Griffonia simplicifolia (DC.) Baill. attenuates gentamicin and cisplatin-induced nephrotoxicty in rats

Nyarko, Ruby; Larbie, Christopher; Anning, Alexander K.; Baidoo, P. K.; Emikpe, Benjamin Obukowho; Oyagbemi, Ademola Adetokunbo; Jarikre, Theophilus Aghogho

doi:10.1007/s00580-019-02934-x

Cited by 7 publications

(8 citation statements)

References 32 publications

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“…The immunohistochemical staining was performed using the streptavidin-biotin peroxidase complex kit (M IHC Select Detection System, HRP/DAB, Merck, Germany; Lot: 2775482) with a slight modification of the procedure as previously described by Jarikre and Emikpe 13 and also used previously. 14 Primary antibodies used were monoclonal mouse nuclear factor-κβ (NF-κβ) and interleukin 1 (IL-1) at a dilution of 1:100 in phosphate-buffered saline. Tissue sections from the CCl 4 and Para only treated rats served as negative controls.…”

Section: Methodsmentioning

confidence: 99%

Modulation of Oxidative Stress and Inflammatory Cytokines as Therapeutic Mechanisms ofOcimum americanumL Extract in Carbon Tetrachloride and Acetaminophen-Induced Toxicity in Rats

Genfi

Larbie

Emikpe

et al. 2020

J Evid Based Complementary Altern Med

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Liver diseases have now become a global canker due to increasing drug abuse and several viral infections. The current medicines on the market are woefully inadequate and limited in the application against these diseases. Fortunately, medicinal plants continue to serve as a potential source of drug discovery that could be explored to improve the situation. The present study, therefore, evaluated the hepatoprotective activities of the aqueous extract of various parts (leaves, flower and stem) of Ocimum americanum L on carbon tetrachloride (CCl4)- and acetaminophen-induced toxicity in rats. The protective effect of the plant was assessed using biochemical parameters, histology, levels of liver antioxidants, and expression of some pro-inflammatory cytokines (NF-κβ and IL-1) in the liver. The leaves and stem extracts, orally administered for 7 days at 250 mg/kg, effectively prevented CCl4-induced elevation of serum biochemical parameters, prooxidants, as well as the expression of NFk-B and IL-1, which were comparable to Silymarin (standard drug). A comparative histopathological analyses of the liver exhibited virtually normal architecture compared with CCl4-treated group. The findings showed that the hepatoprotective effect of Ocimum americanum was probably due to the inhibition of oxidative stress and downregulation of proinflammatory cytokines by the effective parts of the medicinal plant.

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Section: Methodsmentioning

confidence: 99%

Modulation of Oxidative Stress and Inflammatory Cytokines as Therapeutic Mechanisms ofOcimum americanumL Extract in Carbon Tetrachloride and Acetaminophen-Induced Toxicity in Rats

Genfi

Larbie

Emikpe

et al. 2020

J Evid Based Complementary Altern Med

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“…The immunohistochemical staining was performed using the Streptavidin-biotin peroxidase complex kit (M IHC Select Detection System, HRP/DAB, Merck, Germany LOT: 2775482) with a slight modification of the procedure, as previously described by Jarikre and Emikpe 21 and previously used 22 . Rabbit monoclonal to NF-kB (2A12A7), COX-1 (19999, Lot # L1113), IL-17 (SAB3701439, Lot R127357), and IL-23 (Cat.…”

Section: Immunohistochemical Analysismentioning

confidence: 99%

Acalypha wilkesiana ‘inferno’ hydroethanolic leaf extract has protective effect on carbon tetrachloride-induced subacute toxicity in animals

et al. 2020

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Introduction: Liver fibrosis is one of the most common clinical manifestations of hepatic diseases. However, though many synthetic drugs exist for the treatment and prevention of liver diseases, liver injuries still persist. The present study, therefore, sought to investigate the subacute protective effects of Acalphya wilkesiana against carbon tetrachloride (CCl4)-induced toxicity in animals. Methodology: Liver injury was induced in experimental animals by administering CCl4 (1:1 v/v in olive oil, intraperitoneally (i.p.), twice weekly for 8 weeks) after pre-treatment with extract of A. wilkesiana (AWE). AWE (250 mg/kg) and Silymarin (120 mg/kg) were administered orally (daily for 8 weeks). The hepatoprotective effect was studied by assaying the activity of liver enzymes, such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and alpha-fetoprotein. The effect of the treatments on liver prooxidants (e.g. malondialdehyde [MDA]) and antioxidants (e.g. superoxide dismutase [SOD], reduced glutathione [GSH], glutathione peroxidase [GPx], and glutathione transferase [GST]), as well as inflammatory cytokines (e.g. interleukin [IL]-17, IL-23, nuclear factor kappa beta [NF-kB], and cycloxygenase-1 [COX-1]) and the histology of the liver were also examined. Results: The activity of liver function biomarkers changed significantly upon CCl4 administration; increases in ALT, total and direct bilirubin, and some fibrosis indices (e.g. alpha-fetoprotein and APRI [p<0.05-0.001, compared with normal]) were observed. Co-administration of AWE with CCl4 restored these to normal levels. The intensity of structural alterations revealed that the AWE treatment has protective potential against subacute liver injury. AWE treatment also reduced the expression of IL-17, 1L-23, NF-kB and COX-1, underscoring its antiinflammatory properties. Conclusion: The results of the current study generally suggest that hydroethanolic leaf extracts of A. wilkesiana inferno possess some subacute protective activity by improving liver function and inhibition of inflammation, and could be developed as a potent antifibrotic agent.

