Growth and fatty acid composition of juvenile<i>Cerastoderma edule</i>(L.) fed live microalgae diets with different fatty acid profiles

Batista, Isabel Reis; Kamermans, P.; Verdegem, Marc; Smaal, A.C.

doi:10.1111/anu.12059

Cited by 9 publications

(8 citation statements)

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“…Early studies recognised lipids to be the main energy stores for bivalves up to 6 months post-settlement [25,26]. Since then, evaluating the lipid composition of diets and juveniles gained a central importance on bivalve nutrition studies with examples available for clams [27][28][29][30][31][32][33], scallops [34][35][36], oysters [37][38][39][40][41][42] and mussels [19,[43][44][45][46]. Other than protein and carbohydrate composition, the nutritional properties of shellfish diets strongly depend on the essential PUFA (arachidonic acid-20:4n-6, AA; eicosapentaenoic acid-20:5n-3, EPA; docosahexaenoic acid-22:6n-3, DHA) content [47].…”

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confidence: 99%

Lipidomics analysis of juveniles’ blue mussels (Mytilus edulis L. 1758), a key economic and ecological species

et al. 2020

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Blue mussels (Mytilus edulis L. 1758) are important components of coastal ecosystems and in the economy of rural and coastal areas. The understanding of their physiological processes at key life stages is important both within food production systems and in the management of wild populations. Lipids are crucial molecules for bivalve growth, but their diversity and roles have not been fully characterised. In this study, traditional lipid profiling techniques, such as fatty acid (FA) and lipid class analysis, are combined to untargeted lipidomics to elucidate the lipid metabolism in newly settled spat fed on a range of diets. The evaluated diets included single strains treatments (Cylindrotheca fusiformis CCAP 1017/2 -CYL, Isochrysis galbana CCAP 927/1-ISO, Monodopsis subterranean CCAP 848/1 -MONO, Nannochloropsis oceanica CCAP 849/10-NANNO) and a commercial algae paste (SP). Spat growth was influenced by the diets, which, according to their efficacy were ranked as follows: ISO>NANNO/CYL>SP>MONO. A higher triacylglycerols (TG) content, ranging from 4.23±0.82 μg mg ashfree Dry weight (DW) -1 at the beginning of the trial (T0) to 51 OPEN ACCESS Citation: Laudicella VA, Beveridge C, Carboni S, Franco SC, Doherty MK, Long N, et al. (2020) Lipidomics analysis of juveniles' blue mussels (Mytilus edulis L. 1758), a key economic and ecological species. PLoS ONE 15(2): e0223031.

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confidence: 99%

Lipidomics analysis of juveniles’ blue mussels (Mytilus edulis L. 1758), a key economic and ecological species

et al. 2020

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“…Results in this study indicated that a diet low in ARA content did not limit the growth and survival of juveniles when EPA and DHA content in the diet was balanced. In the common cockle, Cerastoderma edule , relatively higher ARA content was found in the tissues of juveniles fed microalgae diets with low ARA content, and ARA content did not limit juvenile growth (Reis Batista et al., 2014). Hurtado et al.…”

Section: Discussionmentioning

confidence: 99%

“…Due to the inability of bivalves to ‘de novo’ synthesise long‐chain essential fatty acids from shorter chain precursors, the requirement of essential fatty acids must be met through diet. Microalgae species rich in n ‐3 polyunsaturated fatty acids (PUFA) such as eicosapentaenoic acid (EPA; 20:5 n ‐3) and docosahexaenoic acid (DHA; 22:6 n ‐3) support better growth and survival at the early life stage (Liu et al., 2016; Reis Batista et al., 2014). The n ‐6 PUFAs linoleic acid (18:2 n ‐6) and arachidonic acid (ARA; 20:4 n ‐6) act as metabolic precursors of EPA and DHA synthesis in bivalves; therefore, the ratio of n ‐3/ n ‐6 also influences the quality of a bivalve diet (Soudant et al., 1999).…”

Section: Introductionmentioning

confidence: 99%

“…The n ‐6 PUFAs linoleic acid (18:2 n ‐6) and arachidonic acid (ARA; 20:4 n ‐6) act as metabolic precursors of EPA and DHA synthesis in bivalves; therefore, the ratio of n ‐3/ n ‐6 also influences the quality of a bivalve diet (Soudant et al., 1999). Significant research efforts have been made to evaluate the dietary influence of fatty acids profile in many bivalve species, including oysters (Ronquillo et al., 2012; Wikfors et al., 1996), mussels (Nevejan et al., 2007), clams (Albentosa et al., 1996; Fernández‐Reiriz et al., 2006; Reis Batista et al., 2014) and scallops (Milke et al., 2004). Insufficient dietary fatty acids coupled with a low‐temperature environment triggered higher mortality rates in Mercenaria mercenaria juveniles (Portilla, 2016; Portilla et al., 2015), which demands a better understanding of a suitable microalgae diet.…”

Section: Introductionmentioning

confidence: 99%

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Growth, survival and fatty acid profile of hard clam, Mercenaria mercenaria, juveniles fed live microalgae diets

