Growth and Oleanolic Acid Accumulation of Polyscias fruticosa Cell Suspension Cultures

Abstract

Background: Oleanolic acid is an oleanane triterpene found in many plant species all over the world. This compound is also a major saponin in leaves of Polyscias fruticosa and possess several promising pharmacological activities, such as hepatoprotective effects, and anti-inflammatory, antioxidant, or anticancer activities. Objective: The objective of the present work is to establish cell suspension culture of P. fruticosa, investigate influence of several factors such as plant growth regulators and carbon source on cell growth, and determine their oleanolic acid content. Method: Cell culture was carried out by using 2 g fresh weight of 30 day old friable callus derived from in vitro stem segment in 50 mL of liquid medium with a shaking speed of 120 rpm. Culture was then incubated at 25±2ºC with a shaking speed of 120 rpm in the period of 12 h daylight at light intensity of about 6.75 µmol/m2 /s. Cell growth was measured by fresh and dry biomass at 16h day. Oleanolic acid content was determined using HPLC analysis. Results & Discussion: The study results showed that MS medium containing 2% sucrose as a carbon source, supplemented with 1 mg/L 6-benzylaminopurine and 0.5 mg/L 2,4-D dichlorophenoxyacetic acid was the most appropriate growth medium. Cell biomass and oleanolic acid content reached the highest values of 0.43 g dry weight/flask and 25.4 mg/g dry weight, respectively. Conclusion: These results indicated the potential production of oleanolic acid, a compound with high pharmacological value, from P. fruticosa cell culture.

Suspension Cell Culture of Polyscias fruticosa (L.) Harms in Bubble-Type Bioreactors—Growth Characteristics, Triterpene Glycosides Accumulation and Biological Activity

“…Our results demonstrated that the suspension cell culture of P. fruticosa grew well in flasks (Table 1). The maximum dry weight accumulation in flasks achieved in our study (8.2 g L −1 at the inoculum density 1.1 g L −1 ) was similar to what was previously recorded for the same cell strain [40,44] and to those reported for the suspension cell cultures of P. fruticosa by Kim et al [31] (0.33-0.45 g per flask or 6.5-9.0 g L −1 depending on the media composition at inoculum density 3.3-3.4 g L −1 ). The cell culture showed a lag phase when subcultured with inoculum density about 1.0 g L −1 [44].…”

Antifungal Peptide P852 Controls Fusarium Wilt in Faba Bean (Viciafaba L.) by Promoting Antioxidant Defense and Isoquinoline Alkaloid, Betaine, and Arginine Biosyntheses

“…In contrast to H treatment, the levels of organic acids, especially oleanolic acid and 2-hydroxycinnamic acid, were barely increased under D treatment, relative to CK ( Figure 7 C). It was found that numerous plant growth regulators and carbon sources could alter oleanolic acid levels in Polyscias fruticosa pericarp tissue suspension culture [ 62 ], and it is generally recognized to be present ubiquitously in plants participating in plant defenses and defense systems against pathogens [ 63 , 64 ]. The contents of other organic acid, including benzoic acid, critic acid, caffeic acid, malic acid, and gluconic acid were all elevated under the H treatment.…”