Abstract:Scenedesmus is a genus of microalgae employed for several industrial uses. Industrial cultivations are performed in open ponds or in closed photobioreactors (PBRs). In the last years, a novel type of PBR based on immobilized microalgae has been developed termed porous substrate photobioreactors (PSBR) to achieve significant higher biomass density during cultivation in comparison to classical PBRs. This work presents a study of the growth of Scenedesmus vacuolatus in a Twin Layer System PSBR at different light … Show more
“…Two studies involving similar systems that achieved higher biomass surface productivity of 31.2 g/m 2 /day with Halochlorella rubescens 17 and 29 g/m 2 /day with Scenedesmus vacuolatus 22 grown in twin-layer PBRs have been reported. The growth conditions for the respective studies were light intensity and CO 2 concentration of 1023 μE/m 2 /s and 3%, and 600 μE/m 2 /s and 2%, both with a light/dark cycle of 14/10 hours.…”
Section: Resultsmentioning
confidence: 99%
“… 22 calculated the photosynthetic efficiency based on the equation reported by Schultze, L. K., et al . 17 , which is expressed in terms of biomass produced in grams per moles of photons exposed in unit of Einstein (E), and compared the results with those in other studies involving similar systems 17 , 22 . The photosynthetic efficiency of the two highest biomass surface productivities of H. rubescens and S. vacuolatus previously mentioned were 0.6 and 0.95 g/E, respectively.…”
Attached cultivation systems have been receiving extensive attention as a breakthrough in microalgae cultivation technology. However, there is a lack of studies that emphasize precise optimization of important parameters in attached cultivation of microalgae. In this study, the effects of two major environmental parameters in photoautotrophic cultivation, light intensity and CO2 concentration, on the biomass and lipid surface productivity of Ettlia sp. YC001 were optimized by employing Response Surface Methodology (RSM) and validated experimentally. The optimum initial conditions for attached cultivation were use of seed from the late exponential phase (LE) and an inoculum surface density of 2.5 g/m2. By optimization, maximum biomass surface productivity of 28.0 ± 1.5 g/m2/day was achieved at 730 μE/m2/s with 8% CO2. The maximum lipid surface productivity was 4.2 ± 0.3 g/m2/day at 500 μE/m2/s with 7% CO2. Change of the fatty acid composition with respect to changes in environment parameters led to improvement of biodiesel quality at higher light intensity and higher CO2 concentration. Attached cultivation of Ettlia sp. YC001 has successfully produced biomass and lipids at a high production rate with relatively low light energy demand and high CO2 utilization.
“…Two studies involving similar systems that achieved higher biomass surface productivity of 31.2 g/m 2 /day with Halochlorella rubescens 17 and 29 g/m 2 /day with Scenedesmus vacuolatus 22 grown in twin-layer PBRs have been reported. The growth conditions for the respective studies were light intensity and CO 2 concentration of 1023 μE/m 2 /s and 3%, and 600 μE/m 2 /s and 2%, both with a light/dark cycle of 14/10 hours.…”
Section: Resultsmentioning
confidence: 99%
“… 22 calculated the photosynthetic efficiency based on the equation reported by Schultze, L. K., et al . 17 , which is expressed in terms of biomass produced in grams per moles of photons exposed in unit of Einstein (E), and compared the results with those in other studies involving similar systems 17 , 22 . The photosynthetic efficiency of the two highest biomass surface productivities of H. rubescens and S. vacuolatus previously mentioned were 0.6 and 0.95 g/E, respectively.…”
Attached cultivation systems have been receiving extensive attention as a breakthrough in microalgae cultivation technology. However, there is a lack of studies that emphasize precise optimization of important parameters in attached cultivation of microalgae. In this study, the effects of two major environmental parameters in photoautotrophic cultivation, light intensity and CO2 concentration, on the biomass and lipid surface productivity of Ettlia sp. YC001 were optimized by employing Response Surface Methodology (RSM) and validated experimentally. The optimum initial conditions for attached cultivation were use of seed from the late exponential phase (LE) and an inoculum surface density of 2.5 g/m2. By optimization, maximum biomass surface productivity of 28.0 ± 1.5 g/m2/day was achieved at 730 μE/m2/s with 8% CO2. The maximum lipid surface productivity was 4.2 ± 0.3 g/m2/day at 500 μE/m2/s with 7% CO2. Change of the fatty acid composition with respect to changes in environment parameters led to improvement of biodiesel quality at higher light intensity and higher CO2 concentration. Attached cultivation of Ettlia sp. YC001 has successfully produced biomass and lipids at a high production rate with relatively low light energy demand and high CO2 utilization.
