Grooming analysis algorithm for neurobehavioural stress research

Kalueff, Allan V.; Tuohimaa, Pentti

doi:10.1016/j.brainresprot.2004.04.002

Cited by 234 publications

(159 citation statements)

References 26 publications

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“…The grooming observed was characterized by short, interrupted spurts, consistent with stress-evoked stereotypy rather than low-stress comfort grooming. 57,58 Anxiety-like avoidance behavior was also tested in the elevated plus maze using adult male C57BL/6 mice from Taconic (Hudson, NY) at 2−3 months of age. Following the 5-day social defeat paradigm, intruders and behavioral controls were tested on day 6 for anxiety-like behavior in the elevated plus maze.…”

Section: ■ Methodsmentioning

confidence: 99%

Social Stress Alters Inhibitory Synaptic Input to Distinct Subpopulations of Raphe Serotonin Neurons

Crawford

Rahman

Beck

2013

ACS Chem. Neurosci.

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Anxiety disorders are among the most prevalent psychiatric disorders, yet much is unknown about the underlying mechanisms. The dorsal raphe (DR) is at the crux of the anxiety-inducing effects of uncontrollable stress, a key component of models of anxiety. Though DR serotonin (5-HT) neurons play a prominent role, anxiety-associated changes in the physiology of 5-HT neurons remain poorly understood. A 5-day social defeat model of anxiety produced a multifaceted, anxious phenotype in intruder mice that included increased avoidance behavior in the open field test, increased stress-evoked grooming, and increased bladder and heart weights when compared to control mice. Intruders were further compared to controls using electrophysiology recordings conducted in midbrain slices wherein recordings targeted 5-HT neurons of the ventromedial (vmDR) and lateral wing (lwDR) subfields of the DR. Though defining membrane characteristics of 5-HT neurons were unchanged, γ-aminobutyric-acid-mediated (GABAergic) synaptic regulation of 5-HT neurons was altered in a topographically specific way. In the vmDR of intruders, there was a decrease in the frequency and amplitude of GABAergic spontaneous inhibitory postsynaptic currents (sIPSCs). However, in the lwDR, there was an increase in the strength of inhibitory signals due to slower sIPSC kinetics. Synaptic changes were selective for GABAergic input, as glutamatergic synaptic input was unchanged in intruders. The distinct inhibitory regulation of DR subfields provides a mechanism for increased 5-HT output in vmDR target regions and decreased 5-HT output in lwDR target regions, divergent responses to uncontrollable stress that have been reported in the literature but were previously poorly understood.

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Section: ■ Methodsmentioning

confidence: 99%

Social Stress Alters Inhibitory Synaptic Input to Distinct Subpopulations of Raphe Serotonin Neurons

Crawford

Rahman

Beck

2013

ACS Chem. Neurosci.

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“…Different multivariate approaches are available to assess animal behavior [5,8,10,11,15,17]. In present research we used a MVA based on transition matrices elaborations.…”

Section: Multivariate Analysesmentioning

confidence: 99%

“…It is our contention that contrasting findings concerning this behavioral component have been produced by the unfortunate association of two factors: first, since HB introduction almost 50 years ago [1], inter-relations among behavioral components in this experimental assay have been scantly investigated; second, as a consequence and even more important, only quantitative evaluations of headdip have been produced for a long time. Multivariate analyses (MVA) provide excellent tools to overcome such constraints because they make available useful and interesting information on the relationships among behavioral elements [3,4,5,10,11,15,17]. Concerning studies on anxiety and depression, various articles have provided, by means of MVA, reliable information on rodent's behavior in the elevated-plus maze [9], in the forced swimming test [12], in the sucrose preference test and in the open field [2].…”

Section: Introductionmentioning

confidence: 99%

“…On this subject, remarkable information has been provided, in the past, by analyzing the behavioral microstructure of different rodent's activities, such as grooming [11] or feeding [20]. The aim of the present research is to describe, from a methodological point of view, an alternative approach to the study of rodent behavior in HB, by focusing the analysis on holeexploration activity in its microstructural complexity.…”

Section: Introductionmentioning

confidence: 99%

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Microstructural assessment of rodent behavior in the hole-board experimental assay

Casarrubea

Sorbera

Santangelo

et al. 2010

Proceedings of the 7th International Conference on Methods and Techniques in Behavioral Research

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The aim of the present methodological article was to assess whether a microstructure of hole-exploratory activity can be demonstrated in rats tested in the hole-board, an experimental assay widely used to study anxiety-related behaviors in rodents. Six never properly described holeexploratory behavioral components were identified: activedip, static-dip, rapid-dip, circular-sniff, point-sniff and central-sniff. Results, assessed by means of descriptive and multivariate approaches, reveal a complex microstructural organization of these behavioral elements. The hypothesis of an important role of the relationships between point-sniff and head dipping activities, in anxiety-related holeexploratory behavior, is proposed. It is suggested that the microstructural analysis can evidence even subtle behavioral changes induced by manipulations of animal anxiety condition.

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“…Also, MVA has been utilized to illustrate different features of rat responses in the elevated plus-maze (Cruz, Frei, & Graeff, 1994;Espejo, 1997), in the hot plate (Espejo & Mir, 1993, and in the forced swimming test (Lino-de-Oliveira, De Lima, & Carobrez, 2005). By means of such methods, animal grooming activities ( Kalueff & Tuohimaa, 2004), aggressiveness-related responses (Aguilar et al, 2002;Aguilar et al, 2003;Mos, Olivier, & van der Poel, 1987), and social (Van Den Berg, Van Ree, & Spruijt, 1999) and genetic (Takahashi, Kato, Makino, Shiroishi, & Koide, 2006) influences on behavior have been described as well. In an interesting article, Hemerik, Bukovinszky, Gols, van Lenteren, and Vet (2006) proposed a thoughtful depiction of a specific multivariate approach in the study of parasitoid foraging behavior.…”

mentioning

confidence: 99%