Gross Morphological, Histological and Histochemical Studies on Liver of Pati Ducks (Anas platyrhynchos domesticus) of Assam

Abstract

Background: Pati ducks have been playing a vital role in Assam’s rural economy. It is an established fact that the liver is an important organ in the body. Since literature on a detailed anatomical study of the liver in Pati ducks of Assam is scare, hence the present study was designed to establish the gross, histological and histochemical details on liver with advancement of age. Methods: The present study was conducted on 24 numbers of Pati ducks of Assam. They were divided into four (4) groups, each group containing six birds and kept for different ages i.e. 1 week, 8 weeks, 16 weeks and 24 weeks. The gross morphometry viz. length, breadth, thickness and weight of liver were observed. For histological study, tissue samples were fixed in 10% neutral buffered formalin. The processing and various methods staining were used according to Luna (1968). For histochemical study, samples were stored at -80°C in a deep freeze immediately after collection and they were treated according to Singh and Sulochana’s method (1978). Result: Morphometrical parameters showed an increasing trend with age. The lobules of liver were not distinct. Strong Alkaline phosphatase and Acid phosphatase activity were observed in the capsular area and bile canaliculi respectively.