“… Fig. 1 a Twin-layer system (Twin Layer-S): alg immobilized microalgae, pcm polycarbonate membrane as a carrier for microalgae, gf glass fiber material, air membrane pump for air supply, cm culture medium (figure reproduced and modified from Carbone et al 2017a , b ); b Decanter (Decanter-LF); c A side view of Ultraflate photobioreactor (UP-B) (Gifuni et al 2018 d Tubular bioreactor (Tubular-B); e Inclined bubble column (Inclined Bubble-C) (Olivieri et al 2012) The Twin Layer System (Twin Layer-S) consisted of an immobilized photobioreactor where microalgae are inoculated on a polycarbonate disk that is attached on a hydrophilic substrate by self-adhesion, separating the algal biomass from the bulk of the medium (Nowack et al 2005 ; Melkonian and Podola 2010 ; Li et al 2017 ). The algae were placed on the Twin Layer-S only when the liquid culture achieved a sufficient density in suspension (optical density around 0.4).…”
Section: Analysis Of the Photosynthetic State Of Microalgaementioning
confidence: 99%
“… a Twin-layer system (Twin Layer-S): alg immobilized microalgae, pcm polycarbonate membrane as a carrier for microalgae, gf glass fiber material, air membrane pump for air supply, cm culture medium (figure reproduced and modified from Carbone et al 2017a , b ); b Decanter (Decanter-LF); c A side view of Ultraflate photobioreactor (UP-B) (Gifuni et al 2018 d Tubular bioreactor (Tubular-B); e Inclined bubble column (Inclined Bubble-C) (Olivieri et al 2012) …”
Section: Analysis Of the Photosynthetic State Of Microalgaementioning
In the last years, the acidothermophilic red microalga Galdieria sulphuraria has been increasingly studied for industrial applications such as wastewater treatment, recovery of rare earth elements, production of phycobilins. However, even now it is not possible an industrial cultivation of this organism because biotechnological research on G. sulphuraria and allied species is relatively recent and fragmented. Having in mind a possible scale-up for commercial applications, we have compared the growth and photosynthetic performance of G. sulphuraria in four suspended systems (Inclined bubble column, Decanter Laboratory Flask, Tubular Bioreactor, Ultra-flat plate bioreactor) and one immobilized system (Twin Layer Sytem). The results showed that G. sulphuraria had the highest growth, productivity and photosynthetic performance, when grown on the immobilized system, which also offers some economics advantages.
“…Eventually, Twin Layer-S offers also some economics advantages for mass cultures of G. sulphuraria (Carbone et al 2017a;Podola et al 2017;Pierobon et al 2018;Zhuang et al 2018). Many high costs linked to suspended cultivation systems are eliminated: for example, the biomass is harvested directly by scraping, without a preconcentration step; there are lower water consumption and space utilization.…”
In the last years, the acidothermophilic red microalga Galdieria sulphuraria has been increasingly studied for industrial applications such as wastewater treatment, recovery of rare earth elements, production of phycobilins. However, even now it is not possible an industrial cultivation of this organism because biotechnological research on G. sulphuraria and allied species is relatively recent and fragmented. Having in mind a possible scale-up for commercial applications, we have compared the growth and photosynthetic performance of G. sulphuraria in four suspended systems (Inclined bubble column, Decanter Laboratory Flask, Tubular Bioreactor, Ultra-flat plate bioreactor) and one immobilized system (Twin Layer Sytem). The results showed that G. sulphuraria had the highest growth, productivity and photosynthetic performance, when grown on the immobilized system, which also offers some economics advantages.
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