Growth and Photosynthesis Temperature Characteristics of the Sterile Ulva pertusa.

Murase, Noboru; Maegawa, Miyuki; Matsui, Toshio; Ohgai, Masaharu; Katayama, Nobuyasu; Saitoh, Munekatsu; Yokohama, Yasutsugu

doi:10.2331/suisan.60.625

Cited by 17 publications

(14 citation statements)

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“…Sterile U. pertusa thalli show optimal growth under a 12L/12D photoperiod at temperatures between 20 and 25°C in chamber cultivation, and can adapt to rapid temperature changes ranging from 5 to 30°C (Murase et al 1993;Kakinuma et al 2006). In a higher plant, Spinacia oleracea, the cytoplasmic Hsp70 genes display not only a thermal stress-induced expression pattern but also a diurnal expression pattern (Li et al 2000;Li and Guy 2001).…”

Section: Discussionmentioning

confidence: 99%

“…In fact, the consequences of some environmental stresses have been identified for these algae by laboratory experiments. In response to high-temperature and heavy metal stresses, these algae change their growth, morphology, chemical and enzymatic components, and photosynthetic activity (Murase et al 1993;Kakinuma et al 2001Kakinuma et al , 2006Han et al 2008). Thus, when exposed to changes in temperature and trace metals, the normal ''wild'' and the sterile U. pertusa appear to utilize a complex set of physiological and biochemical changes.…”

Section: Introductionmentioning

confidence: 96%

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Isolation and characterization of a cDNA encoding a heat shock protein 70 from a sterile mutant of Ulva pertusa (Ulvales, Chlorophyta)

et al. 2010

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Synthesis and accumulation of molecular chaperones are universal responses found in all cellular organisms when exposed to a variety of unfavorable conditions. Heat shock protein 70 (Hsp70), which is one of the major classes of molecular chaperones, plays a particularly important role in cellular stress responses, and the Hsp70 system is the most intensely studied in higher plants and algae. Therefore, we isolated and characterized a cDNA clone encoding Hsp70 from a sterile strain of Ulva pertusa (Ulvales, Chlorophyta). The sterile U. pertusa Hsp70 (UpHsp70) cDNA consisted of 2,272 nucleotides and had an open reading frame encoding a polypeptide of 663 amino acid (AA) residues with a molecular mass of 71.7 kDa. Amino acid alignment and phylogenetic analysis of Hsp70s from other organisms showed that UpHsp70 was more similar to cytoplasmic Hsp70s from green algae and higher plants (> or =75%) than to those from other algae and microorganisms. Southern blot analysis indicated that the sterile U. pertusa genome had at least four cytoplasmic Hsp70-encoding genes. UpHsp70 mRNA levels were significantly affected by diurnal changes, rapidly increased by high-temperature stress, and gradually increased by exposure to copper, cadmium, and lead. These results suggest that UpHsp70 plays particularly important roles in adaptation to high-temperature conditions and diurnal changes, and is potentially involved in tolerance to heavy metal toxicity.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Isolation and characterization of a cDNA encoding a heat shock protein 70 from a sterile mutant of Ulva pertusa (Ulvales, Chlorophyta)

et al. 2010

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“…in our study, U. armoricana was described as a new species in France (Dion et al, 1998) and is considered to have been introduced to Japan (Shimada et al, 2003). European species of Ulva have a relatively lower optimal temperature (10-20°C) for growth and photosynthesis than Japanese species (20-30°C) (Murase et al, 1993;De Casabianca & Posada, 1998;Taylor et al, 2001), and exhibit the highest growth or peak in biomass in spring to early summer (Sfriso, 1995;Viaroli et al, 1996b;De Casabianca & Posada, 1998;Naldi & Viaroli, 2002). These ecophysiological and seasonal characteristics do not correspond with those of Ulva spp.…”

Section: Discussionmentioning

confidence: 69%

Persistent occurrence of floating Ulva green tide in Hiroshima Bay, Japan: seasonal succession and growth patterns of Ulva pertusa and Ulva spp. (Chlorophyta, Ulvales)

