Labra, Massimo; Agradi, E.

doi:10.1016/j.envexpbot.2005.12.005

Cited by 40 publications

(21 citation statements)

References 49 publications

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“…Alongside heat shock proteins, the abundance of peroxiredoxin and storage proteins fragments increased (Supporting Information Table S1), whereas the abundance of almost all of the proteins involved in energy metabolism decreased (Supporting Information Table S1), most likely because it is no longer necessary to keep up the full set of metabolic pathways. In accordance with this, an investigation of the cress seedling proteome by Gianazza et al 31 identified mostly storage proteins in the leftovers of the seeds still stuck to the plantlets (encompassing the endosperm and testa). In this context it is also interesting to note the strong drop in the accumulation level of a key enzyme of the “activated methyl cycle”, S ‐adenosyl‐ L ‐homocysteine hydrolase (spot no.…”

Section: Resultsmentioning

confidence: 67%

Proteomics reveal tissue‐specific features of the cress (Lepidium sativum L.) endosperm cap proteome and its hormone‐induced changes during seed germination

Müller

Job

Belghazi³

et al. 2010

Proteomics

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Mature angiosperm seeds consist of an embryo surrounded by the endosperm and the testa. The endosperm cap that covers the radicle plays a regulatory role during germination and is a major target of abscisic acid-induced inhibition of germination. Cress (Lepidium sativum) is a close relative of the model plant Arabidopsis thaliana (Arabidopsis). Cress seeds offer the unique possibility of performing tissue-specific proteomics due to their larger size while benefiting the genomic tools available for Arabidopsis. This work provides the first description of endosperm cap proteomics during seed germination. An analysis of the proteome of the cress endosperm cap at key stages during germination and after radicle protrusion in the presence and absence of abscisic acid led to the identification of 144 proteins, which were clustered by the changes in their abundances and categorized by function. Proteins with a function in energy production, protein stability and stress response were overrepresented among the identified endosperm cap proteins. This strongly suggests that the cress endosperm cap is not a storage tissue as the cereal endosperm but a metabolically very active tissue regulating the rate of radicle protrusion.

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Section: Resultsmentioning

confidence: 67%

Proteomics reveal tissue‐specific features of the cress (Lepidium sativum L.) endosperm cap proteome and its hormone‐induced changes during seed germination

Müller

Job

Belghazi³

et al. 2010

Proteomics

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“…Similar to the present studies, increase in the levels of RNA and protein in both endosperms as well as embryo axes was also observed earlier in rice seeds germinated under high level of cadmium Dubey 1995, 1998). Proteomics studies of germinating Lepidium sativum seeds showed inhibition of both storage protein catabolism and plant protein anabolism in response to cadmium exposure (Gianazza et al 2007). In the present study, increased levels of RNA and soluble proteins in embryo axes of nickel treated germinating rice seeds could be either due to increased synthesis of stress-specific proteins and their corresponding RNAs or due to inhibition in their degradation due to decline in the activities of RNases and proteases under such conditions.…”

Section: Discussionmentioning

confidence: 98%

Inhibition of ribonuclease and protease activities in germinating rice seeds exposed to nickel

Maheshwari

Dubey

2008

Acta Physiol Plant

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When the seeds of two rice cvs. Malviya-36 and Pant-12 were germinated up to 120 h in the presence of 200 and 400 lM NiSO 4 , a significant reduction in the germination of seeds occurred. Seeds germinating in the presence of 400 lM NiSO 4 showed about 12-20% decline in germination percent, about 20-53% decline in lengths and about 8-34% decline in fresh weights of roots and shoots at 120 h of germination. Ni 2+ exposure of germinating seeds resulted in apparent increased levels of RNA, soluble proteins, and free amino acids in endosperms as well as embryo axes. A 400 lM Ni 2+ treatment led to about 58-101% increase in the level of soluble proteins and about 39-107% increase in the level of free amino acids in embryo axes at 96 h of germination. Activities of ribonuclease and protease declined significantly with increasing levels of Ni 2+ treatment. Isoenzyme profile of RNase as revealed by activity staining indicated decline in the intensities of 3-4 preexisting enzyme isoforms in embryo axes of both the rice cultivars and disappearance of one of the two isoforms in endosperms of cv. Pant-12 due to 400 lM Ni 2+ treatment. Results suggest that the presence of high level of Ni 2+ in the medium of germinating rice seeds serves as a stress factor resulting in decreased hydrolysis as well as delayed mobilization of endospermic RNA and protein reserves and causing imbalance in the level of biomolecules like RNA, proteins, and amino acids in growing embryo axes. These events would ultimately contribute to decreased germination of rice seeds in high Ni 2+ containing environment.

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“…Cd is classified under the group I of human carcinogens which can lead to renal and lung cancer. 74 Cd arrests the plant growth and thus affects the biomass, 13,19,75 which can lead to plant death. 25 It interferes with photosynthesis, respiration and water relations 18,19,24,76 and the process of uptake and translocation of mineral nutrients lead to significant alterations in the normal plant growth.…”

Section: Cadmium Stress and Sulfur Metabolismmentioning

confidence: 99%

“…[4][5][6] In spite of its high phytotoxicity, Cd is easily taken up by plant roots and transported to above-ground tissues [7][8][9] and enters into the food chain where it may pose serious threats to human health. 10,11 The International Agency for Research on Cancer in 1993 12,13 classified Cd as a human carcinogen and, Plants can't move away and are therefore continuously confronted with unfavorable environmental conditions (such as soil salinity, drought, heat, cold, flooding and heavy metal contamination). Among heavy metals, cadmium (Cd) is a non-essential and toxic metal, rapidly taken up by roots and accumulated in various plant tissues which hamper the crop growth and productivity worldwide.…”

Section: Introductionmentioning

confidence: 99%

Cadmium stress tolerance in crop plants

Gill

Tuteja

2011

Plant Signaling & Behavior

340

137

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Growth and protein profile changes in Lepidium sativum L. plantlets exposed to cadmium

Cited by 40 publications

References 49 publications

Proteomics reveal tissue‐specific features of the cress (Lepidium sativum L.) endosperm cap proteome and its hormone‐induced changes during seed germination

Proteomics reveal tissue‐specific features of the cress (Lepidium sativum L.) endosperm cap proteome and its hormone‐induced changes during seed germination

Inhibition of ribonuclease and protease activities in germinating rice seeds exposed to nickel

Cadmium stress tolerance in crop plants

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