Growth and hydrogen production of outdoor cultures of Synechocystis PCC 6803

Touloupakis, Eleftherios; Benavides, Ana Margarita Silva; Cicchi, Bernardo; Torzillo, Giuseppe

doi:10.1016/j.algal.2016.06.010

Cited by 22 publications

(10 citation statements)

References 48 publications

(53 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cyanobacteria are grown from cell density 0.1 to 5 g DCW /L to provide inoculum for the other reactors. Based on the available literature for outdoor cultivation in the different reactor formats (de Vree et al., ; Quinn et al., ; Touloupakis, Benavides, Cicchi, & Torzillo, ), we assume that growth in this phase is hybrid exponential‐linear and takes 5 days, regardless of reactor type.…”

Section: Methodsmentioning

confidence: 99%

“…For the G3 reactor, large‐scale, long‐term cultivations of microalgae in Fort Collins, Colorado (40° N) were used as a reference (maximum biomass productivity 0.37 g CDW /L/day; Quinn et al., ). For the TPBR, outdoor cultivations of cyanobacteria in 50 L reactors in Siesto Fiorentino, Italy (43° N) were used as a reference (biomass productivity 0.5 g CDW L –1 day –1 ; Touloupakis et al., ). For the FPBR, outdoor pilot‐scale microalgae cultivations in the Netherlands (51° N) were used as reference (biomass productivity 1.2 g CDW L –1 day –1 ; de Vree et al., ).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Environmental impacts and limitations of third‐generation biobutanol: Life cycle assessment of n‐butanol produced by genetically engineered cyanobacteria

Nilsson

Shabestary

Brandão

et al. 2019

J of Industrial Ecology

View full text Add to dashboard Cite

Photosynthetic cyanobacteria have attracted interest as production organisms for third‐generation biofuels, where sunlight and CO2 are used by microbes directly to synthesize fuel molecules. A particularly suitable biofuel is n‐butanol, and there have been several laboratory reports of genetically engineered photosynthetic cyanobacteria capable of synthesizing and secreting n‐butanol. This work evaluates the environmental impacts and cumulative energy demand (CED) of cyanobacteria‐produced n‐butanol through a cradle‐to‐grave consequential life cycle assessment (LCA). A hypothetical production plant in northern Sweden (area 1 ha, producing 5–85 m3 n‐butanol per year) was considered, and a range of cultivation formats and cellular productivity scenarios assessed. Depending on the scenario, greenhouse gas emissions (GHGe) ranged from 16.9 to 58.6 gCO2eq/MJBuOH and the CED from 3.8 to 13 MJ/MJBuOH. Only with the assumption of a nearby paper mill to supply waste sources for heat and CO2 was the sustainability requirement of at least 60% GHGe savings compared to fossil fuels reached, though placement in northern Sweden reduced energy needed for reactor cooling. A high CED in all scenarios shows that significant metabolic engineering is necessary, such as a carbon partitioning of >90% to n‐butanol, as well as improved light utilization, to begin to displace fossil fuels or even first‐ and second‐generation bioethanol.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Environmental impacts and limitations of third‐generation biobutanol: Life cycle assessment of n‐butanol produced by genetically engineered cyanobacteria

Nilsson

Shabestary

Brandão

et al. 2019

J of Industrial Ecology

View full text Add to dashboard Cite

show abstract

“…The start of Experiment #1 was at 14:00, the first measurement was at 20:00 (0 point). The start of Experiment #2 was at 17:00 (0 point), the first measurement was at 13:00 the next day (point 20 h) showed that photoinhibition strongly affected maximum quantum yield of PSII at low biomass concentration [17].…”

Section: The Hydrogen Production In Experiments #1mentioning

confidence: 99%

“…Recently, Synechocystis sp. strain PCC 6803 was cultivated outdoors in 50-L tubular PhBR, with maximum H 2 production rate of 0.05 mL L −1 h −1 under dark anaerobic conditions [17].…”

Section: Introductionmentioning

confidence: 99%

Plastic bags as simple photobioreactors for cyanobacterial hydrogen production outdoors in Moscow region

