18The livestock industry is one of the main contributors to greenhouse gas emissions and there is 19 an increasing demand for the industry to reduce its carbon footprint. Several studies have shown 20 that feed additives 3-nitroxypropanol and nitrate to be effective in reducing enteric methane 21 emissions. The objective of this study was to estimate the net mitigating effect of using 3-22 nitroxypropanol and nitrate on total greenhouse gas emissions in California dairy industry. A life 23 cycle assessment approach was used to conduct a cradle-to-farm gate environmental impact 24 analysis based on dairy production system in California. Emissions associated with crop 25 production, feed additive production, enteric methane, farm management, and manure storage 26 were calculated and expressed as kg CO 2 equivalents (CO 2 e) per kg of energy corrected milk. 27 The total greenhouse gas emissions from baseline, two 3-nitroxypropanol and three nitrate 28 scenarios were 1.12, 0.993, 0.991, 1.08, 1.07, and 1.09 kg CO 2 e/kg energy corrected milk. The 29 average net reduction rates for 3-nitroxypropanol and nitrate were 11.7% and 3.95%, 30 respectively. In both cases, using the feed additives on the whole herd slightly improved overall 31 carbon footprint reduction compared to limiting its use during lactation phase. Although both 3-32 nitroxypropanol and nitrate had effects on decreasing the total greenhouse gas emission, the 33 former was much more effective with no known safety issues in reducing the carbon footprint of 34 dairy production in California. 35 Introduction 37The main greenhouse gases (GHG) emissions from agricultural food production include nitrous 38 oxide (N 2 O), carbon dioxide (CO 2 ) and methane (CH 4 ). Livestock sector contributes to 3 39 approximately 14.5% of global anthropogenic GHG emissions with 80% attributed to CH 4 40 production from enteric fermentation and manure management from ruminants [1]. Dairy 41 production is the third largest agricultural industry in the United States with total milk production 42 increasing 13% over the past decade reaching over 215 billion pounds in 2019 [2]. California, as 43 the top dairy production state accounted for over 20% of the total milk production with 1.73 44 million cows [2, 3]. 45 The dairy industry has an environmental impact including GHG emissions related to crop 46 production, enteric and manure CH 4 , water resource for feed production, excretion of nitrogen 47 and phosphorus, and land management [4]. There are several mitigation strategies developed to 48 reduce GHG emission from dairy, but most are modest in magnitude and some not applicable to 49 California [5]. In the last few years, several feed additives have been developed to reduce enteric 50 CH 4 emissions with varying results. Two of the most studied feed additives that substantially 51 reduce enteric CH 4 emissions include 3-nitroxypropanol (3NOP) and nitrate (e.g., [6][7][8][9]). 3-52 nitroxypropanol (also known as Bovaer in Europe), is a synthetic compound that inhibi...