Growth and Morphogenesis of Asparagus Cells cultured in vitro

Wilmar, Cecile; Hellendoorn, Marja

doi:10.1038/217369a0

Cited by 98 publications

(20 citation statements)

References 4 publications

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“…Asparagus officinalis L. was one of the first monocot species observed to regenerate somatic embryos through suspension cultures (Steward & Mapes 1971;Wilmar & Hellendoorn 1968). Wilmar & Hellendoorn (1968) found that hypocotylderived callus formed a large number of globular embryos when placed on solidified Linsmaier & Skoog (1965) medium (LS) containing 0.45 IxM 2,4-D and 1.41~M kinetin.…”

Section: Introductionmentioning

confidence: 99%

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Asparagus somatic embryos: Production in suspension culture and conversion to plantlets on solidified medium as influenced by carbohydrate regime

Levi

Sink

1992

Plant Cell Tiss Organ Cult

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Methods were developed for the production of somatic embryos of asparagus (Asparagus officinalis L.) in suspension culture and subsequent conversion to plantlets on solidified medium. Stem-derived callus that was subcultured twice on Murashige and Skoog (MS) medium + 0.54 IxM naphthaleneacetic acid (NAA) and 1.4 IxM 2-isopentenyladenine (2iP) was used to initiate suspension cultures. Six out of 15 such cell suspensions (MS medium with 54 to 107 IxM NAA) had a high embryogenic capacity. These cell suspensions consisted primarily of single elongated cells (about 90% of all single cells), embryogenic cell clusters (2571/ml), and globular translucent embryos (32/ml). The latter converted to plantlets within four weeks on embryo development medium (EDM), which was solidified MS medium containing 0.54 IxM NAA and 0.98 IxM 2iP. Suspension-derived embryos formed secondary globular embryos at high frequencies (251 to 258/g callus) when placed on EDM with a low carbohydrate (sucrose, glucose or fructose) level (2%). In contrast, EDM with a high carbohydrate level (10%) caused a reduction in the frequency of secondary embryos (30 to 85/g callus), while resulting in the promotion of embryo growth and conversion, 3.6 to 8.5 times higher than 2% carbohydrates. Transfer of globular somatic embryos from cell suspension to EDM with high carbohydrate levels (4 to 10%) for two weeks followed by transfer to EDM with a low carbohydrate level (2%) resulted in a 2 to 4 times higher conversion rate to plantlets than those that remained at the 4 to 10% levels.Abbreviations: ANOVA-analysis of variance, EDM-embryo development medium, NAAnaphthaleneacetic acid, MS-Murashige and Skoog, RCBD-randomized complete block design, 2iP-2-isopentenyladenine, 2,4-0-2,4-dichlorophenoxyacetic acid, IAA-indoleacetic acid

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Section: Introductionmentioning

confidence: 99%

“…Wilmar & Hellendoorn (1968) found that hypocotylderived callus formed a large number of globular embryos when placed on solidified Linsmaier & Skoog (1965) medium (LS) containing 0.45 IxM 2,4-D and 1.41~M kinetin. When placed in LS medium in suspension, the embryos converted to plantlets.…”

Section: Introductionmentioning

confidence: 99%

Asparagus somatic embryos: Production in suspension culture and conversion to plantlets on solidified medium as influenced by carbohydrate regime

Levi

Sink

1992

Plant Cell Tiss Organ Cult

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“…Asparagus is the first monocotyledonus species which showed regeneration of plantlets in vitro through somatic embryogenesis, and the embryogenic callus culture was first produced from the hypocotyls of sterile seedlings [5]. Since then, many studies have been conducted on the culture requirements for somatic embryogenesis, and asparagus has been chosen as one of the model plants in many laboratories.…”

Section: Introductionmentioning

confidence: 99%

Somatic Embryogenesis and Its Application for Breeding and Micropropagation in Asparagus (Asparagus officinalis L.).

Kunitake¹,

Mii

1998

Plant Biotechnology

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“…Successful culturing of callus has recently been reported for the following monocotyledonous species: corn (Reference 2, and Linsmaier Bednar, E. M., personal communication), oats (1,6), rice root (8), asparagus (7), wheat (5), and sorghum (4).…”

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confidence: 99%