Growth and mycelial strand production of Rigidoporus lignosus with various nitrogen and carbon sources

Abstract

Growth and differentiation of mycelial strands in Rigidoporus lignosus have been shown to depend on suitable combinations of the pH of the media and the nature of the nitrogen and carbon sources. Amino acids as sole nitrogen sources gave rise to vegetative mycelium. At pH 4.5, growth and mycelial strand differentiation required asparagine, as the fungus failed to grow in the absence of this amino acid. However, at pH 6, differentiation of strands occurred appreciably in asparagine-deficient media, suggesting a close balance between pH and amino acid requirements. Ammonium was required for strand differentiation, while nitrate, as a sole nitrogen source, maintained the fungus undifferentiated. Of the carbohydrates tested, only glucose, fructose and mannose supported strand differentiation. Starch was found to be particularly effective in promoting growth of vegetative mycelium. Strand differentiation required more specific conditions than growth of the vegetative mycelium.

Silicon mediates the changes in physiological performance, nutrient uptake, root colonization morphology and secondary metabolite activity in rubber rootstock seedlings (Hevea brasiliensis) inoculated with Rigidoporus microporus

“…Scanning electron microscopy of uninoculated root tissue showed a higher starch accumulation than on inoculated roots of rubber rootstock seedlings. Starch is the most effective carbohydrate in promoting mycelial growth (Richard & Botton, 1996). Pathogens reallocate plant sugars for their own needs during infection, forcing plants to modify their sugar content and activating defence responses (Tauzin & Giardina, 2014).…”

Swarms, swarming and entanglements of fungal hyphae and of plant roots

“…42 Mycelial-cord formation is favored by the presence of localized sources of nutrients and modulated by long-distance transport of stimuli from other regions of the mycelium, 43 as well as by specific nutritional conditions present in the substrate or growth medium. 44 Snider 45 documented the formation of mycelial cords in laboratory cultures of the basidiomycete, Armillaria mellea .…”