Growth and Differentiation of Neural Cells in Defined Media

Bottenstein, Jane E.

doi:10.1007/978-1-4613-2473-7_1

Cited by 100 publications

(77 citation statements)

References 127 publications

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“…For this purpose, we developed a novel two-step protocol that induces MSCs toward the NTF-secreting astroglial lineage as opposed to the neuronal lineage. The induction protocol included growth factors known to induce neural differentiation in MSCs, such as epidermal growth factor, basic fibroblast growth factor, and PDGF [33,43], and N2 cocktail, which is reported to have increased survival of neural primary cultures and differentiation [44]. Cyclic AMP, an important intracellular secondary messenger, is also linked to this process [39,45].…”

Section: Discussionmentioning

confidence: 99%

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Migration of Neurotrophic Factors-Secreting Mesenchymal Stem Cells Toward a Quinolinic Acid Lesion as Viewed by Magnetic Resonance Imaging

et al. 2008

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Section: Discussionmentioning

confidence: 99%

“…In the imaging field, it is difficult to delineate implanted cells from host tissue without contrast agents or markers. In a recent study, optical imaging with luciferase expression has been used to prove the migration of neural stem cells in the QA model of HD [1,44].…”

Section: Discussionmentioning

confidence: 99%

Migration of Neurotrophic Factors-Secreting Mesenchymal Stem Cells Toward a Quinolinic Acid Lesion as Viewed by Magnetic Resonance Imaging

et al. 2008

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“…F3 antigenic sites become concentrated on neurites in cultures maintained in chemically defined medium. Cultures prepared from embryonic day-15 mouse forebrains were grown for 5 d in the medium formulated by Bottenstein (1985). Live cup tures were stained with anti-Dl.1 antibodies using FITC-conjugated anti-(rabbit IgG) (Fab)2 (B).…”

Section: Discussionmentioning

confidence: 99%

The mouse neuronal cell surface protein F3: a phosphatidylinositol-anchored member of the immunoglobulin superfamily related to chicken contactin.

Gennarini

Cibelli

Rougon

et al. 1989

The Journal of Cell Biology

259

166

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Abstract. Several members of the Ig superfamily are expressed on neural cells where they participate in surface interactions between cell bodies and processes. Their Ig domains are more closely related to each other than to Ig variable and constant domains and have been grouped into the C2 set. Here, we report the cloning and characterization of another member of this group, the mouse neuronal cell surface antigen F3. The F3 eDNA sequence contains an open reading frame that could encode a 1,020-amino acid protein consisting of a signal sequence, six Ig-like domains of the C2 type, a long premembrane region containing two segments that exhibit sequence similarity to fibronectin type III repeats and a moderately hydrophobic COOH-terminal sequence. The protein does not contain a typical transmembrane segment but appears to be attached to the membrane by a phosphatidylinositol anchor. Antibodies against the F3 protein recognize a prominent 135-kD protein in mouse brain. In fetal brain cultures, they stain the neuronal cell surface and, in cultures maintained in chemically defined medium, most prominently neurites and neurite bundles. The mouse f3 gene maps to band F of chromosome 15. The gene transcripts detected in the brain by F3 cDNA probes are developmentally regulated, the highest amounts being expressed between 1 and 2 wk after birth.The F3 nucleotide and deduced amino acid sequence show striking similarity to the recently published sequence of the chicken neuronal cell surface protein contactin. However, there are important differences between the two molecules. In contrast to F3, contactin has a transmembrane and a cytoplasmic domain. Whereas contactin is insoluble in nonionic detergent and is tightly associated with the cytoskeleton, about equal amounts of F3 distribute between buffer-soluble, nonionic detergent-soluble, and detergent-insoluble fractions. Among other neural cell surface proteins, F3 most resembles the neuronal cell adhesion protein L1, with 25 % amino acid identity between their extracellular domains. Based on its structural similarity with known cell adhesion proteins of nervous tissue and with L1 in particular, we propose that F3 mediates cell surface interactions during nervous system development.

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“…C ells were plated at a density of ϳ2000 cells/cm 2 on substrate-coated glass coverslips in M EM containing 10% horse serum. After 2 hr were allowed for cell attachment, the coverslips were transferred to dishes containing a confluent monolayer of glia in serumfree M EM with N2 supplements (Bottenstein and Sato, 1979;Bottenstein, 1985), 0.1% ovalbumin, and 0.1 mM pyruvate.…”

Section: Cell Culturementioning

confidence: 99%

Local Presentation of Substrate Molecules Directs Axon Specification by Cultured Hippocampal Neurons

1999

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Axon specification is a crucial, early step in neuronal development, but little is known about how this event is controlled in vivo. To test the hypothesis that local presentation of growthpromoting molecules can direct axon specification, we cultured hippocampal neurons on substrates patterned with stripes of poly-L-lysine and either laminin (LN) or the neuron-glia cell adhesion molecule (NgCAM). Although undifferentiated neurites contacted both substrates equally, axons formed preferentially on LN or NgCAM. Time-lapse studies revealed that changes in the growth pattern of a cell indicative of axon specification began almost immediately after the growth cone of one of the neurites of the cell contacted LN or NgCAM. When cells were plated on alternating stripes of LN and NgCAM, cells with their somata on LN usually formed axons on NgCAM, whereas those with somata on NgCAM preferentially formed axons on LN. This suggests that the change from one axon-promoting substrate to another also provides a signal sufficient to specify the axon. These results demonstrate that contact with preferred substrate molecules can govern which neurite becomes the axon and thus direct the development of neuronal polarity.

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Growth and Differentiation of Neural Cells in Defined Media

Cited by 100 publications

References 127 publications

Migration of Neurotrophic Factors-Secreting Mesenchymal Stem Cells Toward a Quinolinic Acid Lesion as Viewed by Magnetic Resonance Imaging

Migration of Neurotrophic Factors-Secreting Mesenchymal Stem Cells Toward a Quinolinic Acid Lesion as Viewed by Magnetic Resonance Imaging

The mouse neuronal cell surface protein F3: a phosphatidylinositol-anchored member of the immunoglobulin superfamily related to chicken contactin.

Local Presentation of Substrate Molecules Directs Axon Specification by Cultured Hippocampal Neurons

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