Growth, anatomy and histochemistry of fast growing species under in vitro conservation through mineral oil and low-temperature combination

Lacerda, Luciana Florêncio de; Gomes, Hugo Teixeira; Bartos, Patrícia Monah Cunha; Vasconcelos, Jaqueline Martins; Filho, Sebastião Carvalho Vasconcelos; Silva‐Cardoso, Inaê Mariê de Araújo; Scherwinski‐Pereira, Jonny Everson

doi:10.1007/s11240-020-01821-6

Cited by 7 publications

(3 citation statements)

References 37 publications

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“…It was emphasized that, compared to osmotic stress, storing in cold and dark conditions facilitated the medium-term conservation of globe artichoke [27]. According to De Lacerda et al (2021) [17], rapidly growing plants P. glomerata and L. filifolia were maintained for up to 360 days with 100% or 50% survival in 5 mL of mineral oil at a temperature of 15 • C, while higher temperatures (20 • C and 25 • C) decreased plant survival. Light is essential for plant growth and development as well as improving cutting survival and regrowth of both ZHB and G-2 in the present study.…”

Section: Discussionmentioning

confidence: 99%

“…Slow growth of plant germplasm via medium-term conservation based on in vitro micropropagation reduces costs [16]. This method is usually conducted by reducing the culture temperature, supplying osmotic agents in culture medium, and adding growth inhibitors to or removing growth promoters from the medium [17,18]. To date, several plant germplasms have been successfully conserved using this method.…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Techniques for Shipping of Micropropagated Plant Materials

et al. 2022

Horticulturae

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Shipping of in vitro micro-cuttings in tubes or jars is a frequently used method as the plants are more likely to quickly reproduce and comply with quarantine regulations in plant germplasm distribution. However, these containers are fragile during transportation. To diminish the risk associated with the long-distance shipping of in vitro plants, a safe and widely applicable packing and conservation technique based on microplate and slow growth was developed in this study. Potato cultivar ZHB and ginger cultivar G-2 were used to optimize the system with microplates (96 wells), vacuum-sealed packaging, and slow-growth techniques. Under regular culture conditions, packing in vacuum-sealed microplates reduced the survival of ZHB and G-2 micro-cuttings to 85.8% and 20.0%, respectively, and regeneration to 61.8% and 0%, respectively. Reducing the temperature to 10 °C maintained the survival of ZHB and G-2 micro-cuttings in the range of 83.3–100% after 60 days. Exposure to darkness decreased the survival of G-2 and inhibited regrowth. Thus, conservation in darkness at 10 °C is suggested. The effects of iron concentration and plant growth retardants were further assessed. The addition of 1/4 MS medium combined with 100 mg/L chlormequat chloride (CCC) resulted in full survival and growth inhibition of plantlets, without malformation identified. Finally, incubation with 1/4 MS medium supplemented with 100 mg/L CCC in vacuum-sealed microplates at 10 °C in the dark resulted in high survival and suppressed germination. Sweet potato HXS was incubated as well to test the broad-spectrum applications of the technique; 100% survival and 6.7% germination was gained. Morphological indices of released cuttings recovered to control levels after two cycles of subculture in MS medium. A 0.1–0.2% genetic variation was detected by SSR and ISSR, suggesting genetic stability of the conserved samples. Finally, micro-cuttings were safely transported to cities located thousands of kilometers away without package and sample damage. Our results enable easy distribution of in vitro plant germplasms.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In Vitro Techniques for Shipping of Micropropagated Plant Materials

et al. 2022

Horticulturae

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show abstract

“…In a similarly compelling review, Bettoni et al (2021) examined the challenges that still require optimization for successful implementation of the cryopreservation procedures to shoot tips. The difficulties for technology transfer between laboratories, which has often slowed down and sometimes even impeded protocol validation, were discussed and most critical factors involved were clearly identified.…”

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confidence: 99%