Growth and immune system performance to assess the effect of dispersed oil on juvenile sea bass (Dicentrarchus labrax)

Dussauze, Matthieu; Danion, Morgane; Floch, Stéphane Le; Lemaire, Philippe; Théron, Michaël; Pichavant-Rafini, Karine

doi:10.1016/j.ecoenv.2015.05.018

Cited by 19 publications

(9 citation statements)

References 50 publications

(57 reference statements)

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“…This result is in accordance with previous observations obtained in field operations and in situ experimentation [22,23]. Taken together and very logically, these results show that the The device presented here makes possible to measure the evolution of the total petroleum hydrocarbon concentration during this period and our results show no important reduction of TPH concentration over the 24 hours of contamination (Table 1).…”

Section: Resultssupporting

confidence: 82%

An Experimental System to Assess Potential Biological Impact of Operational Response During an Oil Spill

Dussauze¹,

Pichavant-Rafini²,

Théron³

et al. 2016

J Pet Environ Biotechnol

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In order to evaluate the potential toxicity of dispersant application in an oil slick in near-shore areas, this study presents an experimental system designed to perform toxicity tests on fish. Three possible oil spill scenario issues during an oil spill were tested on juvenile sea bass (Dicentrarchus labrax); the Water Soluble Fraction of oil; the Mechanical Dispersion of oil; the Chemical Dispersion of oil. Preliminary toxicity assays suggest that the experimental system is appropriate to assess the three experimental conditions during a period of 24 hours. This experimental system allowed obtaining "quick" and relevant results of acute toxicity in an emergency context such an oil spill.

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Section: Resultssupporting

confidence: 82%

An Experimental System to Assess Potential Biological Impact of Operational Response During an Oil Spill

Dussauze¹,

Pichavant-Rafini²,

Théron³

et al. 2016

J Pet Environ Biotechnol

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show abstract

“…anguillarum were negligible in No Oil/Pathogen challenged fish but comprised up to 75% of the lower and upper gills of Oil/Pathogen challenged fish indicating a immunocompromisation induced by oil exposure (3D). In several fish species, a reduced ability to defend against pathogen infection occurred after exposure to oil or its components [27,29,35,36,64,76,77]. Rainbow trout experienced a 10% higher mortality following a 50 dy dietary PAH exposure and disease challenge as compared to control fish [76].…”

Section: Resultsmentioning

confidence: 99%

“…Rainbow trout experienced a 10% higher mortality following a 50 dy dietary PAH exposure and disease challenge as compared to control fish [76]. Similarly, sea bass challenged with a Viral Nervous Necrosis Virus experienced elevated mortality when previously exposed to dispersed oil, however, there were no lasting impacts on the innate immunity [77]. In the congener species P .…”

Section: Resultsmentioning

confidence: 99%

Crude oil impairs immune function and increases susceptibility to pathogenic bacteria in southern flounder

et al. 2017

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Exposure to crude oil or its individual constituents can have detrimental impacts on fish species, including impairment of the immune response. Increased observations of skin lesions in northern Gulf of Mexico fish during the 2010 Deepwater Horizon oil spill indicated the possibility of oil-induced immunocompromisation resulting in bacterial or viral infection. This study used a full factorial design of oil exposure and bacterial challenge to examine how oil exposure impairs southern flounder (Paralichthys lethostigma) immune function and increases susceptibility to the bacteria Vibrio anguillarum, a causative agent of vibriosis. Fish exposed to oil prior to bacterial challenge exhibited 94.4% mortality within 48 hours of bacterial exposure. Flounder challenged with V. anguillarum without prior oil exposure had <10% mortality. Exposure resulted in taxonomically distinct gill and intestine bacterial communities. Mortality strongly correlated with V. anguillarum levels, where it comprised a significantly higher percentage of the microbiome in Oil/Pathogen challenged fish and was nearly non-existent in the No Oil/Pathogen challenged fish bacterial community. Elevated V. anguillarum levels were a direct result of oil exposure-induced immunosuppression. Oil-exposure reduced expression of immunoglobulin M, the major systemic fish antibody, and resulted in an overall downregulation in transcriptome response, particularly in genes related to immune function, response to stimulus and hemostasis. Ultimately, sediment-borne oil exposure impairs immune function, leading to increased incidences of bacterial infections. This type of sediment-borne exposure may result in long-term marine ecosystem effects, as oil-bound sediment in the northern Gulf of Mexico will likely remain a contamination source for years to come.

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“…Moreover, the 30% higher [TPH] measured in the oil + dispersant exposure tanks compared to the oil-alone tanks, indicated that dispersant increased the potential bioavailability of the oil components (Ramachandran et al, 2004). (Hellou andWarren, 1997 andVaranasi, 1989;Dussauze et al, 2015). Nevertheless, these concentrations are very similar to those measured in the white muscles of the rockfish (Sebastes schlegeli) 5 days following the Hebei Spirit oil spill (284 ng g -1 dry weight; Jung et al, 2011) and are in line with a previous experiment that investigated similar oil exposure conditions (148 ± 46 ng g -1 dry weight; Danion et al, 2011).…”

Section: Exposure Conditions and White Muscle [21 Pah]mentioning

confidence: 99%

Assessing chronic fish health: An application to a case of an acute exposure to chemically treated crude oil

et al. 2016

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Human alteration of marine ecosystems is substantial and growing. Yet, no adequate methodology exists that provides reliable predictions of how environmental degradation will affect these ecosystems at a relevant level of biological organization. The primary objective of this study was to develop a methodology to evaluate a fish's capacity to face a well-established environmental challenge, an exposure to chemically dispersed oil, and characterize the long-term consequences. Therefore, we applied high-throughput, non-lethal challenge tests to assess hypoxia tolerance, temperature susceptibility and maximal swimming speed as proxies for a fish's functional integrity. These whole animal challenge tests were implemented before (1 month) and after (1 month) juvenile European sea bass (Dicentrarchus labrax) had been acutely exposed (48h) to a mixture containing 0.08gL(-1) of weathered Arabian light crude oil plus 4% dispersant (Corexit© EC9500A), a realistic exposure concentration during an oil spill. In addition, experimental populations were then transferred into semi-natural tidal mesocosm ponds and correlates of Darwinian fitness (growth and survival) were monitored over a period of 4 months. Our results revealed that fish acutely exposed to chemically dispersed oil remained impaired in terms of their hypoxia tolerance and swimming performance, but not in temperature susceptibility for 1 month post-exposure. Nevertheless, these functional impairments had no subsequent ecological consequences under mildly selective environmental conditions since growth and survival were not impacted during the mesocosm pond study. Furthermore, the earlier effects on fish performance were presumably temporary because re-testing the fish 10 months post-exposure revealed no significant residual effects on hypoxia tolerance, temperature susceptibility and maximal swimming speed. We propose that the functional proxies and correlates of Darwinian fitness used here provide a useful assessment tool for fish health in the marine environment.

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Growth and immune system performance to assess the effect of dispersed oil on juvenile sea bass (Dicentrarchus labrax)

Cited by 19 publications

References 50 publications

An Experimental System to Assess Potential Biological Impact of Operational Response During an Oil Spill

An Experimental System to Assess Potential Biological Impact of Operational Response During an Oil Spill

Crude oil impairs immune function and increases susceptibility to pathogenic bacteria in southern flounder

Assessing chronic fish health: An application to a case of an acute exposure to chemically treated crude oil

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