Growth and biosynthetic profiles of callus and suspension cell cultures of two rare foxglove species, Digitalis grandiflora Mill. and D. ciliata Trautv.

Tomilova, S. V.; Кочкин, Д. В.; Tyurina, T. M.; Глаголева, Е.С.; Labunskaya, E. A.; Galishev, B. A.; Носов, А. М.

doi:10.1007/s11240-022-02271-y

Cited by 5 publications

(4 citation statements)

References 39 publications

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“…The tea callus (150 mg FW each) was dried in the thermostat BD-115 (Binder, Tuttlingen, Germany) to a constant weight (70 °C, 48 h). Calculation of water amount and dry weight was carried out using the standard methods [ 56 , 57 ].…”

Section: Methodsmentioning

confidence: 99%

Changes in the Antioxidant Potential of Camellia sinensis Cultures under the Influence of Phenolic Precursors

Aksenova,

Nechaeva,

Goncharuk

et al. 2024

Molecules

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The viability, productivity and survival of higher plants under the adverse factors influence are largely determined by the functional activity of the antioxidant system. The aim of our work was to investigate changes in formation of high-molecular (superoxide dismutase and peroxidase) and low-molecular (phenolics, including flavanols and proanthocyanidins) antioxidants in callus culture of Camellia sinensis under influence of phenolic precursors (L-phenylalanine—3 mM, trans-cinnamic acid—1 mM, naringenin—0.5 mM). According to the data obtained, the effect of precursors on tea callus cultures did not lead to significant increasing of superoxide dismutase and peroxidase activity in most cases. However, it led to the increased accumulation of the total phenolics content, as well as flavanols and proanthocyanidins contents. For C. sinensis callus cultures, the most promising regulator of phenolic compounds was L-phenylalanine, in the presence of which its content increased almost twice. Thus, the exogenous effect of various precursors is possible to use for the targeted regulation of certain phenolics classes accumulation in plant cells.

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Section: Methodsmentioning

confidence: 99%

Changes in the Antioxidant Potential of Camellia sinensis Cultures under the Influence of Phenolic Precursors

Aksenova,

Nechaeva,

Goncharuk

et al. 2024

Molecules

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show abstract

“…The tea callus was dried in the thermostat BD-115 (Binder, Tuttlingen, Germany) to a constant weight (70 °C, 48 h). Calculation of water amount was carried out using the standard formula [ 70 ].…”

Section: Methodsmentioning

confidence: 99%

Influence of Different Precursors on Content of Polyphenols in Camellia sinensis In Vitro Callus Culture

Aksenova

Nechaeva

Zubova

et al. 2023

Plants

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Plant tissue cultures are considered as potential producers of biologically active plant metabolites, which include various phenolic compounds that can be used to maintain human health. Moreover, in most cases, their accumulation is lower than in the original explants, which requires the search for factors and influences for the intensification of this process. In this case, it is very promising to use the precursors of their biosynthesis as potential “regulators” of the various metabolites’ formation. The purpose of our research was to study the effect of L-phenylalanine (PhA, 3 mM), trans-cinnamic acid (CA, 1 mM) and naringenin (NG, 0.5 mM), as components of various stages of phenolic metabolism, on accumulation of various phenolic compound classes, including phenylpropanoids, flavans and proanthocyanidins, as well as the content of malondialdehyde in in vitro callus culture of the tea plant (Camellia sinensis L.). According to the data obtained, the precursors’ influence did not lead to changes in the morphology and water content of the cultures. At the same time, an increase in the total content of phenolic compounds, as well as phenylpropanoids, flavans and proanthocyanidins, was noted in tea callus cultures. Effectiveness of precursor action depends on its characteristics and the exposure duration, and was more pronounced in the treatments with PhA. This compound can be considered as the most effective precursor regulating phenolic metabolism, contributing to a twofold increase in the total content of phenolic compounds, flavanes and proanthocyanidins, and a fourfold increase in phenylpropanoids in tea callus cultures.

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“…To determine the water content, 150 mg plant material samples were placed in glass weighing bottles and dried to a constant weight at 70 °C in the thermostat BD-115 (Binder, Tuttlingen, Germany) [ 75 ].…”

Section: Methodsmentioning

confidence: 99%

Effects of Hydrogen Peroxide on In Vitro Cultures of Tea (Camellia sinensis L.) Grown in the Dark and in the Light: Morphology, Content of Malondialdehyde, and Accumulation of Various Polyphenols

et al. 2022

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Tea plants (Camellia sinensis L.) are phenol-accumulating crops that are widely used for public health. The healing effect of tea leaf products is due to the biosynthesis of such phenolic compounds (PCs) as flavans, which have P-vitamin capillary-strengthening activity. Due to their limited habitat and the value of their specialized metabolites of a phenolic nature, a promising approach is to establish in vitro cultures from them that retain the ability to form PCs, which is characteristic of ex vivo tea plants. The aim of this study was to investigate the effect of exogenic H2O2 (0.01 mM; 0.1 mM; 1 mM) on the growth, morphology, degree of stress response, and accumulation of various phenolic compounds in tea plant callus cultures of different ages (24 or 36 days) grown under different cultivation conditions (darkness or light). According to the results obtained, the H2O2 effect on tea callus cultures of different ages did not cause changes in their morphophysiological characteristics, both after 2 h of exposure (rapid response of callus culture, RRCC) and after 48 h (delayed response of callus culture, DRCC). The determination of the malondialdehyde (MDA) content, which serves as an indicator of changes in the level of lipid peroxidation (LPO) and the presence of stress responses in plant cells, indicated either its maintenance at the control level, a decrease, or an increase. All these effects depended on the growth conditions of the tea callus cultures (darkness or light), their age, the duration of exposure (rapid or delayed response), and the H2O2 concentration. Similar trends were noted for the total content of PCs as well as the amount of flavans, proanthocyanidins (soluble and insoluble forms), and lignin. The plant cell responses reflected changes in its adaptation programs, when specialized metabolites act as a target for the action of H2O2, thereby contributing to an increase in their resistance.

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Growth and biosynthetic profiles of callus and suspension cell cultures of two rare foxglove species, Digitalis grandiflora Mill. and D. ciliata Trautv.

Cited by 5 publications

References 39 publications

Changes in the Antioxidant Potential of Camellia sinensis Cultures under the Influence of Phenolic Precursors

Changes in the Antioxidant Potential of Camellia sinensis Cultures under the Influence of Phenolic Precursors

Influence of Different Precursors on Content of Polyphenols in Camellia sinensis In Vitro Callus Culture

Effects of Hydrogen Peroxide on In Vitro Cultures of Tea (Camellia sinensis L.) Grown in the Dark and in the Light: Morphology, Content of Malondialdehyde, and Accumulation of Various Polyphenols

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