Grosmannia tibetensis, a new ophiostomatoid fungus associated with Orthotomicus sp. (Coleoptera) in Tibetan subalpine forests

Wang, Zheng; Ya, Liu; Wang, Tian Tian; Decock, Cony; Chu, Biao; Zheng, Qing; Lü, Quan; Zhang, Xing Yao

doi:10.1016/j.myc.2020.05.004

Cited by 2 publications

(2 citation statements)

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“…The CAL primer pair, CALngsF1 and CALngsR1 (Figure 1b), amplifies a length variable amplicon (193–417 bp) spanning the 4th to 6th exon of calmodulin (Yin et al, 2015). The BT region was excluded from further analysis here due to evidence of paralogous copies present in the Grosmannia penicillata (Grosmann) and R. arxii genomes (data not shown), suggesting the paralogy may extend beyond the Grosmannia genus (Wang et al, 2020).…”

Section: Resultsmentioning

confidence: 99%

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Modular, multi‐barcode amplicon sequencing for improved species‐level detection of fungal phytopathogens: A case study of pipeline establishment targeting the Ophiostomatales

et al. 2022

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Recent advances in the field of environmental DNA (eDNA) metabarcoding have produced a promising platform for early detection and broad‐spectrum monitoring of plant pests and pathogens. To date, the majority of fungal metabarcoding assays have relied upon universal primers amplifying segments of the ribosomal DNA, to ensure broad taxonomic coverage while studying changes in community composition, or more recently, to screen for presence/absence of target species. In a diagnostics framework however, single, universal molecular barcodes do not discriminate accurately enough for many groups of important fungal phytopathogens at the required species level. Here, a modular, multi‐barcode amplicon sequencing pipeline was established to provide rapid and reliable diagnostics targeting the Ophiostomatales, an order containing economically important phytopathogens vectored by bark and ambrosia beetles (Curculionidae). Using compositionally varied mock communities, we evaluated five barcoding loci: ITS1, ITS2, the large ribosomal sub‐unit (LSU), translation elongation factor 1‐alpha (TEF1α) and calmodulin (CAL), for their ability to provide species‐level resolution. The sensitivity and detection limit of the assay was established by spiking genomic DNA of the exotic Dutch elm disease pathogen, Ophiostoma novo‐ulmi Brasier, into both the controlled mock communities and eDNA sampled from Ips grandicollis (Eichhoff) beetles collected in New South Wales, Australia. The TEF1α barcode successfully detected the Dutch elm pathogen DNA with 100% accuracy down to approx. 5 pg/μl, while the ITS1 barcode had a 100% accuracy down to approx. 0.4 pg/μl within a given sample. Our results demonstrate that a dual‐barcode approach targeting the ITS1 and TEF1α regions simultaneously is sufficient for accurate species level detection and characterisation of the Ophiostomatales and offers confident detection of low‐abundance taxa (relative read abundances of 0.1%–0.01%). Multi‐barcode metabarcoding provides a framework which can quickly and efficiently be adapted to new targets when establishing high throughput diagnostics for post‐border biosecurity surveillance of fungal phytopathogens.

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Section: Resultsmentioning

confidence: 99%

“…and R. arxii genomes (data not shown), suggesting the paralogy may extend beyond the Grosmannia genus (Wang et al, 2020).…”

Section: Barcode Assessment and Primer Designmentioning

confidence: 99%

Modular, multi‐barcode amplicon sequencing for improved species‐level detection of fungal phytopathogens: A case study of pipeline establishment targeting the Ophiostomatales

et al. 2022

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Morphological and Phylogenetic Analyses Reveal a New Species of Ceratocystiopsis (Ophiostomataceae, Ophiostomatales) Associated with Ips subelongatus in Inner Mongolia (China) with Weak Host Pathogenicity

Wang

Liu

et al. 2021

Forests

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Ophiostomatoid fungi are known for their associations with bark beetles, and some species are important sources of tree diseases. Ceratocystiopsis is a genus of the ophiostomatoid fungi in order Ophiostomatales. The shortage of DNA barcodes for many species in this genus has resulted in the presence of many unnamed cryptic species. In this study, Ceratocystiopsis subelongati sp. nov. associated with Ips subelongatus infesting Pinus sylvestris var. mongolica in Inner Mongolia, China, was identified and described based on phylogenetic inference of multi-gene DNA sequences and morphological characteristics. The species is characterized by a hyalorhinocladiella- to sporothrix-like asexual state and an optimal growth temperature of 30 °C. Artificial inoculation tests in the field showed that it is mildly pathogenic to five-year-old larch trees, the main host of I. subelongatus. It is also the first described Ceratocystiopsis species associated with I. subelongatus in China. This discovery should provide new avenues for studying the symbiosis between bark beetles and ophiostomatoid fungi.

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Grosmannia tibetensis, a new ophiostomatoid fungus associated with Orthotomicus sp. (Coleoptera) in Tibetan subalpine forests

Cited by 2 publications

References 41 publications

Modular, multi‐barcode amplicon sequencing for improved species‐level detection of fungal phytopathogens: A case study of pipeline establishment targeting the Ophiostomatales

Modular, multi‐barcode amplicon sequencing for improved species‐level detection of fungal phytopathogens: A case study of pipeline establishment targeting the Ophiostomatales

Morphological and Phylogenetic Analyses Reveal a New Species of Ceratocystiopsis (Ophiostomataceae, Ophiostomatales) Associated with Ips subelongatus in Inner Mongolia (China) with Weak Host Pathogenicity

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