Gridlock signalling pathway fashions the first embryonic artery

Zhong, Tao; Childs, Sarah J.; Leu, James; Fishman, Mark C.

doi:10.1038/35102599

Cited by 493 publications

(402 citation statements)

References 30 publications

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“…22 In this study, we report the analysis of the migration pathway of definitive HSPCs in zebrafish. Using cell labeling technique such as injection of 4,5-dimethoxy-2-nitrobenzyl (DMNB) caged fluorescein (flu) followed by local uncaging, 23,24 we found that flu-labeled cells in the ventral wall of DA at 30 hpf could become rag1-positive T cells, a hematopoietic lineage exclusively generated during definitive hematopoiesis in the thymus, confirming that the cells located in the ventral wall of DA represent the zebrafish counterparts of definitive HSPCs found in the mouse AGM. When the same methodology was applied, we were able to show the migration of definitive HSPCs from the ventral wall of DA to a previously unappreciated hematopoietic organ, the posterior blood island (PBI), located between the caudal artery and caudal vein, prior to populating the kidney.…”

Section: Introductionmentioning

confidence: 99%

Migratory path of definitive hematopoietic stem/progenitor cells during zebrafish development

Jin

Wen

2007

Blood

145

151

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The development of vertebrate definitive hematopoiesis is featured by temporally and spatially dynamic distribution of hematopoietic stem/progenitor cells (HSPCs). It is proposed that the migration of definitive HSPCs, at least in part, accounts for this unique characteristic; however, compelling in vivo lineage evidence is still lacking. Here we present an in vivo analysis to delineate the migration route of definitive HSPCs in the early zebrafish embryo. Cell-marking analysis was able to first map definitive HSPCs to the ventral wall of dorsal aorta (DA). These cells were subsequently found to migrate to a previously unappreciated organ, posterior blood island (PBI), located between the caudal artery and caudal vein, and finally populate the kidney, the adult hematopoietic organ. IntroductionThe ontogeny of vertebrate hematopoietic system is characterized by 2 phases of development: an early transitory primitive wave generating only primitive erythrocytes and some myeloid cells, and a definitive wave that initiates late and produces all the mature blood lineages (erythroid, myeloid, and lymphoid). [1][2][3][4] In vertebrates, the first hematopoietic activity can be identified in an extra-embryonic location: yolk sac in mice and chickens, or ventral blood island (VBI) in amphibians, leading to the hypothesis that yolk sac/VBI is the sole source of hematopoietic cells, which will in turn populate downstream hematopoietic compartments. 5 However, the singular hematopoietic origin from yolk sac/VBI is challenged by substantial evidence from experiments with various species that reveals another evolutionary conserved intraembryonic hematopoietic site: the para-aortic splanchnopleurae (pSP) and later referred as aorta-gonad-mesonephros (AGM). [6][7][8][9][10][11] Hence, it is currently believed that yolk sac/VBI mainly supports primitive hematopoiesis, whereas definitive hematopoiesis is autonomously initiated, at least in part, in the pSP/AGM region.The journey of definitive HSPCs is best characterized both temporally and spatially in mice. 4 Although multipotential hematopoietic progenitor cells revealed by in vitro colony assays, injection into yolk sac cavity, transplantation into fetal liver of newborn mice or reconstitution of immunodeficient mice, can be detected in the pSP/AGM region and yolk sac at earlier developmental stages, 12-15 the first definitive HSPCs, defined as cells fully competent to provide long-term reconstitution of irradiated adult recipients, emerge in the pSP/AGM region at embryonic day (E) 10.5. 6,9,11 Subsequently, at around E11.0, definitive HSPC activity can be found in yolk sac. 6,9,11 Recently, placenta is recognized as another embryonic reservoir from E10.5 for definitive HSPCs operation. 16,17 However, the cellular origin of definitive HSPCs in yolk sac and placenta and their contribution to adult hematopoiesis are not clear. From E11.5, fetal liver begins to be populated by definitive HSPCs and serves as the main site that supports hematopoietic expansion and differentiation dur...

