Growth and Function of the Pancreatic β cell in vitro

Nielsen, Jens Høiriis

doi:10.1530/acta.0.109s007

Cited by 5 publications

(9 citation statements)

References 78 publications

(107 reference statements)

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“…Long-term culture of isolated pancreatic islets from rodents has been thoroughly examined during the last 20 yr. A low serum concentration in the medium is preferable (13). In the present paper, we have demonstrated a significantly higher daily insulin and somatostatin secretion in serum-free medium.…”

Section: R E S U L T S a N D Discussionsupporting

confidence: 58%

“…Regular insulin secretion was measured to control the functional level of pancreatic islets in long-term culture (13). Thus, insulin was measured after Days 3, 5, and 7 of the experimental culture period.…”

Section: R E S U L T S a N D Discussionmentioning

confidence: 99%

“…Marked differences in serum requirements for culturing of mouse, rat, and human islets have also been described (1,2,4,13). Nielsen (13) reported human serum (HS) to be superior to sera from other species as welt as homologous serum. The factor(s) responsible for the beneficial effects of serum on islet culture has not yet been identified.…”

Section: Introductionmentioning

confidence: 99%

“…The factor(s) responsible for the beneficial effects of serum on islet culture has not yet been identified. A protein of 60 to 100 kDa, which is abundantly found in serum from diabetic patients, has been proposed (13) as being the essential stimulating serum factor.…”

Section: Introductionmentioning

confidence: 99%

“…Serum also has several disadvantages, e.g., variable protein content, presence of peptide degrading factors, and general cytotoxic effects (10,13). Albumin and transferrin are frequently used as serum replacements to avoid these problems.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Effect of a new synthetic serum replacement on insulin and somatostatin secretion from isolated rat pancreatic islets in long-term culture

Vonen

Bertheussen

Giæver

et al. 1992

Journal of Tissue Culture Methods

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SUMMARY: Serum contains insulin degrading components. We have evaluated the insulin and somatostatin secretion from isolated rat pancreatic islets during a 2-wk culture period using three different serum-containing media, and one serum-free medium with a synthetic serum replacement. Islets incubated in serum-free medium elicited significantly higher daily insulin and somatostatin secretions than islets incubated in the serum-containing media. After a 2-wk culture period, islets from the serum-free medium secreted significantly more insulin and somatostatin than islets cultured in other media when stimulated with 25 retool/liter glucose together with 15 mmol/liter theophylline. We conclude that the serum-free medium is superior for long-term culture of rat pancreatic islets.

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Section: R E S U L T S a N D Discussionsupporting

confidence: 58%

Section: R E S U L T S a N D Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effect of a new synthetic serum replacement on insulin and somatostatin secretion from isolated rat pancreatic islets in long-term culture

Vonen

Bertheussen

Giæver

et al. 1992

Journal of Tissue Culture Methods

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Models of pancreatic regeneration in diabetes

Risbud¹,

Bhonde²

2002

Diabetes Research and Clinical Practice

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Role of increased insulin demand in the adaptation of the endocrine pancreas to pregnancy

Nieuwenhuizen

Schuiling

Moes

et al. 1997

Acta Physiologica Scandinavica

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During gestation the demand for insulin increases due to a decrease in insulin sensitivity of the maternal tissues. Simultaneously, pancreatic islet-cell proliferation, as well as insulin production and secretion increase. Both phenomena appear to be caused by the actions of pregnancy hormones. We studied the relationship between the two phenomena by investigating whether the supply of exogenous insulin affects the secretion of pregnancy hormones and islet function during gestation. For that purpose rats were treated with high doses of insulin (4.8 IU day-1 by sub-cutaneous osmotic mini pumps) so that the endogenous demand for insulin was fully satisfied from day 8-14 of gestation. Euglycaemia (5.0 mM) was maintained by intra venous infusion of glucose. The treatment suppressed insulin synthesis, as measured by in situ hybridization, in both pregnant and cyclic rats. In addition, in pregnant rats the increments in insulin secretion and in islet-cell proliferation were partly prevented. Furthermore, the data also suggest that in pregnant rats the treatment partly prevented the decrease in insulin sensitivity. Finally, the treatment did not affect the plasma concentrations of progesterone, prolactin and placental lactogen, but prevented the rise in growth hormone concentrations in pregnant rats. The present data suggest that, next to direct effects of pregnancy hormones and growth hormone on the pancreatic islets, a decreased insulin sensitivity in the maternal tissues, induced by actions of the same hormones, is involved in the regulation of islet function during gestation.

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Growth and Function of the Pancreatic β cell in vitro

Cited by 5 publications

References 78 publications

Effect of a new synthetic serum replacement on insulin and somatostatin secretion from isolated rat pancreatic islets in long-term culture

Effect of a new synthetic serum replacement on insulin and somatostatin secretion from isolated rat pancreatic islets in long-term culture

Models of pancreatic regeneration in diabetes

Role of increased insulin demand in the adaptation of the endocrine pancreas to pregnancy

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