Grouping and genetic diversity of different watermelon ecotypes based on agro-morphological traits and ISSR marker

Abstract

ABSTRACT-The genetic diversity of 38 ecotypes of watermelon was evaluated using genetic markers and morphological traits. Analysis of variance showed a signifi cant diff erence for the studied traits. Cluster analysis using UPGMA method based on morphological traits was classifi ed studied ecotypes into four groups. Using 11 primers were obtained 89 polymorphic bands that cluster analysis of molecular data was placed 38 ecotypes into four groups. The genetic diversity structure of the watermelon accessions on the basis of ISSR data evidenced a common pattern of molecular markers. The similarity of ecotypes grouping with molecular markers and morphological traits was low and the correlation coeffi cient of the twomatrices was low (r = 0.03), revealing quite a non-signifi cant correlation between them and the effi ciency of them to assays in estimating genetic diversity in watermelon is diff erent. Overall, this study demonstrated high genetic diversity among cultivated watermelon which may be attributed to their high genetic background and environmental eff ects. Keywords: genetic diversity, ISSR, marker RESUMO -Agrupamento e diversidade genética de diferentes ecótipos de melancia com base em características agro-morfológicas e ISSR.A diversidade genética de 38 ecótipos de melancia foi avaliada usando marcadores genéticos e características morfológicas. A análise de variância mostrou diferença signifi cativa para os caracteres estudados. A análise de agrupamento usando o método UPGMA baseado em características morfológicas foi classifi cada os ecótipos em quatro grupos. Utilizando 11 primers foram obtidas 89 bandas polimórfi cas que agruparam a análise de dados moleculares em 38 ecótipos em quatro grupos. A estrutura de diversidade genética dos acessos de melancia com base nos dados do ISSR evidenciou um padrão comum de marcadores moleculares. A semelhança do agrupamento de ecótipos com marcadores moleculares e características morfológicas foi baixa e o coefi ciente de correlação das duas matrizes foi baixo (r = 0,03), revelando uma correlação bastante signifi cativa entre eles e a efi ciência dos mesmos em estimar a diversidade genética em melancia é diferente. No geral, este estudo demonstrou alta diversidade genética entre a melancia cultivada, o que pode ser atribuído ao seu elevado background genético e efeitos ambientais. Palavras-chave: diversidade genética, ISSR, marcador

Genetic diversity analysis reveals weak population structure in invasive Trianthema portulacastrum L. at Fayoum depression, Egypt

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Investigate Genetic Relation Among Watermelon Cultivars Using Molecular Dna Markers

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Genetic diversity and population structure of watermelon (Citrullus sp.) genotypes

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