Growth and gaseous emissions of pure and mixed small intestinal bacterial cultures: Effects of bile and vancomycin

Hakalehto, Elias; Hell, Markus; Bernhofer, Christa; Heitto, Anneli; Pesola, Jouni; Humppi, Tarmo; Paakkanen, Heikki

doi:10.1016/j.pathophys.2009.07.003

Cited by 12 publications

(7 citation statements)

References 20 publications

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“…It has also been demonstrated that these deductions could be statistically verified by studying e.g. endoscopic samples, which in turn could give an idea of the microflora composition and interactions in different areas of the gastrointestinal tract [15]. In the same study, impacts of vancomycin and bile substances on the pure and mixed bacterial cultures were also investigated in the PMEU.…”

Section: Discussionmentioning

confidence: 94%

Enterobacterial Microflora in Infancy—A Case Study with Enhanced Enrichment

Pesola

Hakalehto

2011

Indian J Pediatr

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The enrichment step turned out to essentially improve the characterization and monitoring of the intestinal enterobacterial microbiota of infants. Compared to plate culture the amount of isolates was 2.6-fold by the PMEU enrichment. This study gives an idea on the development and succession of microbial communities in the gastrointestinal tract and on the variation of the strains due to the intestinal environmental factors.

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Section: Discussionmentioning

confidence: 94%

Enterobacterial Microflora in Infancy—A Case Study with Enhanced Enrichment

Pesola

Hakalehto

2011

Indian J Pediatr

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“…The BIB is an important constituent for our health and general well-being. It is based on the balance between different coliforms in the duodenum as the cornerstones [21,33]. There the absorption of food components usually begins, after the food is introduced into the duodenum from the acidic stomach.…”

Section: Bacteriological Intestinal Balance (Bib)mentioning

confidence: 99%

Current Megatrends in Food Production Related to Microbes

Hakalehto¹

2020

J Food Chem Nanotechnol

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The five major global phenomena or trends both in our thinking and in our societies that have strong links to the microbial kingdom: A. Human population growth continuously increases the demand for hygienically safe and adequate supplies to prevent qualitative and quantitative malnutrition. • Biorefineries and biotechnological techniques could be established on the basis of microbial bioengineering and understanding of natural microbial populations: this includes the reuse of food residues as well as the conversion of industrial and agricultural side streams into food sources. B. Requirements for sustainable and climate-friendly methods have also increased considerably. • Food ingredients can be produced from the above-mentioned additional resources which could be found within the existing processes and processes developed from them. C. Common understanding of the vital role of soil microbes in the food production has emerged globally. • Soil microbiota needs to be protected, and its role in the balances of carbon, nitrogen and other nutrients and elements need to be profoundly investigated. D. Relationship between our nutrition, health and microbiome have become more and more evident to the consumers. • Human microbiome has emerged as the core factor and contributor to our general well-being and health-the balance of the microbiome strongly influences the nutritional effects of our food, which have to be nurtured. E. Development of novel microbiological research and production methods. • Understanding the role of individual strains as parts of the community. While the global developments increase the movements of people and goods, an consequently, the rise of emerging epidemics and health problems, it is possible to resist those developments by implementing the potentials of microbial strains for producing food and chemical goods, as well as for cleaning up the environment.

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“…Different bacteria produce different volatiles and combined as mixed cultures they may produce different volatiles compared to pure state (Hakalehto, 2010;Hakalehto et al, 2010;Hakalehto, Jääskeläinen, Humppi, & Heitto, 2012). Therefore, it is fascinating to simulate different real life conditions in the PMEU environment, and to study how different bacteria do behave together in nature or in our body (e.g.…”

Section: Basics Of the Pmeu Technologymentioning

confidence: 99%

“…The tendency of mixed intestinal microflora to build up small scale ecosystems has enormous beneficial effects on the host body system, for example preventing pathogens to colonize gastrointestinal system, or to facilitate nutrients absorption by the host. With the PMEU approach, the effects of pH, bile substances, gases, antibiotics and defensins on various bacterial populations, have been studied (Hakalehto, Humppi, & Paakkanen, 2008;Hakalehto et al, 2010;Hakalehto, 2011a, b). Various isolates from the endoscopic and colonoscopic specimens were also investigated by PMEU methods Hell et al, 2010).…”

Section: Simulations Of the Alimentary Microbiomementioning

confidence: 99%

Minute Microbial Levels Detection in Water Samples by Portable Microbe Enrichment Unit Technology

Hakalehto¹,

Heitto²

2012

ENRR

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Water purity requires continuous monitoring. PMEU technology (Portable Microbe Enrichment Unit) provides the means for starting the enrichment and analysis immediately after the sampling. The entire procedure can be automated and microbe levels monitored in real-time from the enrichment broth by using IR, optical, UV or volatiles screening. For example, single cells of Escherichia coli have been verified from water samples in less than 10 hours in an official validation project. Also such pathogens as Salmonella, Campylobacter and Yersinia have been detected rapidly from natural, purified and irrigation waters. The PMEU technology has potential in the continuous monitoring within water departments and distribution systems, as well as in environmental studies.Keywords: rapid microbe detection, water hygiene, indicator bacteria, ColilertTM, PMEU, environmental monitoring, pathogens, water distribution BackgroundIn the present manuscript experimental approach and work carried out with the Portable Microbe Enrichment Unit (PMEU) is presented. This device is an ideal tool to investigate micro-organisms life at microscopic and molecular scale. Micro-organisms such as bacteria have a large influence on our lives with impact on economy, various industries, healthcare, agriculture and forestry, as well as in water management. In Finland our team published booklet entitled "Vesien Mikrobiaapinen" (in English: "A Microbiological ABC book of the waters") with the purpose to provide microbiological information to decision makers. As scientists we continuously deal with the public and their representatives: the political decision makers. In order to get funding, we ought to make them understand the importance of environmental protection and conservation of water sources, from the microbiological point of view.One area where micro-organisms have a large influence is the threat and deleterious impact on human and animal health caused by pathogenic micro-organisms. They are potentially distributed via waterways and often disseminated at small concentrations which still pose health hazards. In order to detect these minute concentrations, new tools are needed, also to simulate their biochemical activities. Their detection is not either a single or a simple event but a whole diagnostic process (Figure 1). Focusing on the often neglected step of specimen collection is of crucial importance. Sampling should be carried out considering all the physicochemical parameters, selection of sampling site etc. Usually it is required to transport and store the sample with caution and care. This is the step when most of the microbes present in the sample are often destroyed. They simply cannot survive or do not remain microbiologically viable. In order to avoid these problems, the Portable Microbe Enrichment Unit was developed. Time is saved in the PMEU processing of the samples, since the verification steps could follow sooner compared to traditional microbiology. The basic simple idea is to start the enrichment cultivation right subseq...

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Growth and gaseous emissions of pure and mixed small intestinal bacterial cultures: Effects of bile and vancomycin

Cited by 12 publications

References 20 publications

Enterobacterial Microflora in Infancy—A Case Study with Enhanced Enrichment

Enterobacterial Microflora in Infancy—A Case Study with Enhanced Enrichment

Current Megatrends in Food Production Related to Microbes

Minute Microbial Levels Detection in Water Samples by Portable Microbe Enrichment Unit Technology

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