Yoshida

Uchimura²,

Hiraoka

2015

Hydrobiologia

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Since the late 1980's, a persistent green tide of floating Ulva without any clear seasonal fluctuation has occurred in Hiroshima Bay, Seto Inland Sea, Japan. We hypothesized that the persistence is due to the co-existence of Ulva species with different seasonal growth patterns, and monitored the seasonal composition and growth characteristics of the constituent Ulva within the green tide. Two morphological types of Ulva were identified, and one type, U. pertusa, was almost the sole constituent during winter and spring. The other type Ulva spp., which has marginal microscopic serrations on the thallus, was dominant during summer and autumn. Both Ulva showed the highest relative growth rate in early autumn, but growth of Ulva spp. was faster in summer than that of U. pertusa and inhibited in winter. U.pertusa had more eurythermal characteristics in which the growth rate remained relatively high in winter. Water temperature was the most correlated environmental variable for the seasonal growth of both Ulva types rather than light or nutrients, but more influential on Ulva spp. Recent increasing trend of ambient seawater temperature is considered to be favorable for the growth of both Ulva types and a causative factor of the green tide.

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“…It is thought that the higher growth rate of sterile U. pertusa is mainly related to its high photosynthetic and low respiratory rates [6]. The initial carboxylation reaction in the photosynthetic carbon reduction cycle as well as in the initial oxygenation reaction in the photorespiratory carbon oxidation cycle in photosynthetic eukaryotes are catalyzed by a bifunctional enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) in chloroplast [9].…”

Section: Introductionmentioning

confidence: 99%

“…As compared with the ''wild'' strain, a sterile mutant of U. pertusa is easily maintained in the laboratory, since the alga only develops vegetatively under chamber cultivation [5]. Moreover, the alga grows rapidly and efficiently assimilates inorganic nitrogen sources [6]. Because of the advantage of the biological, physiological, and ecological characteristics of sterile U. pertusa, the alga has been used to reduce environmental eutrophication in coastal mariculture farms and as feed for the cultivation of juvenile fishes [7,8].…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of the rbcS genes from a sterile mutant of Ulva pertusa (Ulvales, Chlorophyta) and transient gene expression using the rbcS gene promoter

et al. 2009

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We isolated two different genomic DNAs (UprbcS1 and UprbcS2) encoding the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase and portions of the 5 0 -and 3 0 -flanking regions from sterile Ulva pertusa Kjellman. The UprbcS1 and UprbcS2 genes had three introns in the coding region. Each predicted UprbcS polypeptide was a 180-amino-acid (AA) residue including a 38-AA transit peptide, although the 104th AA residue was replaced. The nucleotide sequences of UprbcS cDNAs isolated from a cDNA library corresponded to that of the UprbcS1 gene, suggesting that the UprbcS1 gene was predominantly expressed in sterile U. pertusa compared to UprbcS2. Southern blot analysis showed that each UprbcS gene was a single-copy gene in the sterile U. pertusa genome. Northern hybridization indicated that the expression of UprbcS was induced and repressed by dark and light treatments, respectively. When sterile U. pertusa cells were transformed with an expression vector containing the UprbcS1 promoter and terminator sequences fused with the green fluorescent protein (GFP) gene, GFP fluorescence was observed in the cells transformed. These results suggest that the UprbcS1 gene promoter is light regulated and highly active in the sterile U. pertusa cells and is available for genetic transformation system in the alga.

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Growth and Photosynthesis Temperature Characteristics of the Sterile Ulva pertusa.

Cited by 17 publications

References 0 publications

Isolation and characterization of a cDNA encoding a heat shock protein 70 from a sterile mutant of Ulva pertusa (Ulvales, Chlorophyta)

Isolation and characterization of a cDNA encoding a heat shock protein 70 from a sterile mutant of Ulva pertusa (Ulvales, Chlorophyta)

Persistent occurrence of floating Ulva green tide in Hiroshima Bay, Japan: seasonal succession and growth patterns of Ulva pertusa and Ulva spp. (Chlorophyta, Ulvales)

Isolation and characterization of the rbcS genes from a sterile mutant of Ulva pertusa (Ulvales, Chlorophyta) and transient gene expression using the rbcS gene promoter

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