Growth and Neutralization of the Trachoma Agent In Mouse Lungs

Graham, Doris M.

doi:10.1038/2071379a0

Cited by 17 publications

(7 citation statements)

References 14 publications

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“…The data shown are mean values from three separate experiments. neutralization was described by using either antisera against whole organisms (1,13,20,31) or secretions from chlamydia-infected tissues (2,12,27). Neither the antibody specificity involved in neutralization nor the mechanism by which neutralization occurs was studied by those investigators.…”

Section: Discussionmentioning

confidence: 99%

Neutralization of Chlamydia trachomatis infectivity with antibodies to the major outer membrane protein

Caldwell¹,

Perry²

1982

Infect Immun

133

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Rabbit immunoglobulin G (IgG) antibodies raised against the major outer membrane protein of the Chlamydia trachomatis lymphogranuloma venereum strain 434 neutralized the infectivity of the parasite for HeLa 229 cells. The mechanism by which anti-major outer membrane protein IgG prevented C. trachomatis from establishing infection was studied by using intrinsically "4Cradiolabeled elementary bodies. Neutralized elementary bodies were filterable through a polycarbonate filter (pore diameter, 600 nm), demonstrating that reduction in infectivity was not due to the aggregation of elementary bodies by cross-linking IgG. Antibody-neutralized elementary bodies attached to and penetrated HeLa cells at rates nearly identical to those for infectious organisms exposed to nonneutralizing control IgG. These results suggest that antibody interferes with the infectious process of the parasite after its internalization. Antimajor outer membrane protein Fab fragments could not be substituted for neutralizing IgG antibodies. The requirement for intact IgG implies that crosslinking of antibodies to the major outer membrane protein on the surfaces of the organisms may be instrumental in neutralization.

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Section: Discussionmentioning

confidence: 99%

Neutralization of Chlamydia trachomatis infectivity with antibodies to the major outer membrane protein

Caldwell¹,

Perry²

1982

Infect Immun

133

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“…By this criterion, and from examinations for inclusion bodies, they concluded that tt One animal had a significantly high score (67). (Graham, 1965) suggest that the 'fast-killing' variant strains differ from their parent 'slow-killing' strains in the degree of crossprotection they give against pulmonary infection of mice; nevertheless, the 'fastkilling' strain MRC-4f used for immunization in Expt. 3 induced immunity against its parent strain in two of five monkeys receiving undiluted vaccine.…”

Section: Discussionmentioning

confidence: 99%

Immunogenicity of experimental trachoma vaccines in baboons: I. Experimental methods, and preliminary tests with vaccines prepared in chick embryos and in HeLa cells

Collier

Blyth

1966

Epidemiol. Infect.

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Parallel titrations of a strain of trachoma (MRC–221) and one of inclusion conjunctivitis (MRC–4) in the baboon conjunctiva and in chick embryos suggest that ten to twenty 50% egg infective doses are equivalent to one 50% baboon infective dose; but that at least 1000 egg infective doses are needed to induce moderate or severe infections in all of a given number of baboons.For vaccine experiments in baboons, a system of scoring physical signs and presence of inclusion bodies was devised; the significance of differences in vaccinated and control animals in their response to conjunctival challenge was determined by analysis of variance. An aqueous suspension of live MRC–4 grown in the yolk sac was given as two subcutaneous doses and one intravenous dose at weekly intervals, and protected all of six baboons challenged with the homologous strain; three similarly spaced subcutaneous doses were less effective. The immunity induced by this vaccine waned considerably during the ensuing 15 months. Vaccine prepared from a live ‘fast-killing’ variant of MRC–4 grown in HeLa cells was less effective than MRC–4 itself in protecting baboons against infection with the parent strain.Although both yolk sac and HeLa cell vaccines induced the formation of antibody fixing complement with trachoma group antigen, the serum titres in individual animals at the time of challenge were unrelated to the degree of protection; during a 15 month observation period there were pronounced falls in the titres of antibody induced either by vaccination or by challenge with egg-grown TRIC agent.We wish to thank Dr I. Sutherland (M.R.C. Statistical Research Unit) for his helpful advice during the early stages of this work. We are also greatly indebted to Mr P. Avis (Pfizer Ltd.) for his advice and for undertaking the statistical computations; and to Miss Anne Smith and Miss Pay Storey (M.R.C. Trachoma Research Unit) for doing the complement fixation tests.

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“…It is known that some strains of C. trachomatis infect mouse lungs [7] and that the so-called "fast- Table 2 Occurrence of infection in progesterone-treated CBA mice of different ages inoculated by two different routes with strain SA2(f ) nt, not tested. taa killing" strains multiply more rapidly than do the "slow-killing" strains [8].…”

Section: Inoculation Of Mice With Strain Sac(f) In the Absence Of Hormentioning

confidence: 99%

Progesterone as a key factor in the development of a mouse model for genital-tract infection with Chlamydia trachomatis

Tuffrey

1981

FEMS Microbiology Letters

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Growth and Neutralization of the Trachoma Agent In Mouse Lungs

Cited by 17 publications

References 14 publications

Neutralization of Chlamydia trachomatis infectivity with antibodies to the major outer membrane protein

Neutralization of Chlamydia trachomatis infectivity with antibodies to the major outer membrane protein

Immunogenicity of experimental trachoma vaccines in baboons: I. Experimental methods, and preliminary tests with vaccines prepared in chick embryos and in HeLa cells

Progesterone as a key factor in the development of a mouse model for genital-tract infection with Chlamydia trachomatis

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