Growth and Acclimation of In Vitro-Propagated M9 Apple Rootstock Plantlets under Various Visible Light Spectrums

Chung, Guem-Jae; Lee, Jin-Hui; Oh, Myung‐Min

doi:10.3390/agronomy10071017

Cited by 4 publications

(2 citation statements)

References 55 publications

(57 reference statements)

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“…Nevertheless, the acclimatization of micropropagated plants is affected by diverse in vitro environmental characteristics such as high relative humidity, less intensity of light compared to field conditions, ingredients of growing media with sucrose, and other phytohormones [82]. Even though various factors exist, light plays a key regulator which affects morphogenesis and photosynthesis which directly influence the growth and development of tissue culture-derived plants [83]. According to Woźny and Miler [84], the application of LEDs improved acclimatization of microshoots to ex vitro conditions.…”

Section: Effects Of Leds On the Growth Of Plantlets During Acclimatiz...mentioning

confidence: 99%

Light Quality-Mediated Influence of Morphogenesis in Micropropagated Horticultural Crops: A Comprehensive Overview

Fan

Manivannan

Wei

2022

BioMed Research International

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In plants, light quality plays significant roles in photomorphogenesis and photosynthesis. Efficient in vitro plant propagation techniques involve tailoring of various environmental cues and culture media according to the plant species. Plant tissue culture consists of several applications in scientific research, agriculture, biotechnology, and commercial industrial purposes. Utilization of light to enhance the quality of the in vitro raised plants have been evidenced by numerous researchers in plant tissue culture. The advent of light-emitting diode- (LED-) based artificial lighting systems in plant tissue culture for micropropagation has enhanced callus induction, shoot and root organogenesis, and acclimatization of in vitro propagated plants. Plants tend to perceive the light spectra present in the photosynthetically active region (PAR) ranging from 400 to 700 nm; this includes blue and red light wavelengths. Although the influence of spectral quality is being investigated in diverse plant species, particularly, its importance in in vitro propagated horticultural crops is gaining notable interest among researchers. In recent days, the application of LEDs provides better amenability according to the plant species of interest for efficient plant regeneration. Considering the growing necessity and emerging applications of LED supplemental lights for propagation of plants in in vitro, the present review summarizes the outcomes of various research studies dealing with LEDs in plant tissue culture. Moreover, the present endeavor has provided a comprehensive overview on the effects of LEDs in the morphogenesis of plants cultured in vitro.

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Section: Effects Of Leds On the Growth Of Plantlets During Acclimatiz...mentioning

confidence: 99%

Light Quality-Mediated Influence of Morphogenesis in Micropropagated Horticultural Crops: A Comprehensive Overview

Fan

Manivannan

Wei

2022

BioMed Research International

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“…SPAD and transpiration rate were unaffected due to the unaffected photosynthesis rate in our experiment. Chung et al [39] reported that after five weeks of transplantation, photosynthesis rate and transpiration rate of in vitro-propagated M-9 apple rootstock had a positive correlation under various visible light spectrums. Kandel [40] and Shimomoto et al [41] showed that the photosynthesis rate of a plant is positively correlated with stomatal conductance and transpiration rate.…”

Section: Effect Of Container Size (Experiments 2)mentioning

confidence: 99%

Influence of Substrate Composition and Container Size on the Growth of Tissue Culture Propagated Apple Rootstock Plants

Kim¹,

Shawon

et al. 2021

Agronomy

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Substrate composition and container size are considered crucial for apple rootstock grown in a plug seedling system. This study investigated the effect of substrate material’s proportion and different container volumes on the growth of apple rootstock (M-9) plants propagated by tissue culture. In substrate composition, three different ratios of peat moss (PM): vermiculite (VL): perlite (PL) at 1:1:1 (S1), 1:2:3 (S2), 3:1:2 (S3) were used. For container size, plants were grown in 1000 mL (C1), 500 mL (C2), and 300 mL (C3) containers filled by 1:1:1 mixture of PM, VL and PL. In both cases, plants were treated eleven weeks in a green house. Our results demonstrate that the plant height, number of leaves, leaf area, shoot fresh weight and root fresh weight of apple rootstock were significantly higher in substrate composition S3 compared with S1 composition. However, chlorophyll content (SPAD) and photosynthesis rate were unaffected by variation of substrate composition. Furthermore, rootstock grown in C1 container showed plant height, number of leaves, leaf area, number of nodes, root length, shoot and root’s fresh and dry weight were significantly higher than those grown in C2 and C3 volume containers. The chlorophyll content and transpiration rate were not significantly affected by the different container volumes. These results suggest that the substrate ratio 3:1:2 of PM:VL:PL and container size 1000 mL were more favorable than other treatments for initial growth and development of the tissue culture propagated apple rootstock plants.

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Responses of Micropropagated Rhubarb (Rheum rhaponticum) Plantlets to Different Growing Media and Light Conditions in the Greenhouse

et al. 2023

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Cultivating red-stalked rhubarb plants is an important source of raw materials for producing health-promoting foods. The quality and quantity of rhubarb crops are significantly dependent on planting material. To obtain high-quality planting material for the value selection of the rhubarb ‘Raspberry’, we evaluated the morphological and physiological responses of micropropagated plantlets to different growth substrates and light quality during early growth ex vitro in the greenhouse. The plantlets were grown in high-EC (GM1) and low-EC (GM2) peat substrates under four light-emitting diodes (LED) light treatments as supplementary lighting (SL) in the wintertime: 100% red (R), 100% blue (B), white light [44.4% green (G), 24.4% B, 28.9% R; 2.2% far red (FR)] and R+B+G+FR (49.4/16.3/10.3/23.8%) light. Compared to the control (natural sunlight), applied LED lighting significantly increased all growth parameters, but only in plantlets grown in GM1 substrate. Among LED treatments, R+B+G+FR light had the most stimulative effect on all growth parameters (length of leaf petioles, leaf area, biomass) and soluble sugar production. Still, it decreased the levels of phenolic compounds in the leaf petioles. Phenolic synthesis, mainly anthocyanins, was the highest under white light (622.8 mg·100 g−1 dry mass), followed by red (601.8 mg·100 g−1), blue (464.4 mg·100 g−1), and R+B+G+FR light (416.4 mg·100 g−1). High anthocyanin accumulation under R-LED light was associated with high antioxidant activity and growth cessation. Hence, for optimal effects related to plant growth and anthocyanin biosynthesis, the use of W-LED lighting is recommended for the early growth ex vitro of micropropagated rhubarb plantlets.

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Growth and Acclimation of In Vitro-Propagated M9 Apple Rootstock Plantlets under Various Visible Light Spectrums

Cited by 4 publications

References 55 publications

Light Quality-Mediated Influence of Morphogenesis in Micropropagated Horticultural Crops: A Comprehensive Overview

Light Quality-Mediated Influence of Morphogenesis in Micropropagated Horticultural Crops: A Comprehensive Overview

Influence of Substrate Composition and Container Size on the Growth of Tissue Culture Propagated Apple Rootstock Plants

Responses of Micropropagated Rhubarb (Rheum rhaponticum) Plantlets to Different Growing Media and Light Conditions in the Greenhouse

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