Growth and Cytology of Callus and Cell Suspension Cultures of Vicia faba L.

Röper, W.

doi:10.1016/s0044-328x(79)80202-0

Cited by 13 publications

(5 citation statements)

References 14 publications

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“…The influence of media composition and explant source on the initiation and maintenance of cultures were tested and the conditions optimized for maximum increase in callus fresh weight. The morphogenic potential of faba bean cells cultured in vitro was low [146]. To develop a system for plant regeneration from single cells, callus and cell suspension cultures were established over a long period of time, but all attempts to initiate shoot regeneration remained unsuccessful.…”

Section: In Vitro Regenerationmentioning

confidence: 99%

Impact of Molecular Technologies on Faba Bean (Vicia faba L.) Breeding Strategies

Gnanasambandam¹,

Paull

Torres

et al. 2012

Agronomy

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Faba bean (Vicia faba L.) is a major food and feed legume because of the high nutritional value of its seeds. The main objectives of faba bean breeding are to improve yield, disease resistance, abiotic stress tolerance, seed quality and other agronomic traits. The partial cross-pollinated nature of faba bean introduces both challenges and opportunities for population development and breeding. Breeding methods that are applicable to self-pollinated crops or open-pollinated crops are not highly suitable for faba bean. However, traditional breeding methods such as recurrent mass selection have been established in faba bean and used successfully in breeding for resistance to diseases. Molecular breeding strategies that integrate the latest innovations in genetics and genomics with traditional breeding strategies have many potential applications for future faba bean cultivar development. Hence, considerable efforts have been undertaken in identifying molecular markers, enriching genetic and genomic resources using high-throughput sequencing technologies and improving genetic transformation techniques in faba bean. However, the impact of research on practical faba bean breeding and cultivar release to farmers has been limited due to disconnects between research and breeding objectives and the high costs of research and implementation. The situation with faba bean is similar to other small crops and highlights the need for coordinated, collaborative research programs that interact closely with commercially focused breeding programs to ensure that technologies are implemented effectively

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Section: In Vitro Regenerationmentioning

confidence: 99%

Impact of Molecular Technologies on Faba Bean (Vicia faba L.) Breeding Strategies

Gnanasambandam¹,

Paull

Torres

et al. 2012

Agronomy

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“…Dans de nombreux cas, une analyse caryologique de ces cultures a Ct C faite (20,2,12,15). Par contre, la nCofornation de tiges ne semblait pas avoir Ct C obtenue chez la fiverole.…”

Section: Introductionunclassified

Induction de l'organogénèse sur des cals de Vicia faba minor provenant d'apex

Galzy¹,

Hamoui²

1981

Can. J. Bot.

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In vitro cultivated plants of Vicia faba minor were propagated by microcutting and used as the initial material for these experiments. Shoot tips were first cultivated on callus-inducing media. The transfer of calluses to media containing lower levels of growth substances resulted, in some instances, in the neoformation of roots and shoots. Rooted plantlets were then obtained from these shoots.Auxins (α-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D)) and cytokinins (6-benzylaminopurine (6-BAP) and 6-furfurylaminopurine (kinetin)) clearly influenced the rate of callus growth and the capacity for organogenesis. They were mixed together to give four callus-inducing media, each containing an auxin (1 mg/L) and a cytokinin (5 mg/L). The media tested for organogenesis contained in each case the same growth substances as the initiation medium but the concentration of auxin and cytokinin was reduced and their ratio modified. Shoot differentiation occurred only when the auxin used was NAA. The most effective combination for shoot formation was the following: 0.1 mg/L NAA and 0.5 mg/L 6-BAP.

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“…A critical perusal of literature shows that the stability or lack of it, is an associated phe nomenon of in vitro culture systems and plantlets regenerated from them (D'Amato 1975, Salmia 1975, Sekerka 1977, Roper 1979, Bayliss 1980, Orton 1980, Constantin 1981, McCoy and Phillips 1982, McCoy et al 1982, Papes et al 1983, Sen and Sharma 1991. A number of workers have demonstrated the instability phenomenon by karyological studies on root tip apices (McCoy and Bingham 1987, Roy 1980, Browers and Orton 1982, Sree Ramulu et al 1983, Mathur et al 1987, Sen and Sharma 1991.…”

Section: Discussionmentioning

confidence: 99%