Group A Streptococcal
            <i>rofA</i>
            Gene Is Involved in the Control of Several Virulence Genes and Eukaryotic Cell Attachment and Internalization

Beckert, Susanne; Kreikemeyer, Bernd; Podbielski, Andreas

doi:10.1128/iai.69.1.534-537.2001

Cited by 69 publications

(70 citation statements)

References 36 publications

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“…The corresponding nra49 gene sequence (accession number U49397 ; Podbielski et al, 1999) exhibited only 62n6% sequence homology. The rofA2 gene was inactivated by insertion mutagenesis (Beckert et al, 2001) and the mutation was confirmed by PCR and Southern hybridization (data not shown). No differences in the growth rate and final optical cell density between wild-type and mutant was detected (data not shown).…”

Section: Construction Of a Rofa Mutant In The Gas Serotype M2mentioning

confidence: 92%

“…All mutant strains were maintained on medium supplemented with 60 mg spectinomycin l − ". The RofA6 and Nra49 mutant strains have been described elsewhere (Beckert et al, 2001 ;Molinari et al, 2001). The EmmLuc recombinant strain was constructed in this study according to methods published by Podbielski et al (1999).…”

Section: Methodsmentioning

confidence: 99%

“…Construction of the pSF152-rofA6 plasmid used for insertional inactivation of the rofA2 allele was described by Beckert et al (2001). The Qiagen Mini Prep kit was used to prepare plasmid DNA from Escherichia coli.…”

Section: Methodsmentioning

confidence: 99%

“…Determination of haemolytic activity contained in supernatants of wild-type and RofA2 mutant bacteria and measurement of binding capacity towards extracellular matrix and plasma proteins was performed as described previously (Kreikemeyer et al, 2001). Culture and maintenance of the human laryngeal epithelial cell line HEp-2 (ATCC CCL23,) and adherence and invasion assays using wild-type and mutant bacteria were essentially as described by Molinari et al (2001).…”

Section: Methodsmentioning

confidence: 99%

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Group A streptococcal RofA-type global regulators exhibit a strain-specific genomic presence and regulation pattern

et al. 2002

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RofA-like protein (RALP) type regulators have been shown to exist in different forms in group A streptococci (GAS) and to regulate the expression of important bacterial adhesins. This study shows that the vast majority of strains from different GAS M serotypes carried a rofA virulence regulator gene in their genome and that this gene could be detected in combination with other RALP genes and RALP-dependent adhesin genes in a strain-specific manner. The gene encoding the Nra regulator was predominantly found in opacity factor (OF)-negative serotypes. When analysing a rofA mutant in a serotype M2 strain, the strain specificity was also found in the positive and negative regulatory functions of RALP genes as well as in the type and number of virulence genes and functions controlled by the RALP genes. Of 17 virulence-associated genes tested, only one, the putative streptolysin S gene, was observed to be derepressed in RALP mutants of three different GAS serotype strains. This strain-specific variability of RALP regulon sizes is associated with different patterns of host cell attachment and internalization. In addition, RofA2 was shown to control expression of the ribosomal protein gene rpsL. As a consequence, it was demonstrated for the first time in streptococci that aminoglycoside resistance mediated by rpsL expression is apparently controlled by a virulence gene regulator.

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Section: Construction Of a Rofa Mutant In The Gas Serotype M2mentioning

confidence: 92%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Group A streptococcal RofA-type global regulators exhibit a strain-specific genomic presence and regulation pattern

et al. 2002

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“…CodY, Irr, SLS and SptR regulate positively the operon [56][57][58][59] whereas CcpA, CovR, FasX, LuxS, Mga and RopB and members of the Ralp regulator family including RofA, Nra and Ralp3 have repressing effects. 42,56,57,[60][61][62][63][64][65][66][67][68][69][70][71] The nutritional status of the cells also has regulatory roles on sag/pel expression. 46,72 How Pel controls the expression of virulence factors has not yet been deciphered and no primary targets are known.…”

Section: Integration Of Srnas Into the Regulatory Network Of Streptomentioning

confidence: 99%

Small RNAs in streptococci

Rhun

Charpentier

2012

RNA Biology

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Common Regulators of Virulence in Streptococci

Patenge

Fiedler

Kreikemeyer

2012

Host-Pathogen Interactions in Streptococcal Diseases

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Streptococcal species are a diverse group of bacteria which can be found in animals and humans. Their interactions with host organisms can vary from commensal to pathogenic. Many of the pathogenic species are causative agents of severe, invasive infections in their hosts, accounting for a high burden of morbidity and mortality, associated with high economic costs in industry and health care. Among them, Streptococcus pyogenes, Streptococcus agalactiae, Streptococcus pneumoniae, and Streptococcus suis are discussed here. An environmentally stimulated and tightly controlled expression of their virulence factors is of utmost importance for their pathogenic potential. Thus, the most universal and widespread regulators from the classes of stand-alone transcriptional regulators, two-component signal transduction systems (TCS), eukaryotic-like serine/threonine kinases, and small noncoding RNAs are the topic of this chapter. The regulatory levels are reviewed with respect to function, activity, and their role in pathogenesis. Understanding of and interfering with transcriptional regulation mechanisms and networks is a promising basis for the development of novel anti-infective therapies.

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Group A Streptococcal rofA Gene Is Involved in the Control of Several Virulence Genes and Eukaryotic Cell Attachment and Internalization

Cited by 69 publications

References 36 publications

Group A streptococcal RofA-type global regulators exhibit a strain-specific genomic presence and regulation pattern

Group A streptococcal RofA-type global regulators exhibit a strain-specific genomic presence and regulation pattern

Small RNAs in streptococci

Common Regulators of Virulence in Streptococci

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