Growth and cytopathology of Mycoplasma synoviae in chicken embryo cell cultures

Aldridge, Kenneth E.

doi:10.1128/iai.12.1.198-204.1975

Cited by 14 publications

(7 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Attachment of M. synoviae cells to erythrocytes (25,26) and chicken embryo cells (1,2) has been documented, and available evidence indicates a direct correlation between hemad-sorption of erythrocytes to colonies of different clones of M. synoviae WVU-1853 and their hemagglutination activity (26). Furthermore, clonal variation in hemadsorption and hemagglutination of M. synoviae strains has been reported both in vitro and after passage in vivo (26), suggesting the occurrence of phase variation in the expression of proteins involved in these processes.…”

mentioning

confidence: 99%

Mycoplasma synoviae has two distinct phase-variable major membrane antigens, one of which is a putative hemagglutinin

et al. 1997

View full text Add to dashboard Cite

Mycoplasma synoviae is a major pathogen of poultry, causing synovitis and respiratory infection. A cluster of 45-to 50-kDa membrane proteins is immunodominant in strain WVU-1853. Four distinct proteins were identified in this cluster by high-pressure liquid chromatography. Monoclonal antibodies and monospecific antisera against each established that they fell into two groups, MSPA and MSPB, each containing two members distinguishable by a difference in hydrophobicity. A 25-to 30-kDa membrane protein (MSPC) was shown to be antigenically related to the MSPB proteins. Considerable variation in the size and expression of MSPA and MSPB was observed among different strains of M. synoviae. Examination of expression in colonies of strain WVU-1853 established that both MSPA and MSPB (and MSPC) were phase variable. Immunostaining of MSPB (and MSPC) with monoclonal antibodies exhibited quantal variation, with three distinct levels observed between and within colonies. Hemadsorption by M. synoviae colonies was also found to be phase variable, with some colonies exhibiting sectorial expression of hemadsorption. Monospecific antisera against MSPA inhibited hemagglutination, but neither monoclonal antibodies nor monospecific antisera against MSPB could inhibit hemagglutination. However, loss of the capacity to hemadsorb by individual clones was associated with loss of expression of both MSPA and MSPB. These findings have elucidated the complexity of structure, function, and expression of the 45-to 50-kDa membrane protein cluster of M. synoviae, and they suggest that all members of the cluster may be involved in adhesion.

show abstract

mentioning

confidence: 99%

Mycoplasma synoviae has two distinct phase-variable major membrane antigens, one of which is a putative hemagglutinin

et al. 1997

View full text Add to dashboard Cite

show abstract

“…It is of interest that there does not appear to be a consistent correlation between the ability of mycoplasmas to adsorb to mammalian cells (19) and their ability to in- duce interferon in sheep leukocyte cultures. Both M. pneumoniae and M. synoviae, however, were the most consistent inducers of interferon in human lymphocytes and both have been shown to have a high avidity for various cells (1,29). Although viruses are capable of inducing interferon in a variety of cell types, the present evidence indicates that mycoplasma-and mitogen-associated induction (16) is restricted to leukocytes and, more specifically lymphocytes.…”

Section: Interferon Induction By Ovine Leukocytesmentioning

confidence: 54%

“…With the exception of M. synoviae all mycoplasmas were grown in Mycoplasma broth or agar (Difco) supplemented to final concentrations of 15 to 20% (vol/vol) horse serum, 5% (vol/vol) fresh yeast extract, and 1,000 units of penicillin G per ml (5,12). M. synoviae was grown as described by Aldridge (1). Mycoplasma pools were prepared and quantitated as previously described (11).…”

mentioning

confidence: 99%

Induction of interferon in ovine and human lymphocyte cultures by mycoplasmas

Cole¹,

Overall²,

Lombardi³

et al. 1976

Infect Immun

View full text Add to dashboard Cite

Mycoplasma pneumoniae, Acholeplasma laidlawii, M. arthritidis, and M. pulmonis were shown to induce interferon in the lymphocyte fraction of ovine peripheral blood leukocytes, but not in the polymorphonuclear leukocyte fraction. Human peripheral blood lymphocytes produced significant levels of interferon in response to infection with M. pneumoniae and M. synoviae. The antiviral substance induced by the mycoplasmas in human lymphocytes was characterized as interferon by the usual criteria. 93

show abstract

“…Unfortunately, some difficulties can be encountered in the use of tracheal organ culture because of the complexity of cell types, mucus production, the small mass of tissue per explant, and a relatively high degree of nonspecific attachment. Monolayer cell cultures (5, 12), and particularly fibroblasts (2,3,22), are valuable models for mycoplasma host interactions. We have conducted an analysis of human lung fibroblast cell cultures as a potential in vitro model for M. pneumoniae infection.…”

mentioning

confidence: 99%

Interaction of Mycoplasma pneumoniae with human lung fibroblasts: characterization of the in vitro model

Gabridge¹,

Taylor‐Robinson²,

Davies³

et al. 1979

Infect Immun

View full text Add to dashboard Cite

The interaction of pathogenic Mycoplasma pneumoniae and host cells was studied in cell cultures of MRC-5 human lung fibroblasts. A comparison of results obtained with fibroblasts in a monolayer format and with hamster tracheal explant cultures indicated that the former can bind significantly larger numbers of mycoplasmas. In addition, the attachment was 96% specific, that is, mediated through a neuraminidase-sensitive receptor on the host cell. Uptake of mycoplasmas was directly related to the number of mycoplasma cells present in the inoculum, and attachment was virtually complete within a 30-min period at 37 degrees C. High doses of M. pneumoniae induced a marked cytopathic effect, whereas doses of less than or equal to 10(6) colony-forming units per ml produced grossly observable cell damage that was moderate and variable. Transmission electron microscopy studies indicated that attachment of M. pneumoniae to the surface of lung fibroblasts occurred with the specialized terminal structure or binding site oriented closest to the epithelial cell surface. The filamentous mycoplasma cells were spatially arranged in several configurations and were not limited to a vertical orientation. The advantages and disadvantages of human lung fibroblast monolayer cultures, in reference to other in vitro models are discussed. A new mycoplasma agar medium (G-200 agar) with a defined tissue culture base and 10% horse serum is also described.

show abstract

Growth and cytopathology of Mycoplasma synoviae in chicken embryo cell cultures

Cited by 14 publications

References 11 publications

Mycoplasma synoviae has two distinct phase-variable major membrane antigens, one of which is a putative hemagglutinin

Mycoplasma synoviae has two distinct phase-variable major membrane antigens, one of which is a putative hemagglutinin

Induction of interferon in ovine and human lymphocyte cultures by mycoplasmas

Interaction of Mycoplasma pneumoniae with human lung fibroblasts: characterization of the in vitro model

Contact Info

Product

Resources

About