Greenhouse and field experiments with winter oilseed rape cultivars resistant to Plasmodiophora brassicae Wor.

Řičařová, Veronika; Kazda, Jan; Baranyk, P.; Ryšánek, P.

doi:10.1016/j.cropro.2016.10.009

Cited by 4 publications

(7 citation statements)

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“…Two B. napus cultivars with different resistance against P. brassicae pathotype 6 were analyzed in the respect to disease severity as well as to their responses to treatment with defense hormones. The cultivar SY Alister has been previously reported as resistant to P. brassicae infection [20]. The plants were treated by foliar spray with SA at the day of inoculation, or in the second set of experiments at the day of inoculation, and subsequently, 15 dai, which corresponded to the onset of gall formation.…”

Section: Resultsmentioning

confidence: 99%

Hormonal Responses to Plasmodiophora brassicae Infection in Brassica napus Cultivars Differing in Their Pathogen Resistance

Přerostová

Dobrev

Konradyová

et al. 2018

IJMS

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Hormonal dynamics after Plasmodiophora brassicae infection were compared in two Brassica napus cultivars—more resistant SY Alister and more sensitive Hornet, in order to elucidate responses associated with efficient defense. Both cultivars responded to infection by the early transient elevation of active cytokinins (predominantly cis-zeatin) and auxin indole-3-acetic acid (IAA) in leaves and roots, which was longer in Hornet. Moderate IAA levels in Hornet roots coincided with a high expression of biosynthetic gene nitrilase NIT1 (contrary to TAA1, YUC8, YUC9). Alister had a higher basal level of salicylic acid (SA), and it stimulated its production (via the expression of isochorismate synthase (ICS1)) in roots earlier than Hornet. Gall formation stimulated cytokinin, auxin, and SA levels—with a maximum 22 days after inoculation (dai). SA marker gene PR1 expression was the most profound at the time point where gall formation began, in leaves, roots, and especially in galls. Jasmonic acid (JA) was higher in Hornet than in Alister during the whole experiment. To investigate SA and JA function, SA was applied before infection, and twice (before infection and 15 dai), and JA at 15 dai. Double SA application diminished gall formation in Alister, and JA promoted gall formation in both cultivars. Activation of SA/JA pathways reflects the main differences in clubroot resistance.

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Section: Resultsmentioning

confidence: 99%

Hormonal Responses to Plasmodiophora brassicae Infection in Brassica napus Cultivars Differing in Their Pathogen Resistance

Přerostová

Dobrev

Konradyová

et al. 2018

IJMS

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“…A few studies have investigated the spatial patterns of P. brassicae at a field level, but (to our knowledge) none has evaluated changes in these patterns over time, nor their relationship with soil chemical properties. A patchy spatial pattern has been described for the pathogen in fields in Canada, Sweden and the Czech Republic, based on anecdotal observations, descriptive statistics [45,48] and Spatial Analysis by Distance Indices (SADIE) [46,47]. Clubroot also occurs more frequently at field entrances [45,46] and margins [47].…”

Section: Discussionmentioning

confidence: 99%

“…A patchy spatial pattern has been described for the pathogen in fields in Canada, Sweden and the Czech Republic, based on anecdotal observations, descriptive statistics [45,48] and Spatial Analysis by Distance Indices (SADIE) [46,47]. Clubroot also occurs more frequently at field entrances [45,46] and margins [47]. Similarly, in the current analysis, a patchy pattern was found with respect to P. brassicae inoculum density in the evaluated fields, with most patches located at the field edges adjacent to the entrance.…”

Section: Discussionmentioning

confidence: 99%

“…Soilborne diseases are characterized by aggregated spatial patterns, with dynamic patches that vary in size over time, and which may reflect local soil conditions that favor the disease or inhibit crop growth [39,43,44]. Observations of in-field clubroot spatial patterns have indicated that disease incidence is higher at the field entrances [45,46] or field margins [47,48], suggesting that disease spread occurs mainly through the movement of infested soil on farm machinery [45]. However, while it is widely known that clubroot tends to have a patchy spatial pattern [45,46,48], detailed evaluations of within field spread over time or the effect of soil properties on inoculum density have not been conducted.…”

