Growing Magnetotactic Bacteria of the Genus &lt;em&gt;Magnetospirillum&lt;/em&gt;: Strains MSR-1, AMB-1 and MS-1

Nagard, Lucas Le; Morillo-López, Viviana; Fradin, Cécile; Bazylinski, Dennis A.

doi:10.3791/58536-v

Cited by 4 publications

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“…The MTB strain AMB‐1 is typically cultured between 25 and 30 °C in iron‐rich media under microaerophilic conditions. [ 22 ] Calugay et al. found that MTB proliferation increased and magnetosome formation decreased in low iron media, [ 23 ] such as DMEM.…”

Section: Resultsmentioning

confidence: 99%

“…The MTB strain AMB-1 is typically cultured between 25 and 30 °C in iron-rich media under microaerophilic conditions. [22] Calugay et al found that MTB proliferation increased and magnetosome formation decreased in low iron media, [23] such as DMEM. Since magnetic cells synthesize highly organized magnetosomes and have a higher metabolic burden, their growth rate is slower than that of nonmagnetic cells.…”

Section: Mtb Proliferation Magnetic Responsiveness and Viability Unde...mentioning

confidence: 99%

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Engineering Living Immunotherapeutic Agents for Improved Cancer Treatment

Gwisai,

Günther,

Mirkhani

et al. 2023

Advanced Therapeutics

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Bacteria‐based agents are emerging as promising tools for cancer therapy due to their ability to actively target tumors, trigger localized inflammation, and induce tumor regression. There has been growing interest in using bacteria that are responsive to external cues, such as magnetic fields, to facilitate the formation of robust colonies in tumors and achieve the threshold for clinical efficacy. Several studies have demonstrated the potential of innately magnetically responsive bacteria, known as magnetotactic bacteria (MTB), as steerable agents. However, their immunostimulatory properties, which play a central role in their function as therapeutic agents, have not yet been adequately studied. Here, key aspects of human immune cell response to MTB strain Magnetospirillum magneticum AMB‐1 in physiological environments are characterized. The ability of MTB to maintain magnetic properties, remain viable in whole blood, elicit cytokine production by macrophages, and stimulate uptake of cancer cell material by dendritic cells is examined. This study also investigates the use of MTB–liposome complexes for effective delivery of therapeutic payloads in vitro and explores response to the agent in vivo. Overall, this work establishes the potential of MTB as a versatile, combined delivery platform for immune‐mediated cancer therapy.

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Section: Resultsmentioning

confidence: 99%

Section: Mtb Proliferation Magnetic Responsiveness and Viability Unde...mentioning

confidence: 99%

Engineering Living Immunotherapeutic Agents for Improved Cancer Treatment

Gwisai,

Günther,

Mirkhani

et al. 2023

Advanced Therapeutics

View full text Add to dashboard Cite

show abstract

“…These findings suggest that while MTB have higher rates of proliferation at 37 °C in DMEM, biomineralization is impaired at this temperature. The MTB strain AMB-1 is typically cultured between 25 ˚C and 30 ˚C in iron rich media under microaerophilic conditions (18). Calugay et al found that MTB proliferation increased and magnetosome formation decreased in low iron media (19), such as DMEM.…”

Section: Mtb Proliferation Under Physiological Conditions and Clearan...mentioning

confidence: 99%

Engineering living immunotherapeutic agents for improved cancer treatment

Gwisai

Günther

Vizovišek

et al. 2023

Preprint

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Bacteria-based biohybrid agents are emerging as a promising strategy for cancer therapy due to their ability to actively target tumors, trigger localized inflammation and induce tumor regression. There has been growing interest in using bacteria that are responsive to external cues, such as magnetic fields, to facilitate the formation of robust colonies in tumor the achieve the threshold for clinical efficacy. Several studied have demonstrated the potential of innately magnetically responsive bacteria, known as magnetotactic bacteria (MTB), as steerable agents, however, their immunostimulatory properties and therapeutic effects are yet to be explored. Here, we characterize key properties of human immune cell responses and the behavior of the MTB strain Magnetospirillum magneticum AMB-1 in physiological environments. This work investigates the ability of MTB to maintain magnetic properties, viability in whole blood, cytokine production by macrophages, and the ability to increase uptake of cancer cell material by dendritic cells. This study also explores the use of MTB-liposome complexes for effective delivery of therapeutic payloads. Overall, this study establishes the potential of MTB as a versatile, combined drug delivery platform for immune-mediated cancer therapy.

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“…Amongst them, some cultivable species have been bred as type strains and used for stress exposure experiments. Different strains are sensitive to different stress factors (Table 1 ), nutrients, and dissolved oxygen levels [ 34 , 35 ].…”

Section: Stress Tolerance Of Mtbmentioning

confidence: 99%

Renaissance for magnetotactic bacteria in astrobiology

Shen,

Paterson,

Wang

et al. 2023

The ISME Journal

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Capable of forming magnetofossils similar to some magnetite nanocrystals observed in the Martian meteorite ALH84001, magnetotactic bacteria (MTB) once occupied a special position in the field of astrobiology during the 1990s and 2000s. This flourish of interest in putative Martian magnetofossils faded from all but the experts studying magnetosome formation, based on claims that abiotic processes could produce magnetosome-like magnetite crystals. Recently, the rapid growth in our knowledge of the extreme environments in which MTB thrive and their phylogenic heritage, leads us to advocate for a renaissance of MTB in astrobiology. In recent decades, magnetotactic members have been discovered alive in natural extreme environments with wide ranges of salinity (up to 90 g L−1), pH (1–10), and temperature (0–70 °C). Additionally, some MTB populations are found to be able to survive irradiated, desiccated, metal-rich, hypomagnetic, or microgravity conditions, and are capable of utilizing simple inorganic compounds such as sulfate and nitrate. Moreover, MTB likely emerged quite early in Earth’s history, coinciding with a period when the Martian surface was covered with liquid water as well as a strong magnetic field. MTB are commonly discovered in suboxic or oxic-anoxic interfaces in aquatic environments or sediments similar to ancient crater lakes on Mars, such as Gale crater and Jezero crater. Taken together, MTB can be exemplary model microorganisms in astrobiology research, and putative ancient Martian life, if it ever occurred, could plausibly have included magnetotactic microorganisms. Furthermore, we summarize multiple typical biosignatures that can be applied for the detection of ancient MTB on Earth and extraterrestrial MTB-like life. We suggest transporting MTB to space stations and simulation chambers to further investigate their tolerance potential and distinctive biosignatures to aid in understanding the evolutionary history of MTB and the potential of magnetofossils as an extraterrestrial biomarker.

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Growing Magnetotactic Bacteria of the Genus <em>Magnetospirillum</em>: Strains MSR-1, AMB-1 and MS-1

Cited by 4 publications

References 0 publications

Engineering Living Immunotherapeutic Agents for Improved Cancer Treatment

Engineering Living Immunotherapeutic Agents for Improved Cancer Treatment

Engineering living immunotherapeutic agents for improved cancer treatment

Renaissance for magnetotactic bacteria in astrobiology

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