GRIM-19 Expression and Function in Human Gliomas

Jin, Yongdong; Jung, Shin; Jin, Shu‐Guang; Jung, Tae‐Young; Moon, Kyung‐Sub; Kim, In-Young

doi:10.3340/jkns.2010.48.1.20

Cited by 6 publications

(2 citation statements)

References 43 publications

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“…The GeneFishing technique allows the detection of genes differentially expressed between two or more RNA samples and has been used extensively to isolate genes related to cancer, apoptosis, and immune responses Jin et al, 2010). We demonstrated that this technology can also be applied to insect systems; using this technology, we identified βGRP and attacinlike, alcohol-dehydrogenase-like, lipid-transport-like, 3oxoacyl-synthase-like, esterase-like, and ribosomal-like genes in the beet armyworm, a major pest species.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a β‐1,3‐glucan recognition protein from the beet armyworm, Spodoptera exigua (Insecta: Lepidoptera: Noctuidae)

2012

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The β-1,3-glucan recognition protein gene from Spodoptera exigua (SeβGRP) was cloned and characterized. The cDNA of this gene is 1 644 nucleotides in length and the predicted polypeptide is 491 amino acids (aa) in length, with a calculated molecular mass of 54.8 kDa. The first 22 aa encode a predicted secretion signal peptide. A BLAST search, multiple sequence alignment, and phylogenetic analysis of the aa sequence of SeβGRP revealed that this protein is most similar to the β-1,3-glucan recognition protein (βGRP) family of pattern recognition proteins. Using reverse-transcription polymerase chain reaction, we detected the presence of SeβGRP transcripts in the egg, larval, pupal, and adult stages of S. exigua. In addition, the SeβGRP transcript was expressed in all the tissues examined including the brain, hemocytes, fat body, intestine, and cuticle. There were no changes in SeβGRP mRNA levels in larvae infected with ultraviolet (UV)-killed Escherichia coli DH5α compared with the control larvae inoculated with the water; however, SeβGRP mRNA levels were markedly elevated 4-8 h after infection and slightly induced 12-24 h after infection in larvae injected with UV-killed Fusarium oxysporum. This may be because β-1,3-glucan is the main component of the cell wall of F. oxysporum, but not E. coli DH5α.

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Section: Discussionmentioning

confidence: 99%

Characterization of a β‐1,3‐glucan recognition protein from the beet armyworm, Spodoptera exigua (Insecta: Lepidoptera: Noctuidae)

2012

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“…In humans, GRIM-19 encodes a 16-kDa protein of 144 amino acids [9]. The GRIM-19 protein contains no conserved domain structures and motifs.…”

Section: Introductionmentioning

confidence: 99%

Expression and functional characterization of a gene associated with retinoid-interferon-induced mortality 19 (GRIM-19) from orange-spotted grouper (Epinephelus coioides)

Shi

Zhao

Zhu

et al. 2013

Fish & Shellfish Immunology

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Characterization and expression of attacin, an antibacterial protein-encoding gene, from the beet armyworm, Spodoptera exigua (Hübner) (Insecta: Lepidoptera: Noctuidae)

et al. 2011

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To isolate antimicrobial-related genes from the beet armyworm, Spodoptera exigua, we performed GeneFishing, a polymerase chain reaction (PCR)-based differential display technique. An attacin-like complementary DNA (cDNA) including a 3'-untranslated region was identified from among 18 over-expressed genes in microbial-infected larvae. The full-length attacin cDNA from S. exigua cDNA (Seattacin) was cloned using rapid amplification of cDNA ends PCR. The attacin-like cDNA transcript was 765 nucleotides in length, and the predicted polypeptide was 254 amino acids in length with a calculated molecular mass of 27.6 kDa and an isoelectric point of 6.44. The protein sequence of the attacin-like cDNA showed high identity to that of Trichoplusia ni (61.2%). The amino acid sequence identity of Seattacin to the orthologous proteins in Bombyx mori, Manduca sexta, Heliothis virescens, Hlicoverpa armigera, Hyphantria cunea, Hyalophora cecropia, and Drosophila melanogaster was 61.2, 46.1, 44.5, 42.2, 39.5, 45.1, and 24.0%, respectively. To examine possible immune functions of the attacin-like cDNA, its expression was investigated by reverse transcriptase PCR analysis after challenging S. exigua with microorganisms. The attacin-like cDNA was expressed at high levels 12 h post-infection, and its expression was slightly induced 4-8 h post-infection compared to control larvae inoculated with sterile water. Furthermore, induced Seattacin showed biological activity against several bacteria including Escherichia coli DH5α, Pseudomonas cichorii, Bacillus subtilis, and Listeria monocytogenes. These results suggest that the attacin-like cDNA of S. exigua codes for antimicrobial peptides.

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GRIM-19 Expression and Function in Human Gliomas

Cited by 6 publications

References 43 publications

Characterization of a β‐1,3‐glucan recognition protein from the beet armyworm, Spodoptera exigua (Insecta: Lepidoptera: Noctuidae)

Characterization of a β‐1,3‐glucan recognition protein from the beet armyworm, Spodoptera exigua (Insecta: Lepidoptera: Noctuidae)

Expression and functional characterization of a gene associated with retinoid-interferon-induced mortality 19 (GRIM-19) from orange-spotted grouper (Epinephelus coioides)

Characterization and expression of attacin, an antibacterial protein-encoding gene, from the beet armyworm, Spodoptera exigua (Hübner) (Insecta: Lepidoptera: Noctuidae)

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