Section: Introductionmentioning

confidence: 99%

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Plasmodiophora brassicae Inoculum Density and Spatial Patterns at the Field Level and Relation to Soil Characteristics

2021

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Clubroot, caused by Plasmodiophora brassicae, is an important soilborne disease of the Brassicaceae. Knowledge of the spatial dynamics of P. brassicae at the field level and the influence of soil properties on pathogen spatial patterns can improve understanding of clubroot epidemiology and management. To study the spatial patterns of P. brassicae inoculum density and their relationship to different soil properties, four clubroot-infested fields in central Alberta, Canada, were sampled in 2017 and 2019, and P. brassicae inoculum density, soil pH, and boron, calcium, and magnesium concentrations were quantified. Spatial autocorrelation of the inoculum density was estimated for each of the fields in both years with the Moran’s I and semi-variograms. A Bayesian hierarchical spatial approach was used to model the relationship between P. brassicae inoculum density and the soil parameters. Patchiness of the pathogen was detected, with most patches located at the field edges and adjacent to the entrance. Infested patches grew in size from 2017 to 2019, with an average increase in diameter of 221.3 m and with this growth determined by the maximum inoculum density and active dispersal methods such as movement by machinery and wind. Soil pH, boron, calcium, and magnesium concentrations were not found to have an important effect on the inoculum density of P. brassicae.

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“…The clubroot disease is difficult to control, once the soil is infested with spores of P. brassicae , the soil contamination could last for more than 20 years (Dixon 2009). In the last decade, this disease is spreading rapidly (Ricarova et al 2017). In the light of seriousness, numerous studies have been conducted to discover resistance genes.…”

Section: Discussionmentioning

confidence: 99%

Analysing the genetic architecture of clubroot resistance variation in Brassica napus by associative transcriptomics

Hejna

Havlíčková

et al. 2019

Mol Breeding

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Clubroot is a destructive soil-borne pathogen of Brassicaceae that causes significant recurrent reductions in yield of cruciferous crops. Although there is some resistance in oilseed rape (a crop type of the species Brassica napus ), the genetic basis of that resistance is poorly understood. In this study, we used an associative transcriptomics approach to elucidate the genetic basis of resistance to clubroot pathotype ECD 17/31/31 across a genetic diversity panel of 245 accessions of B. napus . A single nucleotide polymorphism (SNP) association analysis was performed with 256,397 SNPs distributed across the genome of B. napus and combined with transcript abundance data of 53,889 coding DNA sequence (CDS) gene models. The SNP association analysis identified two major loci (on chromosomes A2 and A3) controlling resistance and seven minor loci. Within these were a total of 86 SNP markers. Altogether, 392 genes were found in these regions. Another 21 genes were implicated as potentially involved in resistance using gene expression marker (GEM) analysis. After GO enrichment analysis and InterPro functional analysis of the identified genes, 82 candidate genes were identified as having roles in clubroot resistance. These results provide useful information for marker-assisted breeding which could lead to acceleration of pyramiding of multiple clubroot resistance genes in new varieties. Electronic supplementary material The online version of this article (10.1007/s11032-019-1021-4) contains supplementary material, which is available to authorized users.

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Greenhouse and field experiments with winter oilseed rape cultivars resistant to Plasmodiophora brassicae Wor.

Cited by 4 publications

References 23 publications

Hormonal Responses to Plasmodiophora brassicae Infection in Brassica napus Cultivars Differing in Their Pathogen Resistance

Hormonal Responses to Plasmodiophora brassicae Infection in Brassica napus Cultivars Differing in Their Pathogen Resistance

Plasmodiophora brassicae Inoculum Density and Spatial Patterns at the Field Level and Relation to Soil Characteristics

Analysing the genetic architecture of clubroot resistance variation in Brassica napus by associative transcriptomics

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