Group II phospholipase A2 in serum in critically ill surgical patients

Jm, Grönroos; Kuttila, Kari; Tj, Nevalainen

doi:10.1097/00003246-199406000-00013

Cited by 24 publications

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“…Serum PLA levels have been shown to correlate with the mortality of intensive care patients. 343 In 67 consecutive patients with multiple injuries, 44 patients with peritonitis, and 51 patients with sepsis admitted to a surgical intensive care unit catalytic activity and mass concentration of PLA and CRP in blood were measured daily during each patient's stay in the intensive care unit. PLA 2 -II mass concentration correlated significantly with the catalytic activity of PLA 2 and CRP values.…”

Section: F Pla 2 In Multiple Organ Failure (Mof)mentioning

confidence: 99%

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Phospholipase A₂: Its Usefulness in Laboratory Diagnostics

Kaiser

1999

Critical Reviews in Clinical Laboratory Sciences

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Phospholipase A2 (PLA2) is an enzyme that catalyzes the hydrolysis of membrane phospholipids. This article reviews the source and structure of PLA2, the involvement of the enzyme in various biological and pathological phenomena, and the usefulness of PLA2 assays in laboratory diagnostics. Of particular importance is the role of PLA2 in the cellular production of mediators of inflammatory response to various stimuli. Assays for PLA2 activity and mass concentration are discussed, and the results of enzyme determinations in plasma from patients with different pathological conditions are presented. The determination of activity and mass concentration in plasma is particularly useful in the diagnosis and prognosis of pancreatitis, multiple organ failure, septic shock, and rheumatoid arthritis. A very important result is the demonstration that PLA2 is an acute phase protein, like CRP. Indeed, there is a close correlation between PLA2 mass concentration and CRP levels in several pathological conditions. Although the determination of C-reactive protein is much easier to perform and is routinely carried out in most clinical laboratories, the assessment of PLA2 activity or mass concentration has to be considered as a reliable approach to obtain a deeper understanding of some pathological conditions and may offer additional information concerning the prognosis of several disorders.

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Section: F Pla 2 In Multiple Organ Failure (Mof)mentioning

confidence: 99%

“…Severe infections, disorders involving tissue destruction, and elective operations per se, caused considerable increases in PLA 2 -II mass concentrations. 343 Results are illustrated in Table 5.…”

Section: F Pla 2 In Multiple Organ Failure (Mof)mentioning

confidence: 99%

Phospholipase A₂: Its Usefulness in Laboratory Diagnostics

Kaiser

1999

Critical Reviews in Clinical Laboratory Sciences

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“…The best characterized function of group I phospholipase A 2 is digestion of the phospholipid component of dietary fat (14), but the enzyme also functions as a growth factor (1) and vasoactive substance (15), and stimulates prostaglandin synthesis (16). The amount of group II phospholipase A 2 is markedly increased in serum during infection and inflammation (17,18), and the enzyme may function in defence against bacteria (19). The amount of group II phospholipase A 2 is markedly increased in serum during infection and inflammation (17,18), and the enzyme may function in defence against bacteria (19).…”

Section: Introductionmentioning

confidence: 99%

Serum Phospholipases A₂ after Aortobifemoral Reconstruction

Grönroos¹,

Kuttila²,

Perttilä³

et al. 1995

CCLM

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Phospholipase A 2 has been implicated in the pathogenesis of local and distant tissue injury after ischaemia and reperfusion. A common operation inducing ischaemia and reperfusion is aortobifemoral reconstruction, during which the aorta is clamped and the blood supply via the inferior mesenteric artery and iliac arteries is interrupted. The purpose of the present work was to study the catalytic activity concentration of phospholipase A 2 and the mass concentrations of group I and group II phospholipases A 2 in the sera of patients undergoing aortobifemoral reconstruction. Both the catalytic activity concentration of phospholipase A 2 and the mass concentrations of group I and group II phospholipases A 2 increased in serum samples after the operation. The catalytic activity concentration of phospholipase A 2 correlated well with group II phospholipase A 2 mass values (r = 0.81, p < 0.001), whereas no correlation was found between the catalytic activity concentration of phospholipase A 2 and group I phospholipase A 2 mass values (r = 0.12, p = 0.54). The results emphasize the role of group II phospholipase A 2 in tissue injury after ischaemia and reperfusion.

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“…This enzyme is present in numerous human tissues and body fluids (11). The concentration of group II PLA2 in serum increases during severe acute inflammatory diseases (20) and after surgery (7,17). The catalytic activity of PLA2 found in blood plasma is due to the presence of group II PLA2 during various pathological conditions, including sepsis (6), bacterial infections (29), arthritis (14), acute pancreatitis (21), and multiple organ failure (25).…”

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Resistance of Transgenic Mice Expressing Human Group II Phospholipase A2 toEscherichia coliInfection

Laine

Grass²,

Nevalainen

2000

Infect Immun

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؉ mice and their PLA2-deficient C57BL/6J littermates (PLA2 ؊ mice) were studied after intraperitoneal administration of E. coli. The levels of group II PLA2 in sera of PLA2 ؉ mice increased after the administration of E. coli, and the concentration of group II PLA2 correlated significantly with the catalytic activity of PLA2 in serum. PLA2؉ mice showed lower rates of mortality and less bacterial growth in peritoneal lavage fluid, blood, and spleen and liver tissues than PLA2 ؊ mice. Unlike the observations with staphylococcal infection, serum and peritoneal lavage fluid did not inhibit the growth of E. coli in vitro. The results indicate that expression of the group II PLA2 transgene improves the host defense of mice against E. coli infection.

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Group II phospholipase A2 in serum in critically ill surgical patients

Cited by 24 publications

References 0 publications

Phospholipase A₂: Its Usefulness in Laboratory Diagnostics

Phospholipase A₂: Its Usefulness in Laboratory Diagnostics

Serum Phospholipases A₂ after Aortobifemoral Reconstruction

Resistance of Transgenic Mice Expressing Human Group II Phospholipase A2 toEscherichia coliInfection

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Group II phospholipase A2 in serum in critically ill surgical patients

Cited by 24 publications

References 0 publications

Phospholipase A2: Its Usefulness in Laboratory Diagnostics

Phospholipase A2: Its Usefulness in Laboratory Diagnostics

Serum Phospholipases A2 after Aortobifemoral Reconstruction

Resistance of Transgenic Mice Expressing Human Group II Phospholipase A2 toEscherichia coliInfection

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Product

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Phospholipase A₂: Its Usefulness in Laboratory Diagnostics

Phospholipase A₂: Its Usefulness in Laboratory Diagnostics

Serum Phospholipases A₂ after Aortobifemoral Reconstruction