Growth and cellular proliferation of pig corpora lutea throughout the oestrous cycle

Ricke, William A.; Redmer, Dale A.; Reynolds, Lawrence P.

doi:10.1530/jrf.0.1170369

Cited by 35 publications

(13 citation statements)

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“…Consistent with the more limited data of Ricke et al [39] on progesterone content and 3␤-HSD protein expression, we observed a rapid increase in progesterone production, progesterone release, and mRNA expression for StAR protein, P450 scc , and 3␤-HSD in HR and RH gilts in the 12-to 24-h period after ovulation. Our observation that progesterone content in luteal tissue was highly correlated with both oviductal and peripheral plasma progesterone concentrations further extends the data of Ricke et al [39] and suggest that because of the activity of the subovarian countercurrent transfer system in pigs [40], oviductal progesterone concentrations will reflect changes in early luteal function and exert regulatory effects on the oviduct [41]. Although Ricke et al [39] indicated that luteal progesterone content provides a good index of normal luteal function, our data suggest that progesterone production and release by luteal tissue in vitro are better measures of luteal status.…”

Section: Discussionsupporting

confidence: 92%

“…Our observation that progesterone content in luteal tissue was highly correlated with both oviductal and peripheral plasma progesterone concentrations further extends the data of Ricke et al [39] and suggest that because of the activity of the subovarian countercurrent transfer system in pigs [40], oviductal progesterone concentrations will reflect changes in early luteal function and exert regulatory effects on the oviduct [41]. Although Ricke et al [39] indicated that luteal progesterone content provides a good index of normal luteal function, our data suggest that progesterone production and release by luteal tissue in vitro are better measures of luteal status. For example, progesterone production and release in tissue recovered from the HRϩI group was higher than in HR and RH groups, whereas luteal tissue progesterone content did not differ among the three treatments.…”

Section: Discussionsupporting

confidence: 91%

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Feed Restriction and Insulin Treatment Affect Subsequent Luteal Function in the Immediate Postovulatory Period in Pigs: Progesterone Production In Vitro and Messenger Ribonucleic Acid Expression for Key Steroidogenic Enzymes1

Mao¹,

Treacy²,

Almeida³

et al. 2001

Biology of Reproduction

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Progesterone production and release in vitro, and mRNA expression for key steroidogenic enzymes, were studied in luteal tissue recovered in the immediate postovulatory period from cyclic gilts allocated to one of three treatments: moderate feed restriction during the first (RH) or second week of the estrous cycle, with (HR+I) or without (HR) concomitant injections of long-acting insulin. Time of feed restriction affected neither progesterone production or release, nor mRNA expression for several key steroidogenic enzymes. However, luteal tissue from RH but not from HR gilts responded to LH stimulation by increasing progesterone production and release (P: < 0.05). Insulin treatment increased progesterone production and release, restored luteal tissue responsiveness to LH, up-regulated steroidogenic enzyme mRNA expression, and down-regulated the tissue inhibitor of metalloproteinase-I mRNA expression in HR+I compared with HR gilts (P: < 0.05). In vitro progesterone production and gene expression were affected by time of tissue collection after ovulation in RH and HR gilts but not in HR+I gilts, and were correlated with temporal changes in oviductal and peripheral plasma progesterone concentrations. Inherent differences in luteal function therefore appear to mediate latent effects of nutrition and insulin treatment on circulating progesterone concentrations in the critical postovulatory period in gilts.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 91%

Feed Restriction and Insulin Treatment Affect Subsequent Luteal Function in the Immediate Postovulatory Period in Pigs: Progesterone Production In Vitro and Messenger Ribonucleic Acid Expression for Key Steroidogenic Enzymes1

Mao¹,

Treacy²,

Almeida³

et al. 2001

Biology of Reproduction

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“…This might be due not only to luteal cells proliferation [39], but also to endothelial cells proliferation (1). Also, in the cow, ewe and sow, DNA content and rate of DNA synthesis increased linearly, from early CL until the mature CL was formed [1,40,41]. The decrease in both microvascular areas and DNA content in Late-CL and regressed luteal structure in the present study suggests that blood vessel regression is associated with tissue involution that occurs during regression of the luteal structures [35,42].…”

Section: Discussionmentioning

confidence: 99%

Microvascularization and angiogenic activity of equine corpora lutea throughout the estrous cycle

