Growth and differentiation of human induced pluripotent stem cell (hiPSC)-derived kidney organoids using fully synthetic peptide hydrogels

Treacy, Niall J.; Clerkin, Shane; Davis, Jessica L.; Kennedy, Ciarán; Miller, Aline F.; Saiani, Alberto; Wychowaniec, Jacek K.; Brougham, Dermot F.; Crean, John

doi:10.1016/j.bioactmat.2022.08.003

Cited by 16 publications

(39 citation statements)

References 114 publications

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“…This generalization of our approach could be the solution to overcome several challenges in the field of organoids, embryoids or even tissue engineering (Huch et al, 2017; Laurent et al, 2017). CartoCell and the Single-cell Cartography methodology can unveil hidden patterns in a simple and visual manner, what is pivotal to improve the study of the self-organization of complex epithelial tissues where cells are in close contact with each other (Carleton et al, 2022; Dahl-Jensen & Grapin-Botton, 2017; Metzger et al, 2018; Takasato et al, 2016; Treacy et al, 2023). In a biomedical context, the possibility of analyzing a very large number of samples is ideal for testing the reproducibility of epithelial organoids cultures and performing detailed comparisons between physiological and pathological conditions.…”

Section: Discussionmentioning

confidence: 99%

“…CartoCell and the Single-cell Cartography methodology can unveil hidden patterns in a simple and visual manner, which is pivotal to improve the study of the self-organization of complex epithelial tissues where cells are in close contact with each other 57,58,75,78,79 . In a biomedical context, the possibility of analyzing a very large number of samples is ideal for testing the reproducibility of epithelial organoids cultures and performing detailed comparisons between physiological and pathological conditions.…”

Section: Discussionmentioning

confidence: 99%

“…The copyright holder for this preprint this version posted January 6, 2023. ; https://doi.org/10. 1101/2023 In this article, we image, process, and analyze whole Madin-Darby Canine Kidney (MDCK) 3D epithelial cell cultures. Despite their simplicity, these cysts have previously been used as a suitable model system to study the establishment of cell polarity and cell junctions (Bryant & Mostov, 2008;Dukes et al, 2011;Martín-Belmonte et al, 2008;Vidal-Quadras et al, 2017;Yonemura, 2014), epithelial morphogenesis and physiology (Alfonso-Pérez et al, 2022;Guo et al, 2008;Imai et al, 2015;O'Brien et al, 2002;Wells et al, 2013;Yu et al, 2007), tumor progression (Fessenden et al, 2018;Sakurai et al, 2012;Schmeichel & Bissell, 2003;L.…”

Section: Introductionmentioning

confidence: 99%

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CartoCell, a high-content pipeline for 3D image analysis, unveils cell morphology patterns in epithelia

Román

Gordillo-Vázquez

Franco-Barranco

et al. 2023

Preprint

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Decades of research have not yet fully explained the mechanisms of epithelial self organization and 3D packing. Single-cell analysis of large 3D epithelial libraries is crucial for understanding the assembly and function of whole tissues. Combining 3D epithelial imaging with advanced deep learning segmentation methods is essential for enabling this high-throughput analysis. We introduce CartoCell, a deep learning-based pipeline that uses small datasets to generate accurate labels for hundreds of whole 3D epithelial cysts. Our method detects the realistic morphology of epithelial cells and their contacts in the 3D structure of the tissue. CartoCell enables the quantification of geometric and packing features at the cellular level. Our Single-cell Cartography approach then maps the distribution of these features on 2D plots and 3D surface maps, revealing cell morphology patterns in epithelial cysts.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

CartoCell, a high-content pipeline for 3D image analysis, unveils cell morphology patterns in epithelia

Román

Gordillo-Vázquez

Franco-Barranco

et al. 2023

Preprint

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“…Several studies have effectively used single cell technologies to profile human iPSC‐derived organoids and murine tumor organoids in complex 3D in vitro models, highlighting the relevance and feasibility of this approach. [ 78,158,159,205–209 ] In one notable example, Qin et al. created a co‐culture model of murine CRC organoids with colonic fibroblasts and macrophages in Matrigel domes and used CyTOF to explore the effect of oncogenic mutations and microenvironmental cues on cancer cell signaling mediated by post‐translational modifications.…”