Shastik

Romanova

Laurinavichene

et al. 2019

Int J Energy Environ Eng

View full text Add to dashboard Cite

The application of transparent plastic bags with a gas barrier layer as inexpensive photobioreactors for H 2 production by the cyanobacterium Anabaena sp. PCC 7120 ∆Hup mutant cells was explored outdoors in Moscow region for the first time. Two experiments were performed in July and August, the average daily temperature being 21.7 and 20.6 °C, the average daily light intensity being 290 and 340 µmol photon m −2 s −1 in experiment #1 and #2, respectively. The maximal H 2 production rate was 20.6 mL day −1 L −1 of culture, with accumulation of 33.2 mL L −1 during 5 days and a final H 2 content of 1.1% (v/v). Molecular nitrogen added to the Ar gas at 3.3% significantly affected neither the rate nor the duration of H 2 production. Low morning temperatures as well as high daytime light intensities (especially at low cell concentrations) seemed to reduce the H 2 production rate. The activities obtained were lower as compared to the previously reported data. It could be attributable to suboptimal weather conditions and simple device arrangement. However, results prove that H 2 production by cyanobacteria is still feasible outdoors in plastic bags, the cheapest photobioreactors.

show abstract

“…Although this method can be easily implemented on a laboratory scale, it is not easy to scale if industrial production volumes are considered. Some attempts to carry out this process in tubular reactors have been described but always at working volumes lower than 100 L [80]. To improve the efficiency of this process, alternative approaches have been proposed, such as photobioreactors designed to increase cellular efficiency, integrate the production of microalgae with wastewater treatment, and even genetic engineering.…”

Section: Hydrogenmentioning

confidence: 99%

Microalgae biorefineries: applications and emerging technologies

Giraldo

Romo-Buchelly

Arbeláez-Pérez

et al. 2018

DYNA

View full text Add to dashboard Cite

Microalgae transform CO2 into a broad portfolio of biomolecules, and thus should be considered as a valuable biotechnological platform. Despite several research programs and global efforts to establish a sustainable microalgae-based industry, most applications have not transcended academic boundaries. This limitation raises from the high transformation costs when target products are biofuels or fertilizers. Microalgae biorefinery emerges as an alternative to improve economic competitiveness, as Under such a model scope, the process inputs come from industrial waste, while biomass exploitation prioritizes the highest value molecules followed by extraction of less valuable compounds. This review describes a wide range of microalgae exploitation schemes focused on new uses of its constituents, while discussing emerging technologies designed to improve production and efficiencies as well.Keywords: microalgae; biorefinery; biofuels; fertilizers; active biomolecules. Biorefinerías de microalgas: aplicaciones actuales y nuevas tecnologías en desarrolloResumen Las microalgas transforman el CO2 en un amplio portafolio de biomoléculas, por lo cual, son consideradas una valiosa plataforma biotecnológica. A pesar de múltiples programas de investigación y esfuerzos globales para establecer una industria sostenible basada en microalgas, la mayoría de las aplicaciones potenciales no han trascendido las fronteras académicas. Esta limitación se debe a los altos costos en la transformación del producto principalmente cuando se obtiene compuestos económicos como biocombustibles y fertilizantes. La biorefinería de microalgas surge como alternativa para incrementar la competitividad económica. En este modelo, los insumos del proceso provienen de residuos industriales, mientras que la explotación de la biomasa inicia con las moléculas de alto valor y finaliza con los compuestos menos valiosos. En esta revisión se describe un amplio abanico de esquemas de explotación de microalgas enfocado en nuevos usos de sus constituyentes. Además, se exploran las tecnologías emergentes destinadas a aprovechar esta biomasa de una manera más versátil y eficiente.

show abstract

Growth and hydrogen production of outdoor cultures of Synechocystis PCC 6803

Cited by 22 publications

References 48 publications

Environmental impacts and limitations of third‐generation biobutanol: Life cycle assessment of n‐butanol produced by genetically engineered cyanobacteria

Environmental impacts and limitations of third‐generation biobutanol: Life cycle assessment of n‐butanol produced by genetically engineered cyanobacteria

Plastic bags as simple photobioreactors for cyanobacterial hydrogen production outdoors in Moscow region

Microalgae biorefineries: applications and emerging technologies

Contact Info

Product

Resources

About