GreenTray® TIS bioreactor as an effective in vitro culture system for the micropropagation of Prunus spp. rootstocks and analysis of the plant-PGPMs interactions

Cantabella, Daniel; Mendoza, Carlos Rolando; Teixidó, Neus; Vilaró, F.; Dolcet-Sanjuan, Ramón

doi:10.1016/j.scienta.2021.110622

Cited by 8 publications

(9 citation statements)

References 64 publications

(79 reference statements)

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“…Even with a fully prescribed/defined protocol, in these conditions production is limited by the size of containers and physical manipulation restrictions [21] and is not easily scaled up and commercialized. To date, the use of liquid medium for the micropropagation of Prunus species has been limited to P. avium [93], P. armeniaca [94], P. cerasifera [95] and a hybrid rootstock of P. cerasifera and P. dulcis [96]. Published results are consistent with our higher proliferation rates found in temporary immersion bioreactors compared with semisolid medium.…”

Section: Discussionsupporting

confidence: 88%

Micropropagation of Plum (Prunus domestica L.) in Bioreactors Using Photomixotrophic and Photoautotrophic Conditions

et al. 2022

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In this study, we propagated two old Galician plum varieties in liquid medium using a temporary immersion system with RITA© bioreactors. Environmental variables including culture system, light intensity, CO2 enrichment, immersion frequency and sucrose supplementation were evaluated in relation to in vitro proliferation, physiological status and ex vitro performance. Bioreactors were superior to jars for culturing shoots in photomixotrophic conditions, producing up to 2 times more shoot numbers and up to 1.7 times more shoot length (depending on the genotype) using shoot clusters. The number and quality of shoots were positively influenced by the sucrose concentration in the medium, plus by the light and gaseous environment. For individual apical sections the best response occurred with 3% sucrose, 150 µmol m−2 s−1 photosynthetic photon flux density and 2000 ppm CO2, averaging 2.5 shoots per explant, 26 mm shoot length and 240 mm2 leaf area, while with 50 µmol m−2 s−1 light and ambient CO2 (400 ppm) values decreased to 1.2 shoots per explant, 14 mm of shoot length and 160 mm2 of leaf area. Shoots cultured photoautotrophically (without sucrose) were successfully rooted and acclimated despite of showing limited growth, low photosynthetic pigments, carbohydrate, phenolic and antioxidant contents during the multiplication phase.

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Section: Discussionsupporting

confidence: 88%

Micropropagation of Plum (Prunus domestica L.) in Bioreactors Using Photomixotrophic and Photoautotrophic Conditions

et al. 2022

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“…As reported in previous works [41][42][43][44][45][46], autotrophic in vitro culture is facilitated by aeration in the culture vessel and increasing the PAR radiation without stressing the plant materials. GreenTray ® 's design is particularly focused on improving aeration through two mechanisms, suction from the glass bottle containing the medium or the propulsion of air into the glass culture jar.…”

Section: Discussionmentioning

confidence: 96%

“…By pulling the holding racks, plantlet roots can be observed, sampled, or pictured the same way as the aerial part of the plants. Since the container holding the culture medium is independent from the cultured plantlets, it can be aliquoted, supplemented, inoculated, and observed at any time during culture [39,42]. These characteristics make the GreenTray ® TIS superior to other commercial TIS bioreactors and containers to perform studies on abiotic [43][44][45][46] or biotic [47][48][49][50][51][52][53][54][55] stress agents.…”

Section: Discussionmentioning

confidence: 99%

GreenTray®, a TIS Bioreactor for Plant Micropropagation and Abiotic or Biotic Stress Bioassays

Dolcet-Sanjuan,

Casanovas,

Franquesa

et al. 2024

Applied Sciences

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The temporary immersion system bioreactor named GreenTray® presents advantages over the existing ones. Firstly, there is no need to use forceps to move the shoots or plantlets in or out of the recipient, nor is the use of a scalpel necessary to divide them. Secondly, the basis of the shoot can remain in the GreenTray® and can sprout again over several cycles of growth. These two characteristics reduce the costs in the process of micropropagation. In addition, shoot or plantlet development is enhanced by the aeration of the recipient, thus enriching the recipient’s atmospheric CO2. This article describes and provides images and values of interest in (1) the micropropagation process of some commercial Prunus or Pyrus rootstocks; (2) the plantlet growth under autotrophic conditions; and (3) the inoculation of plants with pathogens that reproduce in vitro the symptoms observed in vivo, using the GreenTray® bioreactor.

