Grouping of Porcine Enteroviruses by Indirect Immunofluorescence and Description of Two New Serotypes

Auerbach, Joachim; Prager, D.; Neuhaus, Stefan; Loss, U.; Witte, K. H.

doi:10.1111/j.1439-0450.1994.tb00228.x

Cited by 35 publications

(28 citation statements)

References 9 publications

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“…Extents of pairwise amino acid identity range from 79 to 87% for 1AB, 76 to 91% for 1C, and 66 to 82% for 1D. Phylogenetic tree construction using the Puzzle program supports the existence of distinct serotypes, which were demonstrated with serological methods (3,23) (Tables 2 and 3). For this analysis, representative members of the most closely related genera cardiovirus, aphthovirus, and erbovirus (ERBV) were included to illustrate both the genetic distance of the PTV serotypes to the heterologous genera and the close relationship within the teschoviruses (Fig.…”

Section: Resultsmentioning

confidence: 95%

“…Of the 13 previously described serotypes of PEV, 10 were subclassified into CPE group I (3,23). Molecular data describing their relation to PTV-1 strain F65 are missing.…”

Section: Resultsmentioning

confidence: 99%

“…These characteristics in combination with the growth properties in different host cell lines and the cell type specificity were the basis of a subclassification into three CPE groups (23,42). Ten acknowledged serotypes comprise CPE group I, the main group (3,23). However, significant cross-reaction of serotypespecific antibodies led sometimes to ambiguous serotyping.…”

Section: Discussionmentioning

confidence: 99%

“…Due to the physicochemical properties of their virions, they were previously classified as enteroviruses. Several distinct serotypes have been described (3,5,6,12,17,23,36). Based on parameters such as (i) cytopathic effect (CPE), (ii) replication properties in various host cell lines, and (iii) serological assays, the PEVs were divided into three CPE groups: I (serotypes 1 to 7 and 11 to 13), II (serotype 8), and III (serotypes 9 and 10) (23, 42).…”

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confidence: 99%

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Porcine Teschoviruses Comprise at Least Eleven Distinct Serotypes: Molecular and Evolutionary Aspects

Zell¹,

Dauber

Krumbholz³

et al. 2001

J Virol

108

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Nucleotide sequencing and phylogenetic analysis of 10 recognized prototype strains of the porcine enterovirus (PEV) cytopathic effect (CPE) group I reveals a close relationship of the viral genomes to the previously sequenced strain F65, supporting the concept of a reclassification of this virus group into a new picornavirus genus. Also, nucleotide sequences of the polyprotein-encoding genome region or the P1 region of 28 historic strains and recent field isolates were determined. The data suggest that several closely related but antigenically and molecular distinct serotypes constitute one species within the proposed genus Teschovirus. Based on sequence data and serological data, we propose a new serotype with strain Dresden as prototype. This hitherto unrecognized serotype is closely related to porcine teschovirus 1 (PTV-1, former PEV-1), but induces typespecific neutralizing antibodies. Sequencing of field isolates collected from animals presenting with neurological disorders prove that other serotypes than PTV-1 may also cause polioencephalomyelitis of swine.The porcine enteroviruses (PEVs) were described as causative agents of severe and mild neurological disorders known as Teschen/Talfan disease (16, 39), reproductive failure (10), pneumonia (27), diarrhea (13), and dermal lesions of swine (25). Due to the physicochemical properties of their virions, they were previously classified as enteroviruses. Several distinct serotypes have been described (3,5,6,12,17,23,36). Based on parameters such as (i) cytopathic effect (CPE), (ii) replication properties in various host cell lines, and (iii) serological assays, the PEVs were divided into three CPE groups: I (serotypes 1 to 7 and 11 to 13), II (serotype 8), and III (serotypes 9 and 10) (23, 42). Recently, partial genome regions of members of each CPE group-Porcine teschovirus 1 (PTV-1) strains Talfan and F65 (9,20), PEV-8 (J. H. Peng, R. P. Kitching, and N. J. Knowles, unpublished data), and PEV-9 (J. H. Peng, J. W. McCauley, R. P. Kitching, and N. J. Knowles, unpublished data)-were cloned and sequenced. Analysis of available genome information revealed that PEV-9 and PEV-10 are typical enteroviruses, while PEV-8 appears to be closely related to both enteroviruses and rhinoviruses. However, the genome of strain F65 (a member of CPE group I) exhibits unique features suggesting the reclassification into a new picornavirus taxon. The new genus was named Teschovirus, with F65 as a member of the species Porcine teschovirus (22). In this study, we will use the proposed name "teschovirus" as synonymous to "PEV CPE group I."Since the molecular properties of other members of PEV CPE group I and their relationship to F65 are still unclear, we aimed to gain further information on these viruses. For this purpose, the genomes of the prototype strains of PEV-1 to -7 and PEV-11 to -13 (proposed names PTV-1 to -10 [ Table 1]) were sequenced and compared to genomes of other members of the family Picornaviridae. Moreover, several historic strains and recent field isolates were i...

