Growth and Formation of the Tooth Germ in a Rat Model of Fetal Alcohol Syndrome

Imai, Rie; Miake, Yasuyo; Yanagisawa, Takaaki; Yakushiji, Masashi

doi:10.2485/jhtb.16.61

Cited by 2 publications

(4 citation statements)

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“…In the present study, however, such histological alterations were not observed in the tooth germs in the 25%-ethanolexposed rats. These findings agree with those of other investigators 21) , who found that alcohol intake during pregnancy had no significant influence on cell morphogenesis or differentiation, despite a few days delay in tooth germ development in the ethanolintake rats. In evaluating the effect of ethanol on tooth development in an animal model, it is important to take into account the species and strain of animal used, as their ability to metabolize alcohol will affect ethanol concentration, administration route, duration of administration and period of exposure to be employed.…”

Section: Discussionsupporting

confidence: 93%

“…Taken together with those of earlier studies 20,21) , the results of this study suggest that ingestion of ethanol during pregnancy alters fetal rat growth in a variety of ways, including litter size and body weight, although isocaloric pair-fed rats were not employed as controls. In addition, many reports have shown that ethanol suppresses development of other parts of the body, including the brain [22][23][24] , liver 25) , cranial bone and mandible 26,27) in an animal FAS model.…”

Section: Discussionsupporting

confidence: 69%

“…In addition, many reports have shown that ethanol suppresses development of other parts of the body, including the brain [22][23][24] , liver 25) , cranial bone and mandible 26,27) in an animal FAS model. However, few studies 20,21,28) have investigated the influence of ethanol on tooth development. The aim of the present study was, therefore, to evaluate whether alcohol ingestion in pregnant rats altered tooth development and cellular expression of growth factors.…”

Section: Discussionmentioning

confidence: 99%

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Morphometric and immunohistochemical investigation of tooth development in rats prenatally exposed to ethanol

Oda

Sawada

Yanagisawa

et al. 2009

Pediatric Dental Journal

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The purpose of this study was to clarify the effect of ethanol on rat molar tooth development. Pregnant rats were fed 25%-ethanol solutions or ethanol-free water up to delivery, and tooth germs, as well as fully developed teeth, obtained from the offspring (ethanol-exposed rats or control rats) were subjected to morphometric analysis and immunohistochemistry. No significant differences were observed in terms of tooth size between the ethanol-exposed and control rats. Moreover, no abnormalities in cusp number, form or dental surface structure was noted in the ethanol-exposed rats. In the ethanol-exposed rats, the tooth development showed a slight retardation in comparison to the controls. Morphological alteration in tooth germs was not observed in the experimental rats. Both bone morphogenetic protein-4 and basic fibroblast growth factor-2 were immunolocalized in the cells composing the tooth germs in the ethanol-exposed rats. No differences, however, were found in immunostaining intensity between the ethanol-exposed and control rats. Taken together, the results indicate that the effect of ethanol on tooth development was weaker than expected in the rat FAS model. which looked at anthropometric measurements and oral health status in FAS children found no significant difference in prevalence of enamel opacities between FAS patients and controls 5). Thus, although there seems to be some relationship between intake of alcohol during pregnancy and abnormal tooth development in children, the precise nature of this relationship remains to be clarified. Tooth development results from a process of mutual cellular proliferation and differentiation in epithelial and mesenchymal cells in the tooth germ during the fetal stage 8,9). A variety of growth factors act as inductive signals in embryonic tissue interaction 8-10). Among them, bone morphogenetic proteins (BMPs) 11-14) , fibroblast growth factors (FGFs) 15-17) and epidermal growth factor (EGF) 18,19) are believed to play a particularly important role in the process of tooth development. Therefore, in order to obtain a better understanding of how alcohol intake affects tooth development, it is necessary to study the effect

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 69%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Morphometric and immunohistochemical investigation of tooth development in rats prenatally exposed to ethanol

Oda

Sawada

Yanagisawa

et al. 2009

Pediatric Dental Journal

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show abstract

“…These data corroborate the histological report observed in the 20-days-old group. They are also in agreement with the histological findings of Imai et al (2007) that in 20-days-old rats, tooth morphogenesis is almost complete and tooth eruption occurs.…”

Section: Discussionsupporting

confidence: 91%