Growth and behaviour of bovine articular chondrocytes on nanoengineered surfaces: Part I

Shaik, Jameel; Mohammed, Javeed Shaikh; McShane, Michael J.; Mills, David K.

doi:10.1504/ijnt.2011.041438

Cited by 3 publications

(5 citation statements)

References 94 publications

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“…Statistical analyses of chondrocyte viability and metabolic activity results on mono-, bi-, and tri-layer nano¯lm architectures indicated the signi¯cant in°uence of cell seeding density and number of nano¯lm layers on the viability and metabolic activity of chondrocytes. 27 In the current study, we extended the above-mentioned work to examine the in°uence of di®erent multilayer nano¯lm surfaces on the phenotype of PBACs. Bright¯eld microscopy and°u orescence microscopy techniques were used to capture the morphology of chondrocytes on nanolm surfaces before and after the cells underwent LiveÀDead viability assay, respectively.…”

Section: Introductionmentioning

confidence: 92%

“…18 Multilayer nano¯lms are akin to soft hydrogels 23 and have emerged as versatile platforms for the growth of cells. 1,24À26 In a recent demonstration, 27 we studied the growth and behavior of primary bovine articular chondrocytes (PBACs) on layer-by-layer (LbL) selfassembled nano¯lms of 11 di®erent biomaterials, including poly(styrene sulfonate) (PSS),¯bronectin, poly-L-lysine (PLL), poly-D-lysine, laminin, bovine serum albumin (BSA), chondroitin sulfate, poly(ethyleneimine), poly(dimethyldiallylammonium chloride) (CPDDA), collagen and poly(ethylene glycol) amine (PEG-NH 2 ). Mono-, bi-, and trilayer nano¯lm architectures were deposited on polystyrene surfaces.…”

Section: Introductionmentioning

confidence: 98%

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Behavior of Articular Chondrocytes on Nanoengineered Surfaces

et al. 2013

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Nano LIFE 2013.03. Downloaded from www.worldscientific.com by RUTGERS UNIVERSITY on 04/11/15. For personal use only.In tissue engineering, surface modi¯cation has becomes one of the leading methods to enhance initial cell attachment and subsequent cellular growth, di®erentiation and tissue formation. This work studied growth and behavior of primary bovine articular chondrocytes on self-assembled multilayer nano¯lms composed of: polyelectrolytes [poly(styrene sulfonate) (PSS), poly-L-lysine (PLL), poly-D-lysine (PDL), chondroitin sulfate (CS), poly(ethyleneimine) (PEI), poly(dimethyldiallylammonium chloride) (PDDA), poly(ethylene glycol) amine (PEG À NH 2 )] and proteins [bovine serum albumin (BSA), collagen,¯bronectin, laminin]. These biomaterials were used to build mono-, bi-, and tri-layer nano¯lm architectures. Potential cytotoxic e®ects were assessed using Live/Dead assay and cell proliferation was quanti¯ed using MTT assay. Bright¯eld and°u orescence microscopy were used to analyze chondrocyte morphology. ImageJ software was used to analyze the number, mean area, circularity and Feret's diameter of viable cells. Cumulative results demonstrated that chondrocyte growth; proliferation and functionality were dependent on initial cell density, nano¯lm thickness and material composition of nano¯lms.

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Section: Introductionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 98%

Behavior of Articular Chondrocytes on Nanoengineered Surfaces

et al. 2013

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“…All solutions were prepared using DI water with a resistivity of 18.2 M cm (Millipore Systems, Burlington, MA), and the pH was adjusted to 7.4 (except a pH of 4 for collagen and a pH of 5 for PEG-NH 2 ). The concentrations of the different solutions were kept similar to the ones used in our previous study [13] that involved the above-mentioned biomaterials.…”

