Growth and conidia production in race NG‐5/IA‐65 of <i>Pyricularia oryzae</i> Cav. <i>in vitro</i>

Awoderu, V. A.; Esuruoso, O. F.; Adeosun, O. O.

doi:10.1002/jobm.3620310302

Cited by 6 publications

(5 citation statements)

References 3 publications

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“…50 mm) and minimum colony diameter was observed in Yeast extract + 2% soluble starch (55.00 mm) (Table 4). The similar results were obtained by Awoderu et al (1991) and Getachew Gashaw et al (2014) 5). Similarly, Arunkumar and Singh (1995) reported good growth and sporulation of 2.95 µm in leaf blast and 3.00 µm in neck blast in oat meal agar followed by host extract agar media.…”

Section: Resultssupporting

confidence: 88%

Cultural and morphological studies on Ponnampet leaf and neck blast isolates of Magnaporthe grisea (Herbert) barr on rice (Oryza sativa L.)

Teli

Prasannakumar

Jyothi

et al. 2016

JANS

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The study was carried out to standardize the optimal growth, sporulation and production of perfect stage of pathogen on different media. Among different media used such as Potato dextrose Agar (PDA), Oat meal Agar, Ragi flour agar, yeast extract + 2% soluble starch, Host extract + 2% soluble sucrose agar, Potato dextrose agar + Biotin + Thiamine and Rice flour agar, Oat meal agar and potato dextrose agar was found to be best media for radial growth and sporulation of M. grisea. Maximum conidia length (9.46Î¼m) and breadth (7.36Î¼m) was recorded in Oat meal agar followed by Potato dextrose agar and least conidia length (6.15 Î¼m) and breadth (5.11 Î¼m) was recorded in ragi flour media after 20 days of inoculation. Conidial size varied in leaf and neck blast isolates, the maximum mean colony diameter of 88.00mm and 89.16mm in neck and leaf blast was recorded in Oat meal agar respectively. The maximum sporulation mean index was observed in Oat Meal agar of 3.15 Î¼m in leaf and 3.20 Î¼m in neck blast was recorded. The best growth of the pathogen was recorded at optimum pH range from 6.0 - 7.0 and temperature of 27oC. Therefore oat meal agar media was found to be best among all the media used for growth, sporulation, conidial size and colony characters of M. grisea.

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Section: Resultssupporting

confidence: 88%

Cultural and morphological studies on Ponnampet leaf and neck blast isolates of Magnaporthe grisea (Herbert) barr on rice (Oryza sativa L.)

Teli

Prasannakumar

Jyothi

et al. 2016

JANS

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“…So, 25°C and 30°C were found to be the suitable media for Pyricularia grisea (Table 2). This result is in conformity with Awoderu et al (1991), Okeke et al (1992) and Arunkumar and Singh (1995) who reported that optimum temperature for the mycelial growth of P. grisea is 25 to 30°C. This result also confirms the findings of Okeke et al (1992) who noted that the growth of P. grisea was optimum at 28°C, moderate at 23°C and minimum at 15°C and growth was inhibited at a temperature of 37°C.…”

Section: Growth Of the Pathogen In Different Temperaturessupporting

confidence: 92%

Cultural and Morphological Characterization of Pyricularia grisea Causing Blast Disease of Rice

