Griseococcin(1) from Bovistella radicata (Mont.) Pat and antimicrobial activity

Abstract

Background: To evaluate the antifungal activity of B. radicata fermentation broth, the broth was purified by DEAE-cellulose and sephadex LC-20 column. The compounds were submitted to spectral analysises(HPLC, FT-IR, 1D and 2D NMR etc.). Results: The purified compounds were identified as the Griseococcin(s) which were naphthoquinone derivatives, only Griseococcin(1) has antifungal activity among the Griseococcin(s). Minimum inhibitory concentration(MIC) and zone of inhibition(ZOI) of Griseococcin(1) were 31.2, 31.2 μg/ml and 18.06±0.85 mm, 15.01±1.02 mm respectively against main pathogenic fungus Trichophyton rubrum and Trichophyton mentagrophytes, the Chemical formula and MW of Griseococcin(1) was determined as C37O10H43N and 661Da.Conclusions: In this paper, the secondary metabolite compound Griseococcin(1) from B. radicata was purified. The purified compound can restrain main pathogens(T. rubrum and T. mentagrophytes) leading to tinea pedis. The antifungal activity of Griseococcin(1) was similar to that of the positive control.