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Section: Introductionmentioning

confidence: 99%

Migratory path of definitive hematopoietic stem/progenitor cells during zebrafish development

Jin

Wen

2007

Blood

145

151

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“…Notch also regulates fjx expression in mice and there is clear overlap with notch expression in some, but not all regions of fjx expression (Rock et al, 2005). Notch proteins, together with their ligands, are involved in the specification of the dorsal aorta in zebrafish whilst expression analyses have implicated notch signaling in chick limb arterial development (Vargesson et al, 1998;Lawson et al, 2001;Zhong et al, 2001). This is analogous to the expression of fjx in the developing limb arteries but not the veins.…”

Section: Discussionmentioning

confidence: 99%

Developmental expression of the chick four‐jointed homologue

Yamaguchi

Parish

Akita

et al. 2006

Developmental Dynamics

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Four-jointed is a type II transmembrane protein that is thought to be cleaved to give rise to a secreted protein. In Drosophila, four-jointed controls outgrowth, vein patterning, and bristle polarity in the developing limb together with the polarity of the ommatidia in the developing eye. In Drosophila and mice, Fj is regulated by notch signaling. Here, we have determined the expression of the chick four-jointed (fjx) homologue during embryonic development. We show that fjx is expressed in the limb bud; facial primordia; the proliferating zone of the lens, feather buds, the neural tube; and neural crest derivatives such as the dorsal root ganglia. Analysis of the fjx expression in the developing limb bud showed that initially fjx is expressed throughout the limb bud, but as the limb develops, highest levels of fjx transcripts are found distally. However, by stage 27, fjx expression is predominantly found in the central core of the limb bud. Finally, fjx expression becomes confined to the developing tendons, ligaments, articular cartilage, and arteries but not the veins. Comparison with scleraxis (scx), a marker of tendons and ligaments, revealed that they are coexpressed in the majority of tendons but that fjx is expressed after scx, when the tendons have begun to differentiate. These data suggest that fjx has two roles during limb development: the first controlling outgrowth and the second tissue differentiation. Developmental Dynamics 235:3085-3091, 2006.

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“…Toutes sont également exprimées dans le système nerveux où elles contrô-lent la destinée et le guidage des précur-seurs neuronaux et des axones, ce qui suggère leur rôle dans l'établissement du réseau vasculaire. Les CE artérielles du poisson zèbre, du poulet et de la souris expriment sélectivement l'éphrine-B2, la neuropiline 1 (NRP1), le récepteur notch3, le ligand de Notch Delta-like 4 et gridlock [5][6][7][8][9][10][11][9][10][11][12][13][14][15][16]. D'autres molécules sont spécifiquement exprimées par les CE veineuses, notamment EphB4, le récepteur du marqueur artériel éphrine-B2 [12].…”

Section: Les Marqueurs Moléculaires Spécifiques Des Artères Et Des Veunclassified

Différenciation artérioveineuse : génétique ou hémodynamique ?

2004

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> Le système vasculaire adulte comprend trois compartiments: artériel, veineux et lymphatique, mais jusqu'à récemment, le contrôle de l'identité de ces vaisseaux était mal connu. Au cours du développe-ment embryonnaire, la mise en place du réseau artérioveineux s'effectue très précocement, peu après les premiers battements cardiaques, afin d'assurer la circulation embryonnaire. La décou-verte récente de marqueurs artériels et veineux spécifiques pouvait laisser supposer une prédétermination des cellules endothéliales (CE) à participer soit à une artère, soit à une veine et, de ce fait, un destin irréversiblement fixé par des facteurs génétiques. En dépit de l'expression de ces gènes spécifiques, nous avons montré que les CE gardent une plasticité vis-à-vis de leur différen-ciation artérielle ou veineuse, et ce en relation avec des facteurs hémodyna-miques.

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Gridlock signalling pathway fashions the first embryonic artery

Cited by 493 publications

References 30 publications

Migratory path of definitive hematopoietic stem/progenitor cells during zebrafish development

Migratory path of definitive hematopoietic stem/progenitor cells during zebrafish development

Developmental expression of the chick four‐jointed homologue

Différenciation artérioveineuse : génétique ou hémodynamique ?

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