Growth and fine structure of monolayers derived from adult rat adenohypophyseal cell suspensions

Rappay, G; Gyévai, A; Kondics, L; Stark, E.

doi:10.1007/bf02615910

Cited by 32 publications

(4 citation statements)

References 22 publications

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“…Cell culture Cells prepared from the anterior pituitary glands of about 20 rats according to the method of Rappay, Gyévai, Kondics & Stark (1973) and Rappay, Nagy, Makara, Bácsy, Fazekas, Kárteszi & Kurcz (1979), were plated at a density of 105/well in 96 well microplates (Corning). The culture medium consisted of 100pi DMEM, fortified with 2-5% (v/v) fetal calf serum, 10% (v/v) horse serum and 01% (v/v) non-essential amino acid mixture (Flow Laboratories).…”

Section: Electrical Stimulationmentioning

confidence: 99%

Growth Hormone Releasing Activity Persists in the Deafferented Medial-Basal Hypothalamus of the Rat

Antoni¹,

Makara²,

Rappay³

1981

Journal of Endocrinology

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The possible role of the neural connections of the medial-basal hypothalamus (MBH) in the maintenance of GH releasing activity of the pituitary stalk median eminence (SME) was investigated. Male rats, subjected to sham-operation and to complete and anterolateral cuts around the MBH were used 7-8 days after surgery. Electrical stimulation of neural structures within the MBH caused an increase of plasma GH in pentobarbitone- as well as in urethane-anaesthetized animals. In sham-operated rats the rise of plasma GH levels was apparent only after completion of 10 min of electrical stimulation, while in animals with complete or anterolateral cuts an increase was already evident during electrical stimulation. The results suggest that depolarization of somatostatin secreting fibres in the median eminence may be responsible for the delay in the rise of GH levels in sham-operated rats, while the increment can be attributed to a GH releasing principle in the hypothalamus. Acidic extracts of the SME of rats with complete or anterolateral cuts stimulated the release of GH by primary cultures of rat anterior pituitary cells. Microinjection of 0.05 SME equivalents of SME extract into the anterior pituitary gland of urethane-anaesthetized rats produced a rise in plasma GH levels within 3 min of injection. These data favour the existence of a GH releasing factor, and suggest that the ventromedial and arcuate hypothalamic nuclei are major sites of production of this releasing hormone.

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Section: Electrical Stimulationmentioning

confidence: 99%

Growth Hormone Releasing Activity Persists in the Deafferented Medial-Basal Hypothalamus of the Rat

Antoni¹,

Makara²,

Rappay³

1981

Journal of Endocrinology

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“…This concept involves complicated regulatory mechan isms, some details of which are already known, others are being unveiled. Cultures derived from dispersed cells of the anterior lobe of the rat hypophysis have been proved useful for the study of some of these mechanisms [Vale et al, 1972;Steinberger et al, 1973;Tixier-Vidal et al, 1973;Takebeet al, 1975;Langet al, 1975;Vale and Rivier, 1977;Wilfinger et al, 1979], While papers dealing with the physiology of cultured pituitary cells are numerous, there are only a few reporting on their fine struc ture [Rappay et al, 1973;Tixier-Vidal, 1975;Bâcsy et al, 1976]. No detailed analysis of the surface characteristics of the adult rat pituitary anterior lobe cells in cul ture has been published.…”

Section: Introductionmentioning

confidence: 99%

Surface Topography of Cultured Anterior Pituitary Cells of the Rat

Fazekas¹,

Bácsy²,

Rappay³

1982

Cells Tissues Organs

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Anterior pituitary cells of the adult rat were studied by scanning electron microscopy after 3, 6 and 8 days in culture. Epithelial (parenchymal) cells and fibroblasts could be differentiated by their characteristic cell surface structures and by their associations with each other. The surface of solitary and grouped epithelial cells is uneven and rich in smaller or larger blebs, wrinkles and microvilli. A few solitary cilia were found on the surface of some cells. The individual cells forming epithelial cell groups adhere to each other with numerous, parallel, slender extensions. The fibroblasts found in monolayers have extensions with various lengths contacting other cells or the inert substratum. Microvilli arise in various frequency from their smooth surface. Transmission electron microscopy confirmed the presence of the surface structures detected in the scanning electron microscope.

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“…In Vitro Experiments. Twenty, female, 160-200-g SPF rats (LATI, Godoll6, Hungary) were used for preparing the pituitary tissue cultures according to the method of Rappay et al (22). The animals were anesthetized (Sodium Pentobarbital, 40 mgkg), and the pituitaries were excised and placed into M199 tissue culture medium (Sigma Chemical Co., St. Louis, MO).…”

Section: Methodsmentioning

confidence: 99%

Ethanol's Effect on Rat Pituitary Adrenal Axis is Prevented by Purine Metabolic Pathway Inhibitors

Szabó

Bruckner

Medveczky

et al. 1999

Experimental Biology and Medicine

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This study investigated the in vitro dose effects of ethanol (EtOH), adenosine (ADO), and urate (URA) on the basal and CRF stimulated ACTH production of pituitary tissue culture (PTC). Furthermore, the effects of low (LE = 0.5 g k g bodyweight) and high (HE = 2.5 g k g bodyweight) concentrations of EtOH were tested in rats on plasma ACTH and corticosterone concentration (PCC), with or without the following ADO metabolic pathway inhibitors: 6-mercaptopurine (6MP) (salvage pathway) and purpurogallin (PPG) (xanthine dehydrogenase).M) stimulated ACTH secretion in PTC; in fact doses up to 20 mM tended to be inhibitory. ADO significantly increased only basal ACTH secretion whereas URA increased both basal and CRF-stimulated ACTH secretion.Pretreatment with PPG or 6MP + PPG significantly increased both in vivo ACTH and PCC over control values in rats. HE versus LE significantly increased ACTH and PCC in the control (H,O) and 6MP pretreated groups whereas in the PPG pretreated animals, only ACTH was increased significantly by HE. However, combined pretreatment with 6MP + PPG prevented the effect of HE on ACTH and PCC. The current experiment suggests that purine metabolism is involved in ethanol's effect on the hypothalamic-pituitary-adrenal axis.

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Growth and fine structure of monolayers derived from adult rat adenohypophyseal cell suspensions

Cited by 32 publications

References 22 publications

Growth Hormone Releasing Activity Persists in the Deafferented Medial-Basal Hypothalamus of the Rat

Growth Hormone Releasing Activity Persists in the Deafferented Medial-Basal Hypothalamus of the Rat

Surface Topography of Cultured Anterior Pituitary Cells of the Rat

Ethanol's Effect on Rat Pituitary Adrenal Axis is Prevented by Purine Metabolic Pathway Inhibitors

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