Ferreira‐Dias¹,

Pinto‐Bravo²,

Mateus³

et al. 2006

Domestic Animal Endocrinology

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Corpus luteum growth and endocrine function are closely dependent on the formation of new capillaries. The objectives of this study were to evaluate (i) tissue growth and microvascular development in the equine cyclic luteal structures; (ii) in vitro angiogenic activity of luteal tissues in response to luteotrophic (LH, PGE 2 ) and luteolytic (PGF 2␣ ) hormones and (iii) to relate data to luteal endocrinological function. Our results show that microvascular density was increased in the early and mid luteal phase, followed by a fall in the late luteal phase and a further decrease in the corpus albicans. Hyperplasia of luteal tissue increased until the mid luteal phase and it was followed by tissue regression. Luteal explants were cultured with no hormone added, or with PGF 2␣ , LH, PGE 2 , LH + PGE 2 or LH + PGF 2␣ . Media conditioned by equine luteal tissue from different stages of the luteal phase were able to stimulate mitogenesis of bovine aortic endothelial cells (BAEC), suggesting the presence of angiogenic activity. No difference was observed among luteal structures on their mitogenic capacity, for any treatment used. Nevertheless, Late-CL conditioned-media with PGF 2␣ showed a significant decrease in BAEC proliferation (p < 0.05) and LH + PGF 2␣ a tendency to reduce mitogenesis. Thus, prostaglandin F 2␣ may play a role on vascular regression of the CL during the late luteal phase in the * Corresponding author. Tel.: +351 213652859; fax: +351 213652889. -mail address: gmlfdias@fmv.utl.pt (G. Ferreira-Dias Ferreira-Dias et al. / Domestic Animal Endocrinology 30 (2006) [247][248][249][250][251][252][253][254][255][256][257][258][259] mare. These data suggest that luteal angiogenesis and vascular regression in the mare are coordinated with the development of non-vascular tissue and might be regulated by many different factors. E

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“…Indeed, the administration of P 4 antagonist molecule, RU486, significantly decreased VEGF synthesis in vivo , in rat ovary [43] and in monkey endometrium [37]. Even if P 4 influence on ovarian blood flow was demonstrated [44]–[47], during the periovulatory stage, when the LH surge induces a complete inversion of follicular steroid secretion [8], the role exerted by steroids on follicular VEGF synthesis still remains to be investigated.…”

Section: Introductionmentioning

confidence: 99%

Effect of Antiprogesterone RU486 on VEGF Expression and Blood Vessel Remodeling on Ovarian Follicles before Ovulation

et al. 2014

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BackgroundThe success of ovarian follicle growth and ovulation is strictly related to the development of an adequate blood vessel network required to sustain the proliferative and endocrine functions of the follicular cells. Even if the Vascular Endothelial Growth Factor (VEGF) drives angiogenesis before ovulation, the local role exerted by Progesterone (P4) remains to be clarified, in particular when its concentration rapidly increases before ovulation.AimThis in vivo study was designed to clarify the effect promoted by a P4 receptor antagonist, RU486, on VEGF expression and follicular angiogenesis before ovulation, in particular, during the transition from pre to periovulatory follicles induced by human Chorionic Gonadotropins (hCG) administration.Material and MethodsPreovulatory follicle growth and ovulation were pharmacologically induced in prepubertal gilts by combining equine Chorionic Gonadotropins (eCG) and hCG used in the presence or absence of RU486. The effects on VEGF expression were analyzed using biochemical and immunohistochemical studies, either on granulosa or on theca layers of follicles isolated few hours before ovulation. This angiogenic factor was also correlated to follicular morphology and to blood vessels architecture.Results and ConclusionsVEGF production, blood vessel network and follicle remodeling were impaired by RU486 treatment, even if the cause-effect correlation remains to be clarified. The P4 antagonist strongly down-regulated theca VEGF expression, thus, preventing most of the angiogenic follicle response induced by hCG. RU486-treated follicles displayed a reduced vascular area, a lower rate of endothelial cell proliferation and a reduced recruitment of perivascular mural cells. These data provide important insights on the biological role of RU486 and, indirectly, on steroid hormones during periovulatory follicular phase. In addition, an in vivo model is proposed to evaluate how periovulatory follicular angiogenesis may affect the functionality of the corpus luteum (CL) and the success of pregnancy.

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Growth and cellular proliferation of pig corpora lutea throughout the oestrous cycle

Abstract: Corpora lutea were obtained from gilts on days 2, 4, 8,12,15

Cited by 35 publications

References 4 publications

Feed Restriction and Insulin Treatment Affect Subsequent Luteal Function in the Immediate Postovulatory Period in Pigs: Progesterone Production In Vitro and Messenger Ribonucleic Acid Expression for Key Steroidogenic Enzymes1

Feed Restriction and Insulin Treatment Affect Subsequent Luteal Function in the Immediate Postovulatory Period in Pigs: Progesterone Production In Vitro and Messenger Ribonucleic Acid Expression for Key Steroidogenic Enzymes1

Microvascularization and angiogenic activity of equine corpora lutea throughout the estrous cycle

Effect of Antiprogesterone RU486 on VEGF Expression and Blood Vessel Remodeling on Ovarian Follicles before Ovulation

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