Group 3 Late Embryogenesis Abundant Proteins in Desiccation-Tolerant Seedlings of Wheat (Triticum aestivum L.)

Ried, Jeffrey L.; Walker-Simmons, M. K.

doi:10.1104/pp.102.1.125

Cited by 107 publications

(76 citation statements)

References 24 publications

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“…Data from a number of previous studies indicate that accumulation of group 3 LEA proteins are correlated with stress tolerance (Ried and Walker-Simmons, 1993;Moons et al, 1995). On the other hand, the presence of other LEA proteins is not always correlated with stress tolerance.…”

Section: Discussionmentioning

confidence: 96%

“…For example, in severely dehydrated wheat seedlings, the accumulation of high lev-els of group 3 LEA proteins was correlated with tissue dehydration tolerance (Ried and Walker-Simmons, 1993). Studies of several Indica varieties of rice (Oryza sativa L.) showed that the levels of group 2 LEA proteins (also known as dehydrins) and group 3 LEA proteins in roots were significantly higher, or induced by ABA and salt stress, only in salt-tolerant varieties as compared with saltsensitive varieties (Moons et al, 1995).…”

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confidence: 99%

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Expression of a Late Embryogenesis Abundant Protein Gene, HVA1, from Barley Confers Tolerance to Water Deficit and Salt Stress in Transgenic Rice

et al. 1996

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A late embryogenesis abundant (LEA) protein gene, HVA7, from barley (Hordeum vulgare L.) was introduced into rice suspension cells using the Biolistic-mediated transformation method, and a large number of independent transgenic rice (Oryza sativa L.) plants were generated. Expression of the barley HVA 7 gene regulated by the rice actin 1 gene promoter led to high-level, constitutive accumulation of the HVAl protein in both leaves and roots of transgenic rice plants. Second-generation transgenic rice plants showed significantly increased tolerance to water deficit and salinity. Transgenic rice plants maintained higher growth rates than nontransformed control plants under stress conditions. The increased tolerance was also reflected by delayed development of damage symptoms caused by stress and by improved recovery upon the removal of stress conditions. We also found that the extent of increased stress tolerante correlated with the leve1 of the HVAl protein accumulated in the transgenic rice plants. Using a transgenic approach, this study provides direct evidence supporting the hypothesis that LEA proteins play an important role in the protection of plants under wateror salt-stress conditions. Thus, LEA genes hold considerable potentia1 for use as molecular tools for genetic crop improvement toward stress tolerance.

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Section: Discussionmentioning

confidence: 96%

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confidence: 99%

Expression of a Late Embryogenesis Abundant Protein Gene, HVA1, from Barley Confers Tolerance to Water Deficit and Salt Stress in Transgenic Rice

et al. 1996

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“…During germination, some lea proteins persist at high levels until 0.5 to 1 DPI [ 31 ]. A recent report showed that, in wheat seedlings, expression of lea proteins during early germination correlates with their ability to tolerate more than 90~o of water loss [32]. In preliminary desiccation tolerance experiments performed in our lab, sunflower 2-3 DPI seedlings were able to withstand water losses of 80~o; and to recover growth upon dehydration in almost 100~o of the cases.…”

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confidence: 83%

Expression of sunflower low-molecular-weight heat-shock proteins during embryogenesis and persistence after germination: localization and possible functional implications

1994

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We isolated and sequenced Ha hsp17.9, a DNA complementary (cDNA) of dry-seed stored mRNA that encodes a low-molecular-weight heat-shock protein (LMW HSP). Sequence analysis identified Ha hspl7.9, and the previously reported Ha hspl7.6, as cDNAs encoding proteins (HSP17.6 and H S P 17.9) which belong to different families of cytoplasmic LMW H S Ps. Using specific antibodies we observed differential expression of both proteins during zygotic embryogenesis under controlled environment, and a remarkable persistence of these LMW HSPs during germination. Immuno-blot analysis of HSP17.9 proteins in two-dimensional gels revealed that the polypeptides expressed in embryos were indistinguishable from LMW HSPs expressed in vegetative tissues in response to water deficit; but they appeared different from homologeous proteins expressed in response to thermal-stress. Tissue-print immunolocalization experiments showed that HSP17.9 and HSP17.6 were homogeneously distributed in every tissue of desiccation-tolerant dry seeds and young seedlings under non-stress conditions. These results demonstrate developmental regulation of specific, cytoplasmic, plant LMW HSPs, suggesting also their involvement in water-stress tolerance.

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“…The relevance of the early appearance of the B15C transcript is, however, dependent on whether the transcript is translated or not, since it has been shown, e.g. in the case of Group 3 Lea genes in wheat, that transcripts, but not the corresponding translation products, in some cases accumulate (Ried and Walker-Simmons, 1993). Clearly, the elucidation of the function of B15C during somatic embryogenesis and in seed dormancy requires that the putative protein encoded by the B15C ORF is identified and studied.…”

Section: B15c May Coordinate Dormancy In the Aleurone Layer And In Thmentioning

confidence: 99%

Transcripts encoding an oleosin and a dormancy‐related protein are present in both the aleurone layer and the embryo of developing barley (Hordeum vulgare L.) seeds

Aalen¹,

et al. 1994

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SummaryIn cereal seeds, the aleurone layer and the embryo share several characteristics, including synthesis and accumulation of lipid bodies, desiccation tolerance and dormancy. A number of Balem transcripts present in both the barley deurone layer and the embryo have been cloned by differential screening of a cDNA library from aleurone layers of immature barley grains. The Balem clones constitute two subgroups, one for which the transcripts are detectable in aleurone layers and embryos of developing seeds only (B23D and Bl5C), and another for which transcripts are present also in germinating embryos and in maternal tissues (B12D, B14E and 831 E). Sequence analysis identified 8230 and B15C as the barley homologues of the 18 kDa oleosin of maize embryos (72% amino acid identity) and the dormancy-associated transcript pBS128 from Bromus secalinus (95% identity), respectively. In sifu hybridization experiments demonstrate that in the embryo, the B23D transcript is mainly present in the scutellum, whereas the B15C transcript is predominantly present in shoot and root apices. Using anther-derived embryos and embryogenic cell suspensions, it is demonstrated that the B23D and B15C transcripts can be used as molecular markers for somatic embryogenesis. The functions of the transcripts in the second Balem subgroup remain unknown. Further studies on the Balem transcripts may shed light on the molecular basis for the extensive similarities between the embryo and the aleurone layer of the endosperm in the grass family.

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Group 3 Late Embryogenesis Abundant Proteins in Desiccation-Tolerant Seedlings of Wheat (Triticum aestivum L.)

Cited by 107 publications

References 24 publications

Expression of a Late Embryogenesis Abundant Protein Gene, HVA1, from Barley Confers Tolerance to Water Deficit and Salt Stress in Transgenic Rice

Expression of a Late Embryogenesis Abundant Protein Gene, HVA1, from Barley Confers Tolerance to Water Deficit and Salt Stress in Transgenic Rice

Expression of sunflower low-molecular-weight heat-shock proteins during embryogenesis and persistence after germination: localization and possible functional implications

Transcripts encoding an oleosin and a dormancy‐related protein are present in both the aleurone layer and the embryo of developing barley (Hordeum vulgare L.) seeds

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