Growth and enzymatic responses of phytopathogenic fungi to glucose in culture media and soil

Abstract

The effect of inoculation of Aspergillus flavus, Fusarium verticillioides, and Penicillium sp. in Dystrophic Red Latosol (DRL) and Eutroferric Red Latosol (ERL) soils with or without glucose on the total carbohydrate content and the dehydrogenase and amylase activities was studied. The fungal growth and spore production in culture medium with and without glucose were also evaluated. A completely randomized design with factorial arrangement was used. The addition of glucose in the culture medium increased the growth rate of A. flavus and Penicillium sp. but not of F. verticillioides. The number of spores increased 1.2 for F. verticillioides and 8.2 times for A. flavus in the medium with glucose, but was reduced 3.5 times for Penicillium sp. The total carbohydrates contents reduced significantly according to first and second degree equations. The consumption of total carbohydrates by A. flavus and Penicillium sp. was higher than the control or soil inoculated with F. verticillioides. The addition of glucose to soils benefited the use of carbohydrates, probably due to the stimulation of fungal growth. Dehydrogenase activity increased between 1.5 to 1.8 times (p <0.05) in soils with glucose and inoculated with the fungi (except F. verticillioides), in relation to soil without glucose. Amylase activity increased 1.3 to 1.5 times due to the addition of glucose in the soil. Increased amylase activity was observed in the DRL soil with glucose and inoculated with A. flavus and Penicillium sp. when compared to control

Extracts of Amazonian Fungi With Larvicidal Activities Against Aedes aegypti

“…To avoid differences in nutrient availability, we used total glucose consumption in the culture medium as determinant of the cultivation time length. Fungi growth styles and physical, chemical and biological factors, among others, influence development time and the production of bioactive metabolites (Kavanagh, 2011;Costa and Nahas, 2012). Speciesspecific traits explain the difference in cultivation time and biological activities among the isolated fungi studied in our work.…”

Effect of Scots Pine Sawdust Amendment on Abundance and Diversity of Culturable Fungi in Soil

“…Cellulolysis provides carbohydrates (the breakdown products) shortly after sawdust incorporation into soil. Theoretically, the abundance of carbohydrates should have stimulated the growth and reproduction of microorganisms [16]. However, the communities described here only rarely behaved according to such a scheme.…”

Uji In Vitro Keefektifan Ekstrak Air Daun Dan Bunga Kembang Telang (Clitoria ternatea l.) terhadap Jamur Alternaria solani Penyebab Penyakit Bercak Coklat pada Tanaman Tomat

“…Stimulasi peningkatan jumlah konidia jamur dapat terjadi karena berbagai pengaruh, misalnya kurang tersedianya senyawa gula yang dibutuhkan untuk sporulasi seperti dilaporkan pada jamur Aspergillus flavus (Calvo et al, 2002) dan kurangnya nutrisi pada Penicillium sp. (Costa & Nahas, 2012).…”