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Section: Resultsmentioning

confidence: 95%

“…Of the 13 previously described serotypes of PEV, 10 were subclassified into CPE group I (3,23). Molecular data describing their relation to PTV-1 strain F65 are missing.…”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Porcine Teschoviruses Comprise at Least Eleven Distinct Serotypes: Molecular and Evolutionary Aspects

Zell¹,

Dauber

Krumbholz³

et al. 2001

J Virol

108

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“…Today, PTV-1 is still frequently isolated from the faeces, tonsils, and other non-neural organs of clinically unaffected pigs. Moreover, other serotypes than PTV-1 are increasingly identified to be the cause of neurological disorders in swine (Auerbach et al 1994). Determination of whether this observation reflects changes of virus prevalence or is the result of improved methods of virus detection (Zell et al 2000) and virus isolation awaits further investigations (Zell et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence of Antibodies against Porcine Teschovirus 1 in Lithuania

Sereika¹,

Lelešius²,

Zienius³

2007

Acta Vet. Brno

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The objective of the present work was to study the serological status and epidemiological situation of porcine teschovirus 1 (PTV-1) on swine farms in different Lithuanian regions by using virus neutralization test and virus isolation.Clinical, epidemiological, serological and virological examinations were performed for the diagnosis of Teschen disease (porcine enterovirus encephalomyelitis, TE). Swine blood serum samples (n = 1680) were collected on 28 farms (60 samples per farm) of 8 regions in 2003. For virus isolation 29 samples of pathological material were taken from the brains of pigs (2 -4 months of age) that died suddenly on seven swine farms in five regions.Epidemiological and clinical examination revealed no signs of porcine enterovirus encephalomyelitis on 32 Lithuanian swine farms. PTV-1 was not isolated and identified in the PK-15 cell culture. Antibodies against PTV-1, detected by using VNT, were found on all investigated farms and in all age groups. Negative serum (2.3%, 39 from 1680) samples were found in 15.5% (16 from 103) of 2-4-month-old pigs and in 4.7% (23 from 494) of 4-6-month-old ones. The positive serological and negative epidemiological, clinical and virological results suggest that less virulent or avirulent PTV-1 strains were spread on Lithuanian swine farms. Teschen disease, porcine enterovirus encephalomyelitis

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Teschovirus

Zell¹,

Hoey²

2011

The Springer Index of Viruses

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Grouping of Porcine Enteroviruses by Indirect Immunofluorescence and Description of Two New Serotypes

Cited by 35 publications

References 9 publications

Porcine Teschoviruses Comprise at Least Eleven Distinct Serotypes: Molecular and Evolutionary Aspects

Porcine Teschoviruses Comprise at Least Eleven Distinct Serotypes: Molecular and Evolutionary Aspects

Seroprevalence of Antibodies against Porcine Teschovirus 1 in Lithuania

Teschovirus

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