Section: Elucidating Microenvironmental and Tumor Cell Heterogeneity ...mentioning

confidence: 99%

“…Several studies have effectively used single cell technologies to profile human iPSC-derived organoids and murine tumor organoids in complex 3D in vitro models, highlighting the relevance and feasibility of this approach. [78,158,159,[205][206][207][208][209] In one notable example, Qin et al created a co-culture model of murine CRC organoids with colonic fibroblasts and macrophages in Matrigel domes and used CyTOF to explore the effect of oncogenic mutations and microenvironmental cues on cancer cell signaling mediated by posttranslational modifications. [205] Thus, extending single cell analysis to complex PDO-based models will undoubtedly improve our characterization of the heterogeneous cell types present in 3D in vitro systems and our understanding of cell-cell and cell-TME interactions.…”

Section: Single Cell Analysis Of Pdo-based 3d In Vitro Models Of the Tmementioning

confidence: 99%

Exploring New Dimensions of Tumor Heterogeneity: The Application of Single Cell Analysis to Organoid‐Based 3D In Vitro Models

Landon‐Brace,

Li,

McGuigan

2023

Adv Healthcare Materials

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Modelling the heterogeneity of the tumor microenvironment (TME) in vitro is essential to investigating fundamental cancer biology and developing novel treatment strategies that holistically address the factors affecting tumor progression and therapeutic response. Thus, the development of new tools for both in vitro modelling, such as patient‐derived organoids (PDOs) and complex 3D in vitro models, and single cell omics analysis, such as single‐cell RNA‐sequencing, represents a new frontier for investigating tumor heterogeneity. Specifically, the integration of PDO‐based 3D in vitro models and single cell analysis offers a unique opportunity to explore the intersecting effects of interpatient, microenvironmental, and tumor cell heterogeneity on cell phenotypes in the TME. In this review, we discuss the current use of PDOs in complex 3D in vitro models of the TME and highlight emerging directions in the development of these models. Next, we examine work that has successfully applied single cell analysis to PDO‐based models and identify important experimental considerations for this approach. Finally, we highlight open questions that may be amenable to exploration using the integration of PDO‐based models and single cell analysis. Ultimately, such investigations may facilitate the identification of novel therapeutic targets for cancer that address the significant influence of tumor‐TME interactions.This article is protected by copyright. All rights reserved

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Self‐Assembled Peptide Hydrogels Loaded with Umbilical Cord‐Derived Mesenchymal Stem Cells Repairing Injured Endometrium and Restoring Fertility

Lv,

Niu,

Zhang

et al. 2024

Adv Healthcare Materials

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Endometrial injury is a major cause of infertility and recurrent miscarriage. However, no clinically available methods currently exist to effectively repair the damaged endometrium. Mesenchymal stem cells (MSCs) have emerged as a promising therapeutic approach for promoting tissue regeneration, yet a biocompatible scaffold capable of delivering MSCs and supporting their growth is needed. Herein, the study reports a peptide hydrogel scaffold, self‐assembled from a peptide IVK8‐RGD consisting of an ionic complementary peptide sequence IEVEIRVK and a bioactive sequence RGD, to load umbilical cord‐derived mesenchymal stem cells (UC‐MSCs). This peptide forms a hydrogel under the physiological condition through self‐assembly, and the peptide hydrogel exhibits injectability and adhesiveness to uterus, making it suitable for endometrial repair. Importantly, this hydrogel supports the adhesion and proliferation of UC‐MSCs in a 3D environment. In vivo experiments using rats with endometrial injury have shown that treatment with IVK8‐RGD hydrogel loaded with UC‐MSCs effectively restores endometrial thickness, inhibits fibrosis, and facilitates angiogenesis through activating Raf/MEK/ERK pathway, leading to significantly improved fertility and live birth rate. These findings demonstrate the potential of the UC‐MSCs‐loaded hydrogel in repairing damaged endometrium and may address the unmet clinical needs of treating recurrent miscarriage and infertility induced by endometrial damage.

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Growth and differentiation of human induced pluripotent stem cell (hiPSC)-derived kidney organoids using fully synthetic peptide hydrogels

Cited by 16 publications

References 114 publications

CartoCell, a high-content pipeline for 3D image analysis, unveils cell morphology patterns in epithelia

CartoCell, a high-content pipeline for 3D image analysis, unveils cell morphology patterns in epithelia

Exploring New Dimensions of Tumor Heterogeneity: The Application of Single Cell Analysis to Organoid‐Based 3D In Vitro Models

Self‐Assembled Peptide Hydrogels Loaded with Umbilical Cord‐Derived Mesenchymal Stem Cells Repairing Injured Endometrium and Restoring Fertility

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