Section: Polyelectrolyte and Polypeptide Preparationmentioning

confidence: 99%

“…Different types of eukaryotic cells have been grown on LbL nanofilms and their interactions assessed [6][7][8][9][10][11][12][13][14]. Several recent studies have focused on chondrosarcoma cells and there have been divergent opinions as to the influence of the LbL films on chondrosarcoma cell behaviour.…”

Section: Introductionmentioning

confidence: 99%

In vitro evaluation of chondrosarcoma cells and canine chondrocytes on layer-by-layer (LbL) self-assembled multilayer nanofilms

et al. 2012

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Short-term cell-substrate interactions of two secondary chondrocyte cell lines (human chondrosarcoma cells, canine chondrocytes) with layer-by-layer self-assembled multilayer nanofilms were investigated for a better understanding of cellular-behaviour dependence on a number of nanofilm layers. Cell-substrate interactions were studied on polyelectrolyte multilayer nanofilms (PMNs) of eleven different biomaterials. Surface characterization of PMNs performed using AFM showed increasing surface roughness with increasing number of layers for most of the biomaterials. LDH-L and MTT assays were performed on chondrosarcoma cells and canine chondrocytes, respectively. A major observation was that 10-bilayer nanofilms exhibited lesser cytotoxicity towards human chondrosarcoma cells than their 5-bilayer counterparts. In the case of canine chondrocytes, BSA enhanced cell metabolic activity with increasing number of layers, underscoring the importance of the multilayer nanofilm architecture on cellular behaviour.

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“…Polyelectrolytes and proteins deposited through the LbL technique can be used to create either cell-resistant or cell-adhesive micropatterns. Our previous studies focused on the growth and behavior of bovine articular chondrocytes [ 30 ], human chondrosarcoma cells, and canine chondrocytes [ 31 ] on LbL-assembled nanothin films of varying configurations. We chose chondrocytes as our model cell type as they have a very plastic phenotype.…”

Section: Introductionmentioning

confidence: 99%

Chondrocyte Behavior on Micropatterns Fabricated Using Layer-by-Layer Lift-Off: Morphological Analysis

Shaik

Mohammed

McShane

et al. 2013

Journal of Medical Engineering

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Cell patterning has emerged as an elegant tool in developing cellular arrays, bioreactors, biosensors, and lab-on-chip devices and for use in engineering neotissue for repair or regeneration. In this study, micropatterned surfaces were created using the layer-by-layer lift-off (LbL-LO) method for analyzing canine chondrocytes response to patterned substrates. Five materials were chosen based on our previous studies. These included: poly(dimethyldiallylammonium chloride) (PDDA), poly(ethyleneimine) (PEI), poly(styrene sulfonate) (PSS), collagen, and chondroitin sulfate (CS). The substrates were patterned with these five different materials, in five and ten bilayers, resulting in the following multilayer nanofilm architectures: (PSS/PDDA)5, (PSS/PDDA)10; (CS/PEI)4/CS, (CS/PEI)9/CS; (PSS/PEI)5, (PSS/PEI)10; (PSS/Collagen)5, (PSS/Collagen)10; (PSS/PEI)4/PSS, (PSS/PEI)9/PSS. Cell characterization studies were used to assess the viability, longevity, and cellular response to the configured patterned multilayer architectures. The cumulative cell characterization data suggests that cell viability, longevity, and functionality were enhanced on micropatterned PEI, PSS, collagen, and CS multilayer nanofilms suggesting their possible use in biomedical applications.

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Growth and behaviour of bovine articular chondrocytes on nanoengineered surfaces: Part I

Cited by 3 publications

References 94 publications

Behavior of Articular Chondrocytes on Nanoengineered Surfaces

Behavior of Articular Chondrocytes on Nanoengineered Surfaces

In vitro evaluation of chondrosarcoma cells and canine chondrocytes on layer-by-layer (LbL) self-assembled multilayer nanofilms

Chondrocyte Behavior on Micropatterns Fabricated Using Layer-by-Layer Lift-Off: Morphological Analysis

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