Growth ability in various macaque cell lines of HIV-1 with simian cell-tropism

Natural Single-Nucleotide Polymorphisms in the 3′ Region of the HIV-1 pol Gene Modulate Viral Replication Ability

“…Virion-associated reverse transcriptase (RT) activity was measured as previously described (37). M8166 and MT4/CCR5 cells (10 5 ) were infected with equal amounts of NL-DT5R and its derivative viruses (35 RT units and 5 ϫ 10 5 RT units for M8166 and MT4/CCR5 cells, respectively), as previously described (38,39). Equal amounts (10 5 RT units) of HIV-1 NL4-3 and its derivative viruses were inoculated into MT4/CCR5 cells (10 5 ).…”

Growth potentials of CCR5-tropic/CXCR4-tropic HIV-1mt clones in macaque cells

“…We selected three distinct Env sequences and made three proviral constructs in the backbone of the NL-DT5R genome to obtain CCR5-tropic/dual-tropic viruses (Figure 1A), based on the published results (Hsu et al, 2003; Hatziioannou et al, 2006; Matsuda et al, 2010; Nishimura et al, 2010). Of the three clones constructed, while NL-DT562 grew in a cynomolgus macaeque cell line HSC-F (Akari et al, 1996; Fujita et al, 2003), the other two viruses designated NL-DT589 and NL-DT5AD did not (Doi et al, 2010; our unpublished observations). The replication efficiency in HSC-F cells of NL-DT562 was much lower than that of the parental virus NL-DT5R (Doi et al, 2010).…”

“…Of the three clones constructed, while NL-DT562 grew in a cynomolgus macaeque cell line HSC-F (Akari et al, 1996; Fujita et al, 2003), the other two viruses designated NL-DT589 and NL-DT5AD did not (Doi et al, 2010; our unpublished observations). The replication efficiency in HSC-F cells of NL-DT562 was much lower than that of the parental virus NL-DT5R (Doi et al, 2010). When examined in CD8 + cell-depleted pig-tailed macaque peripheral blood mononuclear cells (PBMCs), NL-DT5AD was found to be replication-competent in addition to NL-DT562 (Igarashi and Adachi, unpublished results).…”

Macaque-Tropic HIV-1 Derivatives: A Novel Experimental Approach to Understand Viral Replication and Evolution in Vivo

“…Cell lines for virological evaluation of HIV-1mt. TRIM5 alleles of the cell lines listed have been determined in our laboratory (Doi et al, 2010;our unpublished results). For the polymorphism of TRIM5 alleles, see the references (Newman et al, 2006;Virgen et al, 2008;Wilson et al, 2008).…”

“…In relatively resistant M1.3S cells, three clones do replicate but the others do not. In both cell lines, SIVmac239 (MA239N) (Doi et al, 2010) displays the best potential to replicate. These results indicate that we still need to improve MN4Rh-3V and MN5Rh-3V to obtain the ideal clone, the pathogenic HIV-1mt.…”