Growth and activities of enzymes of primary metabolism in batch cultures of Catharanthus roseus cell suspension under different pCO2 conditions

Abstract

In vitro enzyme activities of glycolysis, pentose-phosphate pathway and dark CO2 fixation were assayed in batch cultures of heterotrophic Catharanthus roseus cells under various gassing rates and partial pressures of carbon dioxide. Detrimental effects of low pCO2 culture conditions on the growth characteristics could be linked to marked changes in levels of enzymes of primary metabolism during growth. The enzyme levels observed during the early stages of growth were found to be more stable when a constant pCO2 (20 mbar) was maintained and enabled exponential growth to be reached more rapidly.The importance of carbon dioxide as a "conditioning factor" of the culture medium is discussed.

Effects of various partial pressures of oxygen and carbon dioxide on different stages of somatic embryogenesis in Picea abies

“…Similar growth promoting effects of elevated CO 2 levels as observed here for ET of P. abies were reported for heterotrophic cell cultures of C. roseus (Ducos et al 1988). Such dark fixation of CO 2 is known to be sensitive to changes in ammonia concentration (Hammel et al 1979).…”

“…Embryogenesis from anther cultures can also be promoted by elevated pCO 2 (Johannson & Eriksson 1984). This gas, besides its positive effect on photosynthesis, also promotes growth in heterotrophic cultures of Catharanthus roseus (Ducos et al 1988).…”

Effects of Carbon Dioxide, Air Flow Rate, and Inoculation Density on the Batch Growth of Catharanthus roseus Cell Suspensions in Stirred Fermentors

“…Commercial application of plant cell culture for the production of fine chemicals is severely hampered by the inherent low specific rates of growth and product formation of the cells. Factors that have been reported to affect the growth of Catharanthus roseus cells are the inoculation density (Hegarty et al, 19861, C02 level (Maurel andPareilleux, 1985,1986;Ducos and Pareilleux, 1986;Ducos et al, 1988), air flow rate (Smart and Fowler, 1981;Maurel and Pareilleux, 1985;Ducos and Pareilleux, 1986;Hegarty et al, 19861, and initial kLa (Leckie et al, 1991). The detrimental effects of high initial kLa or high air flow rates have been attributed to the removal of key volatiles, e.g., COz (Smart and Fowler, 1981;Maurel and Pareilleux, 1985;Ducos and Pareilleux, 1986;Hegarty et al, 1986;Leckie et al, 1991).…”

Ajmalicine production by cell cultures of Catharanthus roseus: from shake flask to bioreactor

“…In literature, a positive effect on biomass growth of conditioning factors in the medium is mentioned several times (Stuart & Street 1971;Wijnsma et al 1988). There are conflicting data on the effect of carbon dioxide in the gaseous phase on the growth (Ducos & Pareilleux 1986;Ducos et al 1988;Hegarty et al 1986;Maurel & Pareilleux 1985). Taking into consideration these observations, the scale-up effects on the three aspects of productivity: biomass growth, product formation and biomass concentration were investigated.…”