Growth and metabolism of dark septate endophytes and their stimulatory effects on plant growth

Wang, Shuhui; Bi, Yinli; Quan, Wenzhi; Christie, Peter

doi:10.1016/j.funbio.2022.08.006

Cited by 12 publications

(16 citation statements)

References 62 publications

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“…Moreover, studies indicate that the extracellular metabolites of DSE are also closely related to the growth and tolerance of plants (Wang et al, 2022). Subsequent investigations demonstrate that DSE colonization can modulate plant tolerance by modifying cellular metabolism, F I U R E 4 Relative contributions of osmotica, antioxidant enzymes, antioxidants, and polyamines to the MDA.…”

Section: Discussionmentioning

confidence: 99%

“…The colonization of host plants by DSE assists plants in the uptake of nutrients in nutrient‐deficient soils and increases the tolerance of plants in stressful environments (Vergara et al, 2017). Moreover, studies indicate that the extracellular metabolites of DSE are also closely related to the growth and tolerance of plants (Wang et al, 2022). Subsequent investigations demonstrate that DSE colonization can modulate plant tolerance by modifying cellular metabolism, cascading signal transduction and triggering the synthesis of plant hormones and metabolites (Wu et al, 2020, 2021).…”

Section: Discussionmentioning

confidence: 99%

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Dark septate endophyte inoculation enhances antioxidant activity in Astragalus membranaceus var. mongholicus under heat stress

Bi,

Xue

2023

Physiologia Plantarum

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The influence of dark septate endophytic (DSE) on the antioxidant activity of Astragalus membranaceus var. mongholicus under heat stress was investigated. A. membranaceus plants, with or without DSE inoculation, were grown at 28°C for 8 weeks in a greenhouse and subsequently subjected to heat stress conditions (42°C) in an artificial climate chamber. DSE inoculation significantly decreased the malondialdehyde (MDA) content during the initial three days of heat stress. The activities of superoxide dismutase (SOD) and peroxidase (POD) of A. membranaceus leaves were significantly enhanced by DSE inoculation under heat stress, with SOD activities being 63–81% higher than in other treatments. The glutathione (GSH) and putrescine (Put) contents accumulated significantly on the third day under heat stress with DSE inoculation. Additionally, the contents of soluble sugars and proline (Pro) exhibited significant increases on the seventh day of heat stress and were 33–55% and 81–83% higher than in other treatments, respectively. Three‐way ANOVA shows that DSE inoculation under heat stress exerted a significant impact on MDA. Multivariate linear regression and structural equality modelling (SEM) further show that the interaction among these antioxidants significantly decreased MDA content and maintained the normal function of cell membranes. In conclusion, DSE inoculation enhanced the heat tolerance of A. membranaceus by boosting its antioxidant capacity and reducing MDA production. This study highlights the potential of utilizing DSE as a strategy to enhance plant heat tolerance.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Dark septate endophyte inoculation enhances antioxidant activity in Astragalus membranaceus var. mongholicus under heat stress

Bi,

Xue

2023

Physiologia Plantarum

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“…Just like loline, N2-fructopyranosylargenine is known to be produced by symbiotic fungal endophytes. The genus Alternaria has been shown to excrete substantial amounts of this compound to promote the growth of its host plants (Wang et al, 2022). Given that Cakile is well known to associate with fungi of the genus Alternaria (Chalbi et al, 2021), our findings are indicative of a higher abundance and more severe heat-stress induced disruption of such beneficial interactions in plants from Northern European origins.…”

Section: Discussionmentioning

confidence: 99%

A metabolomic view on local climate adaptation: Latitudinal divergence of heat and drought responses in a coastal plant

Schrieber

Gluesing

Peters

et al. 2022

Preprint

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Studying natural variation in multi-stress resistance is central for predicting and managing the population dynamics of wild plant species under rapid global change. Yet, it remains a challenging goal in this field to integrate knowledge on the complex biochemical underpinnings for the targeted non-model species. Here, we studied latitudinal divergence in combined drought and heat stress resistance in European populations of the dune plant Cakile maritima, by combining comprehensive plant phenotyping with metabolic profiling via FT-ICR-MS and UPLC-TQ-MS/MS. We observed pronounced constitutive divergence in growth phenology, leaf functional traits and defence chemistry (glucosinolates, alkaloids) among population origins. Most importantly, the magnitude of growth reduction under stress was partly weaker in southern plants and associated with divergence in plastic growth responses (root expansion, leaf abscission) and the stress-induced modulation of primary and specialized metabolites with known central functions not only in plant abiotic but also biotic stress resistance. Our study supports that divergent selection has shaped the constitutive and stress-induced expression of numerous morphological and biochemical functional traits to mediate higher abiotic stress resistance in southern Cakile populations, and highlights that metabolomics is a powerful tool to explore the mechanistic underpinnings of local stress adaptation in non-model species.

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“…Two Petri dishes with a diameter of 7 mm were used to inoculate 150 mL Modified Melin-Norkra (MMN) medium under sterile conditions. The DSE fermentation broth was harvested after culturing with shaking (170 rpm) at 27 C in the dark for 8 days (Wang et al, 2022b). The DSE fermentation culture was divided into 10 mL centrifuge tubes and centrifuged at 5550 Â g and 4 C for 15 min to obtain DSE mycelia and extracellular metabolites.…”

Section: Preparation Of Dse Mycelium and Extracellular Metabolitesmentioning

confidence: 99%

“…The beneficial effect of DSE on roots may be due to the promotion of plant hormone production (Priyadharsini & Muthukumar, 2017). Our previous studies indicate that the presence of indole substances in DSE extracellular metabolites can also promote plant root development (Wang et al, 2022b).…”

Section: F I G U R Ementioning

confidence: 99%

Effects of a dark septate endophyte and extracellular metabolites on alfalfa root exudates: a non‐targeted metabolomics analysis

Bi,

Wang,

Song

et al. 2024

Physiologia Plantarum

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Dark septate endophytes (DSE) are widespread colonizers of plant roots and have important ecological functions such as the regulation of plant growth and nutrient uptake. The mechanisms by which DSE mycelium and its extracellular metabolites promote plant growth have not yet been determined. Here, the growth‐promoting effects of DSE mycelium (H) and extracellular metabolites (M) on alfalfa (Medicago sativa L.) were investigated. Treatments H, M and HM increased the total biomass of alfalfa by 23.9%, 47.2% and 47.1%, respectively. H and M modified root structure by increasing root volume and reducing root tissue density, and promoting nutrient uptake. Metabolomic analysis indicates that alfalfa root exudates contained 204 metabolites of different types, mainly lipids and lipid‐like molecules, organic acids and derivatives, benzenoids. There were more organoheterocyclic compounds and fewer organic acids and derivatives in root exudates in treatment H than in treatment M. Pathway analysis shows that DSE and its extracellular metabolites had greater effects on glycerophospholipid metabolism and N‐glycan biosynthesis pathway. The results provide information on the mechanisms involved in the metabolic regulation of plant growth promotion by DSE.

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Growth and metabolism of dark septate endophytes and their stimulatory effects on plant growth

Cited by 12 publications

References 62 publications

Dark septate endophyte inoculation enhances antioxidant activity in Astragalus membranaceus var. mongholicus under heat stress

Dark septate endophyte inoculation enhances antioxidant activity in Astragalus membranaceus var. mongholicus under heat stress

A metabolomic view on local climate adaptation: Latitudinal divergence of heat and drought responses in a coastal plant

Effects of a dark septate endophyte and extracellular metabolites on alfalfa root exudates: a non‐targeted metabolomics analysis

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