Dutta¹,

Bandyopadhyay²,

Jha³

2019

Int.J.Curr.Microbiol.App.Sci

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Rice (Oryza sativa L.) is the world's most important crop and a primary source of food for more than half of the global population. In India, rice is a staple food for more than 65 per cent population. However, the crop is subjected to attack by many diseases among which rice blast caused by the fungus Pyricularia oryzae is one of the most economically important diseases. The pathogen was isolated from the rice field of Uttar Banga Krishi Viswavidyalaya Farm, purified, and characterized based on morphological and cultural characters. Highest mycelial growth of 28.50 mm and 71.33 mm were observed in oat meal agar (OMA) medium at 2 and 6 days after inoculation (DAI) respectively, whereas growth of 47.77 mm was observed in potato dextrose agar (PDA) medium at 4 DAI. The mycelial growth was highest at 30 0 C at 2 (27.00 mm) and 6 (72.17 mm) DAI whereas at 4 DAI, maximum growth of 43.50 mm was recorded at 25 0 C. The best nitrogen and carbon sources for the pathogen in vitro were found to be potassium nitrate and glucose, respectively whereas the best pH for the growth of the pathogen was found to be 6. The conidia in infected leaf sample were three-celled with an average size of 22.42 × 8.59 μm. The conidia on rice grain were predominantly two celled with an average size of 16.45 × 7.46 μm. Conidial production was not observed in any of the media tested including PDA, OMA. However, on rice grain, the fungus produced conidia at 55-60 days.

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“…Relative humidity (RH) of the culture environment is an important factor that induces conidia production on synthetic medium. Awoderu et al (1991) showed the effect of relative humidity (RH) on conidia production and the production was higher at RH 92.9% and lower at RH 56.8%. During the time of mycelia removal and conidia harvest, water was used to wash off mycelia and conidia, and meanwhile increased the relative humidity of the plate, which may have acted as a positive factor for the increased conidia production.…”

Section: Discussionmentioning

confidence: 99%

Suitable methods for isolation, culture, storage and identification of wheat blast fungus Magnaporthe oryzae Triticum pathotype

et al. 2020

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Wheat blast disease caused by a South American lineage of Magnaporthe oryzae Triticum (MoT) pathotype has emerged as a serious threat to wheat production in Bangladesh since its first emergence in 2016. Efficient and suitable methods for isolation, storage, inoculum production and molecular characterization of the pathogen can help in achieving the target of sustainable management of the disease in a relatively short period of time. In this study, we aimed to develop suitable methods for isolation, storage and morphological characterization and molecular identification of MoT isolates collected from the blast-infected wheat fields in Bangladesh. This process included modification of existing protocols that were available for a related fungal pathogen M. oryzae or de novo method development and validation. We developed suitable methods for isolation of MoT from field-infected plant samples using modified monoconidial isolation technique and produced abundant conidia from a single mycelial plate for in vivo pathogenicity assay in a reproducible manner. Cultural and morphological characterization of the isolates revealed that all Bangladeshi MoT isolates are of a single clonal lineage with similar cultural and morphological characters. Molecular detection of isolates with M. oryzae-specific primers Pot1 and Pot2 and MoT-specific primers MoT3F and MoT3R produced bands with the expected size from all wheat-infecting isolates. We also successfully established a PCR-based detection system based on a commercially available detection kit for field-infected leaf and seed samples by detecting Pot2- and MoT3-specific bands. Additionally, the simple method we developed in our study for producing abundant conidia in a very short period of time will be very helpful in studying biology of the wheat blast fungus. This method was also proven to be more user-friendly and cost-effective than previously available methods. Successful characterization of MoT isolates at morphological and molecular levels coupled with detection of the pathogen in infected field and seed lots should be useful for efficient surveillance and management of the fearsome wheat blast disease.

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Growth and conidia production in race NG‐5/IA‐65 of Pyricularia oryzae Cav. in vitro

Cited by 6 publications

References 3 publications

Cultural and morphological studies on Ponnampet leaf and neck blast isolates of Magnaporthe grisea (Herbert) barr on rice (Oryza sativa L.)

Cultural and morphological studies on Ponnampet leaf and neck blast isolates of Magnaporthe grisea (Herbert) barr on rice (Oryza sativa L.)

Cultural and Morphological Characterization of Pyricularia grisea Causing Blast Disease of Rice

Suitable methods for isolation, culture, storage and identification of wheat blast fungus Magnaporthe oryzae Triticum pathotype

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