In this article, we explore properties of pseudo entropy [1] in quantum field theories and spin systems from several approaches. Pseudo entropy is a generalization of entanglement entropy such that it depends on both an initial and final state and has a clear gravity dual via the AdS/CFT. We numerically analyze a class of free scalar field theories and the XY spin model. This reveals basic properties of pseudo entropy in quantum many-body systems, namely, the area law behavior, the saturation behavior, and the non-positivity of difference between the pseudo entropy and averaged entanglement entropy in the same quantum phase. In addition, our numerical analysis finds an example where the strong subadditivity of pseudo entropy gets violated. Interestingly, we find that the non-positivity of the difference can be violated only if the initial and final state belong to different quantum phases. We also present analytical arguments which support these properties by both conformal field theoretic and holographic calculations. When the initial and final state belong to different topological phases, we expect a gapless mode localized along an interface, which enhances the pseudo entropy, leading to the violation of the non-positivity of the difference. Moreover, we also compute the time evolution of pseudo entropy after a global quantum quench, where we observe that the imaginary part of pseudo entropy shows an interesting characteristic behavior.