Growth and Metabolic Responses of Rice (Oryza sativa L.) Cultivated in Phosphorus-Deficient Soil Amended with TiO₂ Nanoparticles

Abstract

Plants have the natural ability to withstand stress conditions through metabolic adjustments. The present study aimed at investigating the effects of titanium dioxide nanoparticles (TiO NPs) application (0, 25, 50, 150, 250, 500, and 750 mg kg) in phosphorus-deficient soil in terms of growth responses, P contents, and metabolic alterations in rice. TiO NPs application increased shoot length up to 14.5%. Phosphorus contents in rice roots, shoots, and grains were increased by 2.6-, 2.4-, and 1.3-fold, respectively, at 750 mg kg of TiO NPs. Gas chromatography-mass spectrometry (GC-MS)-based metabolomics revealed increased levels of amino acids, palmitic acid, and glycerol content in grains resulting from plants grown in 750 mg kg TiO NPs-treated soil. Furthermore, no translocation of TiO NPs from the treated soil to rice grains was detected by inductively coupled plasma-optical emission spectrometry (ICP-OES), which suggests no risk of TiO NPs intake via grain consumption. The observed data indicates the strong relationship among NPs application, P contents, and metabolic alterations.

Data on rhizosphere pH, phosphorus uptake and wheat growth responses upon TiO2 nanoparticles application

“…TiO 2 general purpose reagent was obtained from Sigma Aldrich Inc. (purity > 99%, St. Louis, MO, USA) and further processed, and calcined at 500 °C to synthesize pure anatase crystal structure of TiO 2 NPs as described in Zahra et al [1] . Scanning electron microscope (SEM, Jeol, JSM 6490A, Tokyo, Japan), energy-dispersive X-ray spectroscopy (EDX, Jeol, JED 2300), and X-ray diffraction (XRD) analyses of as-prepared TiO 2 NPs were performed as shown in Fig 1 .…”

Adaption and recovery of Nitrosomonas europaea to chronic TiO2 nanoparticle exposure

“…Due to their extensive application, TiO 2 NPs are inevitably released into the environment and are recognized as an emerging environmental stressor (Gondikas et al 2014). As a result, the negative impacts of TiO 2 NPs on animal cells (Yu et al 2017), aquatic organisms (Hu et al 2017), bacterial cells (Liu et al 2016), plants (Zahra et al 2017) , and biofilm communities (Binh et al 2016) have been widely recognized and have raised increasing concerns regarding their biosafety and biosecurity in ecosystems.…”

Characterization of TiO2 NPs in Radish (Raphanus sativus L.) by Single-Particle ICP-QQQ-MS

“…It should be also highlighted that plant cultivation with TiO 2 NPs influences accumulation of other elements. For example, the increasing content of P, Ca, Mg, and Zn was determined in roots, shoots, and grains of rice (Du et al, 2017;Zahra et al, 2017), whereas an enhanced accumulation of Fe in roots (Burke et al, 2015) was shown in the case of soybean. On the other hand, the differences in the physicochemical properties of TiO 2 NPs such as their size, aggregation, surface area, coating, and crystal structure have a huge influence on the interactions of NPs with living organisms like plants, inducing different toxicities (Du et al, 2019).…”