Grouping of some clinically relevant Gram‐positive rods by automated fatty acid analysis

Graevenitz, Alexander von; Osterhout, Gerard; Dick, James D.

doi:10.1111/j.1699-0463.1991.tb05132.x

Cited by 51 publications

(50 citation statements)

References 21 publications

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“…Tuberculostearic acid is relatively rarely detected in non-lipophilic fermentative corynebacteria. In fact, Corynebacterium rninutissimum and Corynehacfitevium xerosis are the only two non-lipophilic, fermentative corynebacteria in which tuberculostearic acid is detected (Bernard et al, 1991 ;von Graevenitz et al, 1991).…”

Section: Resultsmentioning

confidence: 99%

Corynebacterium confusum sp. nov., isolated from human clinical specimens

Funke

Osorio²,

Frei³

et al. 1998

International Journal of Systematic Bacteriology

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Three strains of a previously unknown coryneform bacterium were isolated from two patients with foot infections and from a blood culture of a third patient. The three non-lipophilic strains exhibited very slow fermentative acid production from glucose but not from maltose or sucrose, nitrate reductase activity, no tyrosinase activity and the presence of small amounts of tuberculostearic acid as the most significant phenotypic features. Differentiation of these strains from all other presently defined coryneform bacteria was readily achieved. Chemotaxonomic investigations revealed that the three strains unambiguously belonged to the genus Corynebacterium. Comparative 165 rRNA gene sequence analysis demonstrated that the isolates were almost identical and represented a new subline within the genus Corynebacterium, for which the designation Corynebacterium confusum sp. nov. is proposed. The type strain of Corynebacterium confusum is CCUG 38267T.

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Section: Resultsmentioning

confidence: 99%

Corynebacterium confusum sp. nov., isolated from human clinical specimens

Funke

Osorio²,

Frei³

et al. 1998

International Journal of Systematic Bacteriology

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“…Before analysis, isolates were sub-cultured on 4% (w/v) Trypticase Soy Broth agar and incubated at 28°C for 24 hours to ensure a typical FAME profile. Similarity indices of ≥ 0.500 (excellent species-level match between sample and reference isolates: [63]) were used as the demarcation for positive identification. Where two or more possible matches were given, the closest match was accepted provided that the similarity index separation between this and the second match was ≥ 0.200 (double the minimum recommended separation: [30]).…”

Section: Microbial Identificationmentioning

confidence: 99%

Intraspecific Variation and Interspecific Differences in the Bacterial and Fungal Assemblages of Blue Tit (Cyanistes caeruleus) and Great Tit (Parus major) Nests

Goodenough

Stallwood

2009

Microb Ecol

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“…The methods used for analyses of cellular fatty acids (gas-liquid chromatography [25]), cell wall diamino acids (thin-layer chromatography [S]), mycolic acids (thin-layer chromatography [S]), the acyl type of the muramic acid (tube test [23]), and the G + C content (high-performance liquid chromatography [7]) have been described previously.…”

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confidence: 99%

Corynebacterium mucifaciens sp. nov., an Unusual Species from Human Clinical Material

Funke

Lawson

Collins

1997

International Journal of Systematic Bacteriology

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Eight strains of a previously undescribed coryneform bacterium had been isolated from human clinical material over a 5-year period. Colonies of the unknown coryneform bacterium had an unusual appearance as they were slightly yellowish and very mucoid. Biochemical and chemotaxonomic characterization revealed that the unknown coryneform bacterium belonged to the genus Corynebacterium. It could be readily differentiated from all previously described Corynebacterium species. Electron microscopy demonstrated the production of an extracellular substance causing connecting filaments between cells as a morphological correlate to the mucoid colonies. Comparative 16s rRNA gene sequence analysis revealed that the unknown coryneform bacterium represented a new subline within the genus Corynebacterium, for which the name Corynebacteriurn mucifaciens sp. nov. is proposed. The type strain is CCUG 36878 (= DSM 44265 = CIP 105129).The genus Corynebacterium is the largest genus in the coryneform group of bacteria (i.e., aerobically growing, asporogenous, non-partially acid-fast, irregular gram-positive rods) and currently comprises more than 25 species (1 1). During the past decade, 12 new Corynebacterium species have been established (9,ll). Interestingly, all of these were derived from human clinical specimens, whereas only 7 of the 17 Corynebacterium species listed in Bergey's Manual of Systematic Bacteriology are considered medically relevant (4, 11). The present report deals with yet another novel Corynebacterium species isolated from human clinical specimens. Over a 5-year period, eight strains of a coryneform bacterium whose colonies were slightly yellowish and markedly mucoid were isolated from human clinical material. In a polyphasic approach including both phenotypic and molecular genetic methods, it was demonstrated that this coryneform bacterium represents a new Corynebacterium species, for which the name Corynebacterium rnucifaciens sp. nov. is proposed. MATERIALS AND METHODSBacterial strains. The origins of the strains studied and their clinical sources are given in Table 1. Four strains were isolated by workers at the Department of Medical Microbiology, University of Zurich, Zurich, Switzerland, and four other isolates were received by the reference laboratory for coryneform bacteria of the same institution.Phenotypic characteristics. Strains were grown aerobically at 37°C in a 5% C0,-enriched atmosphere on Columbia agar (Difco, Detroit, Mich.) supplemented with 5% sheep blood. The methods used for biochemical profiling of the bacteria have been outlined in detail previously (8). By using the API Coryne system (bioMCrieux, Marcy l'Etoile, France), enzymatic reactions were determined after 24 h at 3TC, and acid production from carbohydrates was observed after 48 h of incubation. The API S0CH system (acid production from additional carbohydrates) was used in conjunction with 50CHE medium (both from bioMerieux), and reactions were read after 96 h of incubation at 37°C.Electron microscopy. Cells were grown in brain hea...

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Grouping of some clinically relevant Gram‐positive rods by automated fatty acid analysis

Cited by 51 publications

References 21 publications

Corynebacterium confusum sp. nov., isolated from human clinical specimens

Corynebacterium confusum sp. nov., isolated from human clinical specimens

Intraspecific Variation and Interspecific Differences in the Bacterial and Fungal Assemblages of Blue Tit (Cyanistes caeruleus) and Great Tit (Parus major) Nests

Corynebacterium mucifaciens sp. nov., an Unusual Species from Human Clinical Material

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