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“…Drug-induced Nephrotoxicity: Gentamicin (GM, Letpa Pharmaceuticals Limited, Accra, Ghana; 120 mg/kg body weight, administered intramuscularly) and cisplatin (Cisp, Celon Labs, India; 7 mg/kg administered intraperitoneally) were used to induce renal injury in the experimental animals. GM was administered from the 2nd to 7th days of the experiment while Cisp was administered on the 3rd day according to the procedure described by Chatterjee et al [23] with the previously used modifications [14].…”

Section: Plant Preparation and Extractionmentioning

confidence: 99%

“…Immunohistochemistry for expression of pro-inflammatory markers: Streptavidin-biotin peroxidase complex kit (M IHC Select Detection System, HRP/DAB, Merck, Germany, Lot: 2775482) was used for the immunohistochemical staining, the procedure of which described by Jarikre and Emikpe [31] and also used previously [14]. The primary antibodies were interleukin (IL) 17 (SAB3701439, Lot: R127357), monoclonal mouse cyclooxygenase (COX) antibody (sc-19999, Lot # L1113), and interleukin IL 23 (Cat.…”

Section: ×100% Absolute Organ Weight Body Weight At Sacrificementioning

confidence: 99%

“…Traditionally, herbs have been used for the treatment of renal disorders induced by drugs and numerious toxins. In this context, many medicinal plants have been tested for their nephroprotective effects in nephrotoxicities induced by GM and Cisp [11][12][13][14]. Medicinal plants are used as curatives due to their active components with medicinal value.…”

Section: Introductionmentioning

confidence: 99%

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Acalypha wilkesiana ‘Inferno’ Is Nephroprotective Against Gentamicin and Cisplatin: Biochemical, Histological and Immunohistochemical Evidence

Larbie

Emikpe

Oyagbemi

et al. 2020

IJT

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Background: Kidneys exposure to toxins can cause injuries, leading to their functional impairments. Traditionally, plants have been used for the treatment of renal disorders and numerous medicinal plants have been tested for their nephroprotective effects, in such cases as gentamicin (GM) and cisplatin (Cisp)-induced nephrotoxicity. This study assessed the ability of Acalypha wilkesiana’s extract to counteract its toxic effect based on the biochemical, histological and proinflammatory cytokines components in rats. Methods: Thirty-six male Wistar rats were randomly divided into nine groups (n=4 each) and administered the following treatments: a) normal control (1 mL/kg body weight normal saline from days 1-10); b) nephrotoxin (GM 120 mg/kg, days 2-7; or Cisp 7 mg/kg on day 3); c) standard drug (120 mg/kg Silymarin plus GM or Cisp, days 1-10); and, d) extract groups (100 or 250 mg/kg, days 1-10 plus GM). Blood samples were collected and subjected to hematological and biochemical evaluations while kidney tissue samples were examined for histopathological alterations, pro- and antioxidants, and expression of pro-inflammatory cytokines. Results: Treatment of the rats pre-exposed to GM or Cisp with the extract decreased the serum creatinine, urea and MDA levels. The GST and GPx levels were also restored in rats. Glomerular atrophy with tubular epithelial necrosis induced by either nephrotoxin was restored to near normal. The expression of COX-2 following the administration of either nephrotoxin was reversed after treatment with the extract. Conclusion: The A. wilkesiana extract exhibited significant nephroprotective property, which could potentially be regarded as a promising alternative to the management of renal diseases.

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Griffonia simplicifolia (DC.) Baill. attenuates gentamicin and cisplatin-induced nephrotoxicty in rats

Cited by 7 publications

References 32 publications

Modulation of Oxidative Stress and Inflammatory Cytokines as Therapeutic Mechanisms ofOcimum americanumL Extract in Carbon Tetrachloride and Acetaminophen-Induced Toxicity in Rats

Modulation of Oxidative Stress and Inflammatory Cytokines as Therapeutic Mechanisms ofOcimum americanumL Extract in Carbon Tetrachloride and Acetaminophen-Induced Toxicity in Rats

Acalypha wilkesiana ‘inferno’ hydroethanolic leaf extract has protective effect on carbon tetrachloride-induced subacute toxicity in animals

Acalypha wilkesiana ‘Inferno’ Is Nephroprotective Against Gentamicin and Cisplatin: Biochemical, Histological and Immunohistochemical Evidence

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