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“…For instance, in Arabidopsis thaliana seedlings, studies have demonstrated that IAA-producing bacteria are able to induce root plasticity stimulating lateral root development (Contesto et al 2010;Iqbal and Hasnain 2013;Zamioudis et al 2013). In other studies using in vitro fruit tree plants inoculated with P. oryzihabitans, it has been suggested that changes in the content of auxins in the culture medium might be related with a higher number of roots (Cantabella et al 2022). As a result, root morphological changes induced by the PGPMs-produced IAA lead to an enhancement of nutrient uptake from the soil or root exudation (Spaepen and Vanderleyden 2011;Masciarelli et al 2013).…”

Section: Effects Of Pgpms In In Vitro Rootingmentioning

confidence: 99%

“…Nevertheless, the introduction of beneficial microorganisms into in vitro culture with the aim to remove the presence of endophytic contaminations has not been much considered. In experiments using the temporary immersion system GreenTray® bioreactor (Dolcet-Sanjuan and Mendoza, 2018 and 2020) the ability of P. oryzihabitans PGP01 and C. ramotenellum PGP02 to control the growth of endophytic contaminations in Prunus Rootpac® 20 shoots IBA-root induced was analysed (Cantabella et al 2022). In the latter study, P. oryzihabitans PGP01 was not able to control endophytes population in RP-20 explants at the pH commonly used for in vitro culture.…”

Section: In Vitro Biological Control Activity By Pgpmsmentioning

confidence: 99%

Using plant growth-promoting microorganisms (PGPMs) to improve plant development under in vitro culture conditions

Cantabella¹,

Dolcet-Sanjuan²,

Teixidó³

2022

Planta

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Plant in vitro culture techniques are highly useful to obtain significant amounts of true-to-type and disease-free plant materials. One of these techniques is clonal micropropagation which consists on the establishment of shoot tip cultures, shoot multiplication, in vitro rooting and acclimatization to ex vitro conditions. However, in some cases, the existence of recalcitrant genotypes, with a compromised multiplication and rooting ability, or the difficulties to overcome the overgrowth of endophytic contaminations might seriously limit its efficiency. In this sense, the establishment of beneficial interactions between plants and plant growth-promoting microorganisms (PGPMs) under in vitro culture conditions might represent a valuable approach to efficiently solve those restrictions. During the last years, significant evidence reporting the use of beneficial microorganisms to improve the yield of in vitro multiplication or rooting as well as their acclimatization to greenhouse or soil conditions have been provided. Most of these positive effects are strongly linked to the ability of these microorganisms to provide in vitro plants with nutrients such as nitrogen or phosphorous, to produce plant growth regulators, to control the growth of pathogens or to mitigate stress conditions. The culture of A. thaliana under aseptic conditions has provided high-quality knowledge on the root development signalling pathways, involving hormones, triggered in the presence of PGPMs. Overall, the present article offers a brief overview of the use of microorganisms to improve in vitro plant performance during the in vitro micropropagation stages, as well as the main mechanisms of plant growth promotion associated to these microorganisms. Main conclusion:The use of beneficial microorganisms improves the performance of in vitro cultured plants through the improvement of plant nutrition, the biological control of microbial pathogens or the production of phytohormones that promote plant growth and development.

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GreenTray® TIS bioreactor as an effective in vitro culture system for the micropropagation of Prunus spp. rootstocks and analysis of the plant-PGPMs interactions

Cited by 8 publications

References 64 publications

Micropropagation of Plum (Prunus domestica L.) in Bioreactors Using Photomixotrophic and Photoautotrophic Conditions

Micropropagation of Plum (Prunus domestica L.) in Bioreactors Using Photomixotrophic and Photoautotrophic Conditions

GreenTray®, a TIS Bioreactor for Plant Micropropagation and Abiotic or Biotic Stress Bioassays

Using plant growth-promoting microorganisms (PGPMs) to improve plant development under in vitro culture conditions

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