Hassan

Perri

Parks³

et al. 2022

Aquaculture Fish & Fisheries

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Choice of microalgae diet affects production outcomes in bivalve hatcheries. This study aimed to identify an optimal microalgae diet to improve hard clam, Mercenaria mercenaria, juvenile production. Four microalgae species (Tisochrysis lutea, Pavlova lutheri, Chaetoceros gracilis and Cyclotella nana) in six dietary combinations were fed for 6 weeks; then, growth, survival and fatty acid profiles of juveniles were evaluated. The highest numerical growth and survival of juveniles were attained by feeding a tetra-species microalgae diet, but no statistical difference was found by feeding bior tri-species diets that consisted of a mix of flagellate and diatom microalgae. Significant differences in fatty acid profiles were found among clams fed different microalgae diets. A contrasting polyunsaturated fatty acid (PUFA) profile was found in juveniles fed flagellate-or diatom-only diets. Clams fed flagellate-only diets contained a higher percentage of docosahexaenoic acid (DHA) but a lower percentage of (n-6) PUFAs, whereas those fed diatom-only diets had a higher percentage of arachidonic acid (ARA) and eicosapentaenoic acid (EPA) but a lower percentage of DHA. In contrast, the percentage of EPA, DHA and ARA was neither very high nor very low in juveniles fed the tetra-species diet. The complementary nutritional profile of the tetra-species microalgae diet resulted in enhanced production and exhibited a more balanced fatty acid profile in clam tissue. The tetra-species microalgae diet should be a preferable choice for feeding hard clam juveniles, but the cost of producing multiple microalgae species for hatchery/nursery operations should be taken into consideration as bi-and trispecies diets provided statistically similar growth and survival. Results of this study can be used to improve productivity and profitability in M. mercenaria hatcheries.

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“…Early studies recognised lipids to be the main energy stores for bivalves up to 6 months post-settlement [25, 26]; Since then, evaluating the lipid composition of diets and juveniles gained a central importance on bivalve nutrition studies with examples available for clams [27–33], scallops [34–36], oysters [37–42] and mussels [19, 43–46]. Other than protein and carbohydrate composition, the nutritional properties of shellfish diets strongly depend on the essential PUFA (arachidonic acid – 20:4n-6, AA; eicosapentaenoic acid – 20:5n-3, EPA; docosahexaenoic acid – 22:6n-3, DHA) content [47].…”

Section: Introductionmentioning

confidence: 99%

Lipidomics analysis of juveniles’ blue mussels (Mytilus edulisL. 1758), a key economic and ecological species

Laudicella

Beveridge

Carboni

et al. 2019

Preprint

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Blue mussels (Mytilus edulis L.) are important components of coastal ecosystems functioning through benthopelagic coupling and ecosystem engineering. At the same time, mussel production is central in the economy of coastal areas. Therefore, understanding their nutritional, physiological and metabolic processes at key life stages is important for their management, both within food production systems and in wild populations.Lipids are crucial molecules for bivalve growth, but their diversity and roles have been considered from fatty acid (FA) perspective. In this paper, we applied lipidomics to bivalve nutrition. Lipidomics provides a holistic perspective on lipid patterns; by examining the lipidome, important physiological information can be acquired. Here, we use controlled laboratory experiments to elucidate the responses to changes in the diet of newly settled mussels juveniles, one of the most critical life stages. The diets considered in this study are single strains diet of Cylindrotheca fusiformis CCAP 1017/2 – CYL, Isochrysis galbana CCAP 927/1– ISO, Monodopsis subterranean CCAP 848/1 – MONO, Nannochloropsis oceanica CCAP 849/10– NANNO and a commercial algae paste –SP.The diets had a significant effect on spat GR and WI, and according to their efficacy resulted ranked as follows: ISO>NANNO/CYL>SP>MONO. Spat FA composition and neutral lipid content (principally triacylglycerols - TG), were influenced by the diets. Furthermore, untargeted lipidomics also showed shifts in several phospholipid species, with changes related to the essential PUFA available from the diet. TG content, neutral lipids and several TG and FA species were correlated (Spearman R2>0.8 FDR p<0.05) with spat WI, suggesting their possible application as markers of mussel juvenile condition. The availability of dietary essential PUFA deeply modified the spat lipidome both for neutral and for polar lipids. This change in the lipidome could have major impacts on their ecology and their production for food.

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Growth and fatty acid composition of juvenileCerastoderma edule(L.) fed live microalgae diets with different fatty acid profiles

Cited by 9 publications

References 30 publications

Lipidomics analysis of juveniles’ blue mussels (Mytilus edulis L. 1758), a key economic and ecological species

Lipidomics analysis of juveniles’ blue mussels (Mytilus edulis L. 1758), a key economic and ecological species

Growth, survival and fatty acid profile of hard clam, Mercenaria mercenaria, juveniles fed live microalgae diets

Lipidomics analysis of juveniles’ blue mussels (Mytilus edulisL. 1758), a key economic and ecological species

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