Growth and partial differentiation of presumptive human cardiac myoblasts in culture.

Kohtz, D. Stave; Dische, N R; Inagami, Tadashi; Goldman, Bruce

doi:10.1083/jcb.108.3.1067

Cited by 31 publications

(6 citation statements)

References 49 publications

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“…At present, GATA-6 has been linked to cardioblast proliferation whereas GATA-4 has been associated with terminal differentiation (12,15). In this respect, it is noteworthy that markers of later stages of cardiomyocyte differentiation-␣-and ␤-MHC genes-appear to be preferential GATA-4 targets whereas genes expressed in precardiomyocytes prior to the beating stage, such the natriuretic peptide (ANF and BNP) and PDGFR genes (16,24), are targeted by both GATA-4 and GATA-6. Since the adenovirus-mediated antisense strategy revealed that GATA-4 and GATA-6 are required for maintenance of the differentiated phenotype in postnatal cardiomyocytes, it could now be used to determine the role of these factors in embryonic myocytes.…”

Section: Discussionmentioning

confidence: 96%

Cooperative Interaction between GATA-4 and GATA-6 Regulates Myocardial Gene Expression

Charron

Paradis

Bronchain

et al. 1999

Molecular and Cellular Biology

222

196

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Two members of the GATA family of transcription factors, GATA-4 and GATA-6, are expressed in the developing and postnatal myocardium and are equally potent transactivators of several cardiac promoters. However, several in vitro and in vivo lines of evidence suggest distinct roles for the two factors in the heart. Since identification of the endogenous downstream targets of GATA factors would greatly help to elucidate their exact functions, we have developed an adenovirus-mediated antisense strategy to specifically inhibit GATA-4 and GATA-6 protein production in postnatal cardiomyocytes. Expression of several endogenous cardiac genes was significantly down-regulated in cells lacking GATA-4 or GATA-6, indicating that these factors are required for the maintenance of the cardiac genetic program. Interestingly, transcription of some genes like the alpha- and beta-myosin heavy-chain (alpha- and beta-MHC) genes was preferentially regulated by GATA-4 due, in part, to higher affinity of GATA-4 for their promoter GATA element. However, transcription of several other genes, including the atrial natriuretic factor and B-type natriuretic peptide (ANF and BNP) genes, was similarly down-regulated in cardiomyocytes lacking one or both GATA factors, suggesting that GATA-4 and GATA-6 could act through the same transcriptional pathway. Consistent with this, GATA-4 and GATA-6 were found to colocalize in postnatal cardiomyocytes and to interact functionally and physically to provide cooperative activation of the ANF and BNP promoters. The results identify for the first time bona fide in vivo targets for GATA-4 and GATA-6 in the myocardium. The data also show that GATA factors act in concert to regulate distinct subsets of genes, suggesting that combinatorial interactions among GATA factors may differentially control various cellular processes.

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Section: Discussionmentioning

confidence: 96%

Cooperative Interaction between GATA-4 and GATA-6 Regulates Myocardial Gene Expression

Charron

Paradis

Bronchain

et al. 1999

Molecular and Cellular Biology

222

196

View full text Add to dashboard Cite

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“…Cytokinesis can also occur in embryonic, neonatal, and some adult heart muscle cells under appropriate conditions [Chacko, 1973;Goode, 1975;Kohtz et al, 1989;Kaneko et al, 1984;Oberpriller et al, 1988;Tate and Oberpriller, 19891. Contractions cease in the area of the cleavage furrow [Kaneko et al, 19841, and myofibrils are disassembled [Goode, 19751. When cytokinesis is completed, daughter cells reassemble myofibrils and resume rhythmic contractions [Kaneko et al, 1984;Chacko, 19731.…”

Section: Introductionmentioning

confidence: 99%

Subcellular compartmentalization of myosin isoforms in embryonic chick heart ventricle myocytes during cytokinesis

Conrad

Clark

Conrad

1991

Cell Motil. Cytoskeleton

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Embryonic chick heart ventricle myocytes retain the ability to alternate between proliferation and functional differentiation. A cytoplasmic isoform of myosin is present in cleavage furrows of various nonmuscle cells during cytokinesis, whereas one or more of the cardiac myosin isoforms are localized in sarcomeres of beating cardiomyocytes. Antibodies were employed to reveal the subcellular localizations of cytoplasmic and cardiac myosin isoforms in embryonic chick ventricle cardiomyocytes during cytokinesis. Monoclonal anticytoplasmic myosin antibodies were prepared against myosin purified from brains of 1-day-posthatched chickens and shown to react with chick brain myosin heavy chain by Western blots and/or ELISA tests. One monoclonal antibrain myosin antibody also cross-reacted with chick cardiac myosin but not with skeletal or smooth muscle myosins. Two antichick cardiac myosin monoclonal antibodies and one antichick skeletal myosin polyclonal antibody that cross-reacts with cardiac myosin were employed to identify cardiac sarcomeric myosin. Cells were isolated from day 8 embryonic chick heart ventricles, enriched for myocytes, grown in vitro for 3 days, and then examined by immunofluorescence techniques. Monoclonal antibodies against cytoplasmic myosin preferentially localized in the cleavage furrows of both cardiofibroblasts and cardiomyocytes in all stages of cytokinesis. In contrast, antibodies that recognize cardiac myosin were distributed throughout cardiomyocytes during early stages of cytokinesis, but became progressively excluded from the furrow area during middle and late stages of cytokinesis. These data suggest that in cells that contain both cytoplasmic and sarcomeric myosin isoforms, only cytoplasmic myosin isoforms are mobilized to from the contractile ring for cytokinesis.

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“…The expression of the ANP gene has been used as a marker for the differentiation of atrial and ventricular myocytes, both in the developing heart in situ and in culture (Gilloteaux 1989;Kohtz et al 1989). As in the human heart in situ (Hamid et al 1987;Wharton et al 1988a, b;Yamada et al 1988), cultured fetal atrial and ventricular myocytes were ANP-like immunoreactive.…”

Section: Discussionmentioning

confidence: 97%

“…The mixed cell culture preparations described here may be a useful model for the direct study of the factors that influence ANP expression in human myocytes. It has already been reported that neuroblastoma cells in co-culture may increase the stability of ANP mRNA in human fetal ventricular myocytes (Kohtz et al 1989), and the presence of intracardiac neurones in the cultures from human fetal atria described would maintain any effect of the intrinsic neurones that innervate the heart in situ on ANP.…”

Section: Discussionmentioning

confidence: 98%

Immunocytochemical studies of cardiac myocytes and other non-neuronal cells of the fetal human heart in culture

et al. 1990

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Non-neuronal cell types present in cultures dissociated from the atria and ventricles of human fetal hearts, from 9 to 21 weeks' gestation, were studied using phase-contrast optics and immunocytochemistry. All atrial myocytes and many, if not all, ventricular myocytes observed in culture contained atrial natriuretic peptide-like immunoreactivity. Generally, the atrial natriuretic peptide-like immunoreaction in atrial myocytes was more intense and widespread than in ventricular myocytes. Atrial and ventricular fibroblasts expressed extracellular fibronectin-like immunoreactivity. A population of cells with the appearance and growth properties of endothelial cells was observed in both atrial and ventricular cultures, and was classified as endothelioid since their precise origin was not known. Only a subpopulation of these endothelioid cells contained factor VIII-related antigen immunoreactivity, and some cells that did not display the other growth characteristics of endothelial cells were also factor VIII-related antigen immunoreactive in culture. Glial cells were S-100-like immunoreactive; they were usually more numerous in atrial than ventricular preparations. There was no close association between glial cells and neurones in the atrial cultures.

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Growth and partial differentiation of presumptive human cardiac myoblasts in culture.

Cited by 31 publications

References 49 publications

Cooperative Interaction between GATA-4 and GATA-6 Regulates Myocardial Gene Expression

Cooperative Interaction between GATA-4 and GATA-6 Regulates Myocardial Gene Expression

Subcellular compartmentalization of myosin isoforms in embryonic chick heart ventricle myocytes during cytokinesis

Immunocytochemical studies of cardiac myocytes and other non-neuronal cells of